Margaretha Herawati Master Program in Biomedical Sciences, Faculty of Medicine University of Indonesia
Department of Biochemistry, Faculty of Dentistry, Universitas Prof. Dr. Moestopo (Beragama), Jakarta Indonesia
. Wardaya Indonesian Air Force Institute of Aviation Medicine (Lembaga Kesehatan Penerbangan dan Ruang Angkasa) Saryanto, Jakarta Indonesia
Wawan Mulyawan Indonesian Air Force Institute of Aviation Medicine (Lembaga Kesehatan Penerbangan dan Ruang Angkasa) Saryanto, Jakarta Indonesia
Fanny Septiani Farhan Department of Physiology, Faculty of Medicine Muhammadiyah University, Jakarta Indonesia
Frans Ferdinal Department of Biochemistry and Molecular Biology, Faculty of Medicine Tarumanagara University, Jakarta Indonesia
Sri Widia A. Jusman Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine University of Indonesia, Jakarta
Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta Indonesia
Mohamad Sadikin Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine University of Indonesia, Jakarta
Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, Jakarta Indonesia
Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure
Margaretha Herawati, . Wardaya, Wawan Mulyawan, Fanny Septiani Farhan, Frans Ferdinal, Sri Widia A. Jusman, Mohamad Sadikin
Abstract
The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.