Isolation and Characterization of Propoxur-Degrading Bacteria, Brucella pseudintermedia LED 6 from a Pineapple Plantation in Lampung

Taufiq Hidayat; Rahayu  Widyastuti; Gunawan  Djajakirana; Sarjiya  Antonius; Tirta Kumala  Dewi

doi:10.18343/jipi.30.4.725

Authors

Taufiq Hidayat Department of Soil Science and Land Resources, Faculty of Agriculture, IPB University, IPB Darmaga Campus, Bogor 16680
Rahayu Widyastuti Department of Soil Science and Land Resources, Faculty of Agriculture, IPB University, IPB Darmaga Campus, Bogor 16680
Gunawan Djajakirana Department of Soil Science and Land Resources, Faculty of Agriculture, IPB University, IPB Darmaga Campus, Bogor 16680
Sarjiya Antonius Microbiology Division, Research Center for Biology, National Research and Innovation Agency (BRIN), Cibinong 16911
Tirta Kumala Dewi Microbiology Division, Research Center for Biology, National Research and Innovation Agency (BRIN), Cibinong 16911

DOI:

https://doi.org/10.18343/jipi.30.4.725

Abstract

Propoxur is a non-systemic carbamate insecticide that is widely used in Indonesia to control insect pests. However, its persistence and toxicity pose environmental concerns. Bioremediation with bacteria is a viable method for mitigating the detrimental effects of propoxur residues. The goal of this work was to isolate and characterize bacterial strains that can degrade propoxur. Isolation by enrichment culture procedures, utilizing propoxur as the sole carbon source. The isolates' morphological and physiological features were examined, and their degradation potential was determined. Six bacterial samples were isolated from a pineapple plantation in Lampung, Indonesia, and one strain, known as LED 6, showed great potential for propoxur breakdown. Molecular identification with 16S rRNA gene sequencing identified the isolate as Brucella pseudintermedia. Growth characterisation revealed that the isolate performed best at 28 °C and pH 7. After 72 hours of incubation with 500 ppm propoxur, LED 6 had deteriorated around 26% of the starting concentration.

Keywords: bioremediation, Brucella pseudintermedia, pineapple, propoxur

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