DNA BARCODING UNTUK VALIDASI SPESIES PAUS SPERMA (Physeter macrocephalus LINNAEUS, 1758) DARI PERAIRAN LAUT SAWU, NUSA TENGGARA TIMUR, INDONESIA

  • Agus Alim Hakim Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
  • Endah Sri Rahayu Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
  • Ali Mashar Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
  • Nurlisa Alias Butet Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
  • Yusli Wardiatno Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
  • Mohammad Mukhlis Kamal Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, IPB University, 16680, Indonesia
DOI: https://doi.org/10.29244/coj.v7i1.49702
Keywords: COI, DNA barcoding, genetic, Sawu Sea, sperm whale

Abstract

The sperm whale (Physeter macrocephalus) is a protected marine biota in Indonesia. Molecular identification of sperm whales from the Sawu Sea (East Nusa Tenggara, Indonesia) was carried out using the 16S rRNA gene. This study aims to validate the species of the sperm whale (Physeter macrocephalus) in Indonesia using the partial cytochrome oxidase subunit I (COI) gene. The specimens used were tissue specimens from laboratory collections taken from Sawu Sea waters in 2014. The three samples were validated as Physeter microcephalus with 100% similarity. The base composition is dominated by the base thymine (31.7–31.8%) with a GC content of 42.7–42.8%. The genetic distance for the same species was 0.0015, while the highest genetic distance for Orcaella brevirostris was 0.2128. Molecular identification using the 16S rRNA gene and COI has validated the species of a sperm whale (Physeter macrocephalus) in Indonesia so that this genetic data can be used as a basis for sperm whale resource management and conservation.

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Published
2023-10-24
Issue
Vol. 7 No. 1 (2023): COJ (Coastal and Ocean Journal)
Section
Articles