KERAGAMAN GEN CALPASTATIN, CALPAIN 3 DAN MYOSTATIN PADA DOMBA DI UP3 JONGGOL
Abstract
The aim of this study was to identify the genetic polymorphisms of calpastatin (CAST), calpain 3 (CAPN3) and myostatin (MSTN) on local sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU). A total number of 294 blood samples were collected from JASTRU. The identification of polymorhism in CAST and CAPN3 genes performed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) while MSTN gene by using PCR-SSCP methods. The results showed that CAST|MspI, CAST|NcoI and CAPN3|MaeII loci were polymorphic, whereas The MSTN locus was monomorphic for G (1.0). The frequency of allele M (0.87) on the locus (CAST|MspI) higher than the N allele (0.13). At locus CAST|NcoI, the frequency of allele M (0.96) higher than the N allele (0.04). At the CAPN3|MaeII, allele G (0.85) and allele T (0.15). Locus CAST|NcoI has higher observed heterozygosity (Ho = 0.92) compared to CAPN3|MaeII and CAST|MspI (Ho = 0.74-0.77), however has lower compared to CAPN3|MaeII and CAST|MspI in expected of heterozygosity (He = 0.08 vs 0.23-0.26) and in index fixation (Fis = -0.04 vs 0.03-0.12).
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References
Andreas, E., C. Sumantri, H. Nuraini, A. Farajallah, dan A. Anggraeni. 2010. Identification of GH|AluI and GHR|AluI Genes Polymorphisms in Indonesian Buffalo. JITAA. 35:215-221.
Bishop, M.D., M. Koohmaraie, J.Killefer, dan S. Kappes.1993. Restriction fragment length polymorphisms of the bovine calpastatin gene. J. Anim. Sci. 71: 2277.
Boman, I.A., G. Klemetsda, T. Blichfeldt, O. Nafstad, dan D. I. Vage. 2009. A frameshift mutation in the coding region of the myostatin gene (MSTN) affects carcass conformation and fatness in Norwegian White Sheep (Ovis aries). Anim.Genet. 40:418-422.
Byun, S.O., Q. Fang, H. Zhou, dan J.G.H. Hickford. 2009. An effective method for silver staining DNA in large numbers of polyacrylamide gels. Anal.Biochem. 385:174-175.
Gillespie, J. H. 1998. Population Genetics, A Concies Guide. The Johns Hopkins University Press. London.
Goll, D.E., V.F. Thompson, H. Li, W. Wei, dan J. Cong. 2003. The calpain system. Physiol. Rev. 83:731-801.
Grobet, L., L.J. Martin, D. Poncelet, D. Pirottin, B. Brouwers, J. Riquet, A. Schoeberlein, S. Dunner, F. Ménissier, J. Massabanda, R. Fries, R. Hanset, dan M. Georges. 1997. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nature Genet. 17:71-74.
Hartl, D.L. 1987. A Primer of Population Genetics. 2nd Ed. Sinauer Associates, Inc.USA.
Hediger, R., H. A. Ansari, dan G. F. Stranzinger. 1991. Chromosome banding and gene localizations support extensive conservation of chromosome structure between cattle and sheep. Cytogenet. Cell Genet. 57:127Huang, Y & K.K.W. Wang. 2001. The calpain family and human disease. Trends. Mol. Med. 7:355-362.
Kappes, S.M., J.W. Keele, R.T. Stone, T.S.Sonstegard, T.P.L. Smith, R.A. Mcgraw, N.L.Lopezcorrales, dan C.W.Beattie. 1997. A second-generation linkage map of the bovine genome. Genome Res. 7:235.
Kidd, V.J., J.M. Lahti, dan T. Teitz. 2000. Proteolytic regulation of apoptosis. Seminar in. Cell. Dev. Biol. 11:191-201.
Marson, E.P., J.B.S. Ferraz, F.V. Meirelles, J.C.C. Balieiro, J.P. Eler, L.G.G. Figuerido, dan G.B. Mourao. 2005. Genetic characterization of European-Zebu composite bovine using RFLP markers. Genet. Mol. Res. 4:496-505.
McPherron, A.C., A.M. Lawler, dan S.J. Lee. 1997. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature. 387:83-90.
Nei, M. and S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.
Palmer,B.R., N. Roberts, J.G.H. Hickford, dan R. Bickerstaffe. 1998. Rapid Communication: PCR- RFLP for MspI and NcoI in the ovine calpastatin gene. Am Soc Anim Sci. 76:1499-1500.
Raynaud, F., G. Carnac, A. Marcilhac, dan Y. Benyamin. 2004. m-Calpain implication in cell cycle during muscle precursor cell activation. Exp. Cell. Res. 298:48- 57.
Sambrook, J., dan D. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, United State of America.
Schuelke, M., K.R. Wagner, L.E. Stolz, C. Hubner, T. Riebel, W. Komen, T. Braun, J.F. Tobin, dan S.J. Lee. 2004. Brief report – Myostatin mutation associated with gross muscle hypertrophy in a child. N. Engel J. Med. 350: 2682-2688.
Sutikno, M. Yamin, dan C. Sumantri. 2011. Association of polymorphism Calpastatin gene with body weight of local sheep in Jonggol, Indonesia. Med. Pet. 34:1-6.
Sumantri. C., A. Einstiana, J. F. Salamena, dan I.Inounu. 2007. Keragaan dan hubungan phylogenik antar domba lokal di Indonesia melalui pendekatan analisis morfologi. JITV. 12: 42-54.
Sumantri, C., A. Farajallah, U. Fauzi, dan J.F. Salamena. 2008a. keragaman genetik DNA mikrosatelit dan hubungannya dengan performa bobot badan pada dpmba lokal. Med Pet. 31:1-13.
Sumantri, C., R. Diyono 1, A. Farajallah, dan I. Inounu. 2008b. Polimorfisme gen calpastatin (CAST-Msp1) dan pengaruhnya terhadap bobot badan pada domba local. JITV. 13:117-126.
Sumantri, C., D. Herdiana, A. Farajallah, D. Rahmat, dan A. Anggraeni. 2008c. Keragaman Gen (Pit-1-Hinf 1) dan Pengaruhnya terhadap Bobot Badan Induk dan Produksi Susu pada Domba Lokal di Unit Pendidikan, Penelitian Peternakan Jonggol (UP3J). JITV. 14:222-229.
Sumantri, C., E. Andreas, A. Farajallah, dan Jarmuji. 2008d. Keragaman gen к-kasein dan hubungannya dengan produksi dan kualitas susu pada domba di Unit Pendidikam dan Penelitian Peternakan (UP3) Jonggol. JIPI 13:49-55.
Sumantri C., Jakaria, M. Yamin, H. Nuraini, and Eryk Andreas. 2011. Identification of myostatin gene c.960delG locus polymorphism in Indonesian local sheep by using PCR-SSCP method. JITAA. 36:145-151.
Tambasco, D.D., C.C.P. Paz, M. Tambasco, Studart, A.P. Pereira, M.M. Alencar, A.R. Freitas, L.L. Coutinho, I.U. Packer, dan L.C.A. Regitano. 2003. Candidates genes for growth traits in beef cattle crosses Bos Taurus x Bos Indicus. J. Anim. Breed. Genet. 120:51-60.
Zhou, H., J.G.H. Hickford, dan Q. Fang. 2007. Single nucleotide polymorphisms of the ovine calpain 3 (CAPN3) gene. Mol. Cell Prob. 21:78-79.
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