Kultur Antera untuk Mendapatkan Galur Padi Toleran Salinitas

  • Heni Safitri Indonesian Center for Rice Research
  • Bambang Sapta Purwoko Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
  • Iswari Saraswati Dewi Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian Jl. Tentara Pelajar No. 3A Cimanggu, Bogor 16111, Indonesia
  • dan Sintho Wahyuning Ardie Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Keywords: double haploid, green planlets, indica rice, salt tolerance

Abstract

ABSTRACT

Haploid breeding through anther culture allows shortening of the breeding cycle and production of homozygous lines from a segregating population in the immediate generation. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of twelve F1s, derived from reciprocal crossing between high yielding rice variety and rice tolerance to salinity, through anther culture. Completely randomized design with 20 replications was used in this research. Medium for callus induction was based on N6 medium + 2.0 mg NAA L-1 + 0.5 mg kinetin L-1 + 1 mM putrescine, while regeneration medium was based on MS + 0.5 mg NAA L-1 + 2.0 mg kinetin L-1 + 1 mM putrescine. Rooting were done in MS medium + 0.5 mg IBA L-1 + 1 mM putrescine. The result indicated that F1 derived from IR77674/Inpari 29 (3.1% green plants/total anther) was the most responsive genotypes in rice anther culture (high anther culture ability). After greenhouse grow out 125 putative double haploid plants were obtained (41.5% from total acclimated green plantlets).  

Keywords: double haploid, green planlets, indica rice, salt tolerance

Author Biography

Heni Safitri, Indonesian Center for Rice Research
Plant Breeding
Published
2017-01-19