PENGARUH PEMBERIAN TEPUNG KEDELAI KAYA ISOFLAVON TERHADAP KADAR MALONALDEHID (MDA), AKTIVITAS SUPEROKSIDA DISMUTASE (SOD) TESTIS DAN PROFIL Cu,Zn-SOD TUBULI SEMINIFERI TESTIS TIKUS JANTAN [The effects of isoflavone-riched soybean flour on MDA Level, Sup

  • Sussi Astuti
  • Deddy Muchtadi
  • Made Astawan
  • Bambang Purwantara
  • Tutik Wresdiyati
Keywords: isoflavone - riched soybean flour, lipid peroxide, SOD activity, immunohistochemistry

Abstract

The objectives of this experiment were to evaluate the effects of isoflavone-riched soybean flour with different treatment level of isoflavone on MDA level, SOD activity, and profile of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) spermatogenic cells in the seminiferous tubules of male rat testes. Diet was given as isonitrogen and isocaloric with 10% of dietary protein. Twenty five males of Sprague Dawley rats were divided into five groups and treated with isoflavone-riched soybean flour by oral administration with different concentrations. The treatment was done for 2 months. The optimum concentration of isoflavone was 1.5 mg/day and resulted in : decreased level of MDA testes (2.29±0.05 nmol/g), mantained high SOD activity of rat testes (729.4±23.73 U/g), and increased content of Cu,Zn-SOD by immunohistochemical technique on spermatocytes and early spermatids. It was concluded that isoflavone-riched soybean flour diet (1.5 mg/day isoflavone) can prevent the formation of lipid peroxide and improve the antioxidant status of rat testes.

Author Biographies

Sussi Astuti
Jurusan Teknologi Industri Pertanian, Fakultas Pertanian, Universitas Lampung, Jl. Prof Sumantri Brojonegoro No. 1 Bandar Lampung
Deddy Muchtadi
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian,IPB. PO Box 220, Kampus IPB Darmaga Bogor 16002
Made Astawan
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian,IPB. PO Box 220, Kampus IPB Darmaga Bogor 16002
Bambang Purwantara
Departemen Klinik, Reproduksi, dan Patologi, Fakultas Kedokteran, Hewan, IPB.  Jl. Agatis Kampus IPB Darmaga Bogor 16680
Tutik Wresdiyati
Departemen Anatomi, Fisiologi dan Farmakologi, Fakultas Kedokteran, Hewan, IPB.  Jl. Agatis Kampus IPB Darmaga Bogor 16680
Published
2009-12-12