Polimorfisme g.-371T>A Promotor Gen Miostatin pada Sapi Pedaging Indonesia

  • Sutikno Sutikno Jurusan Peternakan, Sekolah Tinggi Pertanian Kutai Timur, Jl. Soekarno Hatta, Tlk. Lingga, Sangatta Utara, Kutai Timur, Kalimantan Timur 75611
  • Rudy Priyanto Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
  • Cece Sumantri Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
  • Jakaria Jakaria Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680


Myostatin (MSTN) gene acts as a negative regulator of muscle growth. The aim of the present study was to identify polymorphism of g.-371T>A in promoter region of MSTN gene in Indonesian beef cattle. Blood samples were collected from 191 cattle, including Bali (BL) (42), Madura (MD) (20), Pesisir (PI) (17), Katingan (KT) (16), Ongole grade (PO) (22), Pasundan (PD) (14), Sumba ongole (SO) (10), Brahman (BH) (17), Simmental (SM) (15), and Limousin (LM) (18). Polymorphism of MSTN gene was analyzed using PCR-RFLP (DraI) and direct sequencing methods. Results of genotyping g-371T>A were polymorphic (TT, TA, and AA genotypes) in Simmental, SO, and Katingan. The frequencies of alleles T and A were 0.83; 0.90; 0.97 and 0.17; 0.10; 0.03 respectively. The values of Ho and He were 0.06–0.20 and 0.06–0.28, respectively. The results of this study are in Hardy-Weinberg equilibrium (P> 0.05). Whereas in PD, MD, PI, PO, BH, LM, and BL were monomorphic (TT genotype). The result of sequencing the promoter region of MSTN gene found that mutations transversion was occurred in T to A at g.-371. It was concluded that g.-371T>A of MSTN gene was polymorphic which was potential to be used as genetic markers of muscle growth in SM, SO, and KT cattle.

Keywords: SNP g.-371T>A of MSTN gene, Sumba ongole cattle, Katingan cattle, Simmental cattle


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Allendoft FW, Luikar G, Aitken SN. 2013. Conservation and the genetics of population. 2nd ed. Oxford (UK): Wiley-Blackwell.

Crisa A, Marchitelli C, Savarese MC, Valentini A. 2003. Sequence analysis of myostatin promoter in cattle. Cytogenet Genome Res. 102: 48–52. https://doi.org/10.1159/000075724

Gershon TJ, Kadonaga JT. 2010. Regulation of gene expression via the core promoter and the basal transcriptional machinery. Developmental Biology. 339: 225–229. https://doi.org/10.1016/j.ydbio.2009.08.009

Hall TA. 2011. BioEdit: An important software for molecular biology. GERF Bull Biosci. 2(1): 60-61.

Han SH, Cho IC, Ko MS, Kim EY, Park SP, Lee SS, Oh HS. 2012. A promoter polymorphism of mstn g.2371T>A and its associations with carcass traits in Korean cattle. Molecular Biology Reports. 39: 3767-3772. https://doi.org/10.1007/s11033-011-1153-z

Huang Z, Chen X, Chen D. 2011. Myostatin: A novel insight into its role in metabolism, signal pathways, and expression regulation. Cellular signalling. 23: 1441-1446. https://doi.org/10.1016/j.cellsig.2011.05.003

Keller I, Bensasson D, Nichols RA. 2007. Transition-transversion bias is not universal: a counter example from grasshopper pseudogenes. PLoS Genet 3(2): 0185-0191. https://doi.org/10.1371/journal.pgen.0030022

Lenhard B, Sandelin A, Carninci P. 2012. Metazoan promoters: emerging characteristics and insights into transcriptional regulation. Nature Reviews Genetics.. 13: 233–245. https://doi.org/10.1038/nrg3163

Marson EP, Ferraz JBS, Meirelles FV, Balieiro JCC, Eler JP, Figuerido LGG, Mourao GB. 2005. Genetic characterization of European-Zebu composite bovine using RFLP markers. Genetica Molecular Research. 4(3): 496-505.

Matukumalli LK, Lawley CT, Schnabel RD, Taylor JF, Allan MF, Heaton MP, O'Connell J, Moore SS, Smith TP, Sonstegard TS, Van Tassell CP. 2009. Development and characterization of a high density SNP genotyping assay for cattle. PLoS. 4(4):1–3. https://doi.org/10.1371/journal.pone.0005350

Miyake M, Hayashi S, Taketa Y, Iwasaki S, Watanabe K, Ohwada S, Aso H, Yamaguchi T. 2010. Myostatin down-regulates the igf-2 expression via alk-smad signaling during myogenesis in cattle. Journal of Animal Science. 81: 223–229. https://doi.org/10.1111/j.1740-0929.2009.00725.x

Nei M, Kumar S. 2000. Molecular Evolution and Phylogenetics. New York (US): Oxford University Press.

Ohler U, Wassarman DA. 2010. Promoting developmental transcription. Development. 137: 15-26. https://doi.org/10.1242/dev.035493

Sambrook J, Russell D. 2001. Molecular Cloning: a Laboratory Manual. 3rd ed. New York (US): Cold Spring Harbor Laboratory Press.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. 28(10): 2731-2739. https://doi.org/10.1093/molbev/msr121

Zhang RF, Chen H, Lei CZ, Zhang CL, Lan XY, Zhang YD, Zhang HJ, Bao B. Niu H, Wang XZ. 2007. association between polymorphisms of mstn and myf5 genes and growth traits in three chinese cattle breeds. AJAS. 20(12): 1798-1804. https://doi.org/10.5713/ajas.2007.1798

How to Cite
SutiknoS., PriyantoR., SumantriC., & JakariaJ. (2020). Polimorfisme g.-371T>A Promotor Gen Miostatin pada Sapi Pedaging Indonesia. Jurnal Ilmu Pertanian Indonesia, 25(2). https://doi.org/10.18343/jipi.25.2.239