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  • Memen Surahman
  • . Giyanto
  • Andi Takdir
  • Awaludin Hipi
Keywords: Genetic purity, physiologycal quality, rhizobacteria, phosphate efficiency, seed of maize hybrid


One effort to improve of high-quality of maize seed were the development and application of methods for genetic quality testing, such as SSR marker. Another effort was used of rhizobacteria for increased the availability of nutrients, especially P in the soil. The aim of this study were: 1) Microsatellite markers specific to male and female parents of maize hybrid; 2) Seed genetic purity by using molecular marker; 3) Rhizobacteria that could increase the physiological quality of maize hybrid seed; and 4) Rhizobacteria that could increase the growth of plant and efficiency of fertilizer P. The result showed that: 1) From five markers tested, three markers namely phi96100, phi328175 and phi072 produced polymorphic bands and capable to distinguish parental lines of two maize hybrids. Microsatellite marker phi96100 was specific used for testing genetic purity of cv.Bima-4 and phi072 for cv.Bima-3. While phi328175 was specific markers to both hybrids maize. The test of cv. Bima-3 and Bima-4 indicated that genetic purity of both varieties were 97.5% and 80% respectivelly; 2) Isolates B28 and B46 could increase IV 19% and 22% respectively, and had a high speed of growth compared controls; 3). Isolates B46, B42, B13, P14, P31, AB2, AB3, AB11, ATS4, and ATS5 could increased of germination compared to control; 4) Treatment of rhizobacteri significantly affects on plant height 2, 4 and 6 week after planting (WAP); 5) P fertilizer dosage were not significantly influenced on the number of leaves at 2 and 4 WAP, but significant at 6 WAP. Isolate of B28, B42 and ATS4 were potential for increased of plant growth. 


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Author Biographies

Memen Surahman
Departemen Agronomi dan Holtikultura, Fakultas Pertanian, Institut Pertanian Bogor
. Giyanto
Departemen Proteksi Tanaman Jl. Kamper Kampus IPB Bogor
Andi Takdir
Balai Penelitian Serealia, Jl. Dr. Ratulangi 274 Maros 90514
Awaludin Hipi
Departemen Agronomi dan Holtikultura, Fakultas Pertanