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Submitted
Feb 3, 2024
Accepted
Feb 3, 2024
Published
Feb 8, 2022
Pengembangan Uji Berbasis Molekuler Protamine-1 (Prm1) Sebagai Penentu Tingkat Fertilitas untuk Proses Seleksi dan Kebijakan Pengafkiran Sapi Pejantan Unggul di Indonesia
Corresponding Author(s) : B Purwantara
purwantara@apps.ipb.ac.id
Policy Brief Pertanian, Kelautan, dan Biosains Tropika,
Vol. 4 No. 4 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Abstract
Standar kualitas semen yang berperan penting dalam keberhasilan inseminasi buatan (IB) sebagai upaya peningkatan populasi dan mutu ternak sapi di Indonesia terbukti tidak seutuhnya mampu menentukan tingkat fertilitas sapi pejantan di Indonesia. Studi terbaru membuktikan bahwa meskipun sudah memenuhi standar kualitas semen yang sudah ditetapkan, namun tiga dari enam ekor sapi pejantan di Indonesia masih tergolong fertilitas rendah (<60%). Potamine-1 (PRM1) merupakan molekul pada inti spermatozoa yang telah terbukti memiliki hubungan erat dengan fungsi fertilitas dan kualitas spermatozoa pada berbagai sapi pejantan di Indonesia. Hal tersebut mendasari usulan pengembangan uji berbasis molekuler PRM1 sebagai penentu tingkat fertilitas yang diharapkan dapat digunakan untuk proses seleksi dan kebijakan pengafkiran sapi pejantan unggul di Indonesia. Melalui policy brief ini diharapkan usulan pengembangan uji tersebut dapat lebih tersampaikan dengan segala solusi yang diberikan, termasuk terkait pendekatan uji berbasis molekuler yang paling tepat digunakan, kajian-kajian lanjutan, dan berbagai harapan dukungan dari segala pihak demi terwujudnya pengembangan uji PRM1.
Keywords
Uji Molekuler
Inseminasi Buatan
Fertilitas
Sapi Pejantan
PurwantaraB., PardedeB., AgilM., SupriatnaI., KarjaN., ArifiantiniR., & SumantriC. (2022). Pengembangan Uji Berbasis Molekuler Protamine-1 (Prm1) Sebagai Penentu Tingkat Fertilitas untuk Proses Seleksi dan Kebijakan Pengafkiran Sapi Pejantan Unggul di Indonesia. Policy Brief Pertanian, Kelautan, Dan Biosains Tropika, 4(4), 367-374. https://doi.org/10.29244/agro-maritim.0404.367-374
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References
References
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Aoki VW, Carrell DT. 2003. Human protamines and the developing spermatid: their structure, function, expression and relationship with male infertility. Asian J Androl. 5:315–324.
Beletti ME, Costa LF, Guardieiro MM. 2005.Morphometric features and chromatin condensation abnormalities evaluated by toluidine blue staining in bull spermatozoa. Braz J Morphol Sci. 22.
Boerke A, Dieleman SJ, Gadella BM. 2007. Apossible role for sperm RNA in early embryo development. Theriogenology. 68(Suppl.1): S147–S155. https://doi.org/10.1016/j.theriogenology.2007.05.058
Bower PA, Yelick PC, Hecht NB. 1987. Both P1 and P2 protamine genes are expressed inmouse, hamster, and rat. Biol Reprod. 37:479–488. https://doi.org/10.1095/biolreprod37.2.479
Corzett M, Mazrimas J, Balhorn R. 2002. Protamine 1: protamine 2 stoichiometry in the sperm of eutherian mammals. Mol Reprod Dev. 61:519–527. https://doi.org/10.1002/mrd.10105
Depa-Martynow M, Kempisty B, Jagodzinski PP, Pawelzyk L, Jedrzejczak P. 2011. Impact ofprotamine transcripts and their proteins on the quality and fertilization ability of spermand the development of preimplantation embryos. Reprod Biol. 12:57-72. https://doi.org/10.1016/S1642-431X(12)60077-1
Dogan S, Vargovic P, Oliveira R, Belser LE, Kaya A, Moura A, Sutovsky P, Parrish J, Topper E,Memili E. 2015. Sperm protamine-status correlates to the fertility of breeding bulls. Biol Reprod.1(4):1-9. https://doi.org/10.1095/biolreprod.114.124255
Feugang JM, Rodriguez-Osorio N, Kaya A, WangH, Page G, Ostermeier GC, Topper EK, Memili E. 2010. Transcriptome analysis of bull spermatozoa: implications for male fertility. Reprod Biomed Online. 21:312–324. https://doi.org/10.1016/j.rbmo.2010.06.022
Ganguly I, Gaur GK, Kumar S, Mandal DK, Kumar M, Singh U, Kumar S, Sharma A. 2013. Differential expression of protamine 1 and 2 genes in mature spermatozoa of normal and motility impaired semen producing crossbred Frieswal (HF x Sahiwal) bulls. Res Vet Sci. 94:245-62. https://doi.org/10.1016/j.rvsc.2012.09.001
Hamilton TRS, Simoes R, Mendes CM, Goissis MD, Nakajima E, Martins EAL, Vicintin JA, Assumpcao MEOA. 2019. Detection of protamine 2 in bovine spermatozoa and testicles. Andrology. 7:373-381. https://doi.org/10.1111/andr.12610
Maier WM, Nussbaum G, Domenjoud L, Klemm U, Engel W. 1990. The lack of protamine 2 (P2) in boar and bull spermatozoa is due tomutations within the P2 gene. Nucleic Acids Res. 18:1249–1254. https://doi.org/10.1093/nar/18.5.1249
Olivia R. 2006. Protamines and male infertility. Hum Rerod Update.12(4): 417-35. https://doi.org/10.1093/humupd/dml009
Pardede BP, Agil M, Karja NWK, Sumantri C, Supriatna I, Purwantara B. 2022. PRM1 gene expression and its protein abundance infrozen-thawed spermatozoa as potential fertility markers in breeding bulls. Vet Sci. 9(3): 111. https://doi.org/10.3390/vetsci9030111
Pardede BP, Maulana T, Kaiin EM, Agil M, Karja NWK, Sumantri C, Supriatna I. 2021. The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia. Vet World. 14(9): 2473-2481. https://doi.org/10.14202/vetworld.2021.2473-2481
Pardede BP, Supriatna I, Agil M. 2020. Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility. Vet World.13:556-562. https://doi.org/10.14202/vetworld.2020.556-562
Pardede BP, Supriatna I, Yudi Y, Agil M. 2020. Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program. Vet World. 13:2649-2657. https://doi.org/10.14202/vetworld.2020.2649-2657
Prakash MA, Kumaresan A, Sinha MK, Kamaraj E, Mohanty TK, Datta TK, Morrell JM. 2020. RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs incrossbred bull spermatozoa. Anim Reprod Sci. 222:106621. https://doi.org/10.1016/j.anireprosci.2020.106621
Rosyada ZNA, Ulum MF, Tumbelaka LITA, Purwantara B. 2020. Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia. Vet World. 13(5): 947-955. https://doi.org/10.14202/vetworld.2020.947-955
Schneider S, Balbach M, Jikeli JF, Fietz D, Nettersheim D, Jostes S, Schmidt R, Kressin M, Bergmann M, Wachten D, StegerK,Schorle H. 2016. Re-visiting the protamine-2 locus: deletion, but not haploinsufciency, renders male mice infertile. Sci Rep. 6:36764. https://doi.org/10.1038/srep36764
Seli E, Gardner DK, School craft WB, Moffatt O, Sakkas D. 2004. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fer Ster. (82): 378-383. https://doi.org/10.1016/j.fertnstert.2003.12.039
Takeda N, Yoshinaga K, Furushima K, Takamune K, Li Z, Abe S, Aizawa S, Yamamura K. 2016. Viable offspring obtained from Prm1-defcient sperm in mice. Sci Rep. 6:27409. https://doi.org/10.1038/srep27409
Virro MR, Larson-Cook KL, Evenson DP. 2004. Sperm chromatin structure assay (SCSA) parameters are related to fertilization blastocyst development, and ongoing pregnancy in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril. (81): 1289-1295. https://doi.org/10.1016/j.fertnstert.2003.09.063
Aoki VW, Carrell DT. 2003. Human protamines and the developing spermatid: their structure, function, expression and relationship with male infertility. Asian J Androl. 5:315–324.
Beletti ME, Costa LF, Guardieiro MM. 2005.Morphometric features and chromatin condensation abnormalities evaluated by toluidine blue staining in bull spermatozoa. Braz J Morphol Sci. 22.
