Perbanyakan In Vitro Sansevieria trifasciata ‘Lorentii’: Regenerasi Tunas, Pengakaran, dan Aklimatisasi Planlet

, Yusnita, Triani Wahyuningsih, Puji Sulistiana, Dwi Hapsoro


This research aimed to study effects of benzyladenine (BA) on in vitro shoot formation and effects of indolebutyric acid
(IBA) and acclimatization media on ex vitro rooting and acclimatization of Sansevieria trifasciata ‘Lorentii’. Leaf segments
were taken from young fully-expanded leaves, surface sterilized and cultured on Murashige and Skoog (MS) basal medium
containing 0.25 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) for 2 weeks, transferred to medium without growth regulator
for 2 weeks, and then subcultured on MS medium containing BA (0, 0.5, 1 and 2 and 5 mg L-1). The results showed that
adventitious shoot regeneration occured after callus formation. The best BA concentration was 2 mg L-1, producing 4.5 shoots
per explants in 3 months and 11.1 shoots per explant in 4 months. Application of 2000 ppm IBA and the use of acclimatization  medium consisting rice husk charcoal and compost (1:1) produced the highest number of primary roots, length of roots and root fresh weight. However, the ex vitro rooting did not influence the success of plantlet acclimatization, the survival rate being 96% and there were no significant difference in plant growth.
Keywords: benzyladenine, ex vitro, indolebutyric acid, 2,4-dichlorophenoxyacetic acid, micropropagation, Sansevieria

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