GC-MS Analysis of Kabau (Archidendron jiringoides) Pod Peel Extract and Its Effect on Erythrocytes and Leukocytes in Male Mice Induced by Lead

Elisa Nurma Riana; Shafira Aulia; Jeane Siswitasari Mulyana; Iffa Afiqa Khairani; Tirta Setiawan; Lisana Husna Imaniar; Rizka Fatriani; Hida Arliani Nur Anisa

doi:10.18343/jipi.31.4.785

Authors

Elisa Nurma Riana Department of Biology, Faculty of Sciences, Institute of Technology Sumatera, Lampung 35365, Indonesia
Shafira Aulia Department of Biology, Faculty of Sciences, Institute of Technology Sumatera, Lampung 35365, Indonesia
Jeane Siswitasari Mulyana Department of Biology, Faculty of Sciences, Institute of Technology Sumatera, Lampung 35365, Indonesia
Iffa Afiqa Khairani Department of Biology, Faculty of Sciences, Institute of Technology Sumatera, Lampung 35365, Indonesia
Tirta Setiawan Department of Data Science, Faculty of Sciences, Institute of Technology Sumatera, Lampung 35365, Indonesia
Lisana Husna Imaniar Department of Biology, Faculty of Sciences, Institute of Technology Sumatera, Lampung 35365, Indonesia
Rizka Fatriani Department of Biology, Faculty of Sciences, Institute of Technology Sumatera, Lampung 35365, Indonesia
Hida Arliani Nur Anisa Department of Biology, Faculty of Sciences, Institute of Technology Sumatera, Lampung 35365, Indonesia

DOI:

https://doi.org/10.18343/jipi.31.4.785

Keywords:

antioxidant, archidendron, blood cell, GC-MS, kabau

Abstract

An increase in free radicals in the body can result from continuous exposure to heavy metals such as lead (Pb). This condition can result in oxidative stress due to damage to cellular structures, including the lipid bilayer membrane, cellular proteins, and DNA. One approach to reduce such damage is the administration of exogenous plant-derived antioxidants, including kabau. This study aimed to determine the chemical compound profile contained in kabau pod peel extract and to evaluate its effects on erythrocytes and leukocytes in lead-induced mice. GC-MS analysis was conducted to identify the types of compounds present in the extract. An in vivo experiment was conducted with six groups: three control and three treatment groups. The GC-MS results showed that the abundant antioxidant compounds were 2,3-butanediol and 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl. The in vivo results indicated that administration of kabau pod peel extract did not significantly affect leukocyte and erythrocyte counts in the blood of mice exposed to lead acetate.

Keywords: antioxidant, archidendron, blood cell, GC-MS, kabau

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