Boerke A, Dieleman SJ, Gadella BM. 2007. Apossible role for sperm RNA in early embryo development. Theriogenology. 68(Suppl.1): S147–S155. https://doi.org/10.1016/j.theriogenology.2007.05.058
Bower PA, Yelick PC, Hecht NB. 1987. Both P1 and P2 protamine genes are expressed inmouse, hamster, and rat. Biol Reprod. 37:479–488. https://doi.org/10.1095/biolreprod37.2.479
Corzett M, Mazrimas J, Balhorn R. 2002. Protamine 1: protamine 2 stoichiometry in the sperm of eutherian mammals. Mol Reprod Dev. 61:519–527. https://doi.org/10.1002/mrd.10105
Depa-Martynow M, Kempisty B, Jagodzinski PP, Pawelzyk L, Jedrzejczak P. 2011. Impact ofprotamine transcripts and their proteins on the quality and fertilization ability of spermand the development of preimplantation embryos. Reprod Biol. 12:57-72. https://doi.org/10.1016/S1642-431X(12)60077-1
Dogan S, Vargovic P, Oliveira R, Belser LE, Kaya A, Moura A, Sutovsky P, Parrish J, Topper E,Memili E. 2015. Sperm protamine-status correlates to the fertility of breeding bulls. Biol Reprod.1(4):1-9. https://doi.org/10.1095/biolreprod.114.124255
Feugang JM, Rodriguez-Osorio N, Kaya A, WangH, Page G, Ostermeier GC, Topper EK, Memili E. 2010. Transcriptome analysis of bull spermatozoa: implications for male fertility. Reprod Biomed Online. 21:312–324. https://doi.org/10.1016/j.rbmo.2010.06.022
Ganguly I, Gaur GK, Kumar S, Mandal DK, Kumar M, Singh U, Kumar S, Sharma A. 2013. Differential expression of protamine 1 and 2 genes in mature spermatozoa of normal and motility impaired semen producing crossbred Frieswal (HF x Sahiwal) bulls. Res Vet Sci. 94:245-62. https://doi.org/10.1016/j.rvsc.2012.09.001
Hamilton TRS, Simoes R, Mendes CM, Goissis MD, Nakajima E, Martins EAL, Vicintin JA, Assumpcao MEOA. 2019. Detection of protamine 2 in bovine spermatozoa and testicles. Andrology. 7:373-381. https://doi.org/10.1111/andr.12610
Maier WM, Nussbaum G, Domenjoud L, Klemm U, Engel W. 1990. The lack of protamine 2 (P2) in boar and bull spermatozoa is due tomutations within the P2 gene. Nucleic Acids Res. 18:1249–1254. https://doi.org/10.1093/nar/18.5.1249
Olivia R. 2006. Protamines and male infertility. Hum Rerod Update.12(4): 417-35. https://doi.org/10.1093/humupd/dml009
Pardede BP, Agil M, Karja NWK, Sumantri C, Supriatna I, Purwantara B. 2022. PRM1 gene expression and its protein abundance infrozen-thawed spermatozoa as potential fertility markers in breeding bulls. Vet Sci. 9(3): 111. https://doi.org/10.3390/vetsci9030111
Pardede BP, Maulana T, Kaiin EM, Agil M, Karja NWK, Sumantri C, Supriatna I. 2021. The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia. Vet World. 14(9): 2473-2481. https://doi.org/10.14202/vetworld.2021.2473-2481
Pardede BP, Supriatna I, Agil M. 2020. Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility. Vet World.13:556-562. https://doi.org/10.14202/vetworld.2020.556-562
Pardede BP, Supriatna I, Yudi Y, Agil M. 2020. Relationship of frozen-thawed semen quality with the fertility rate after being distributed in the Brahman Cross Breeding Program. Vet World. 13:2649-2657. https://doi.org/10.14202/vetworld.2020.2649-2657
Prakash MA, Kumaresan A, Sinha MK, Kamaraj E, Mohanty TK, Datta TK, Morrell JM. 2020. RNA-Seq analysis reveals functionally relevant coding and non-coding RNAs incrossbred bull spermatozoa. Anim Reprod Sci. 222:106621. https://doi.org/10.1016/j.anireprosci.2020.106621
Rosyada ZNA, Ulum MF, Tumbelaka LITA, Purwantara B. 2020. Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia. Vet World. 13(5): 947-955. https://doi.org/10.14202/vetworld.2020.947-955
Schneider S, Balbach M, Jikeli JF, Fietz D, Nettersheim D, Jostes S, Schmidt R, Kressin M, Bergmann M, Wachten D, StegerK,Schorle H. 2016. Re-visiting the protamine-2 locus: deletion, but not haploinsufciency, renders male mice infertile. Sci Rep. 6:36764. https://doi.org/10.1038/srep36764
Seli E, Gardner DK, School craft WB, Moffatt O, Sakkas D. 2004. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fer Ster. (82): 378-383. https://doi.org/10.1016/j.fertnstert.2003.12.039
Takeda N, Yoshinaga K, Furushima K, Takamune K, Li Z, Abe S, Aizawa S, Yamamura K. 2016. Viable offspring obtained from Prm1-defcient sperm in mice. Sci Rep. 6:27409. https://doi.org/10.1038/srep27409
Virro MR, Larson-Cook KL, Evenson DP. 2004. Sperm chromatin structure assay (SCSA) parameters are related to fertilization blastocyst development, and ongoing pregnancy in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril. (81): 1289-1295. https://doi.org/10.1016/j.fertnstert.2003.09.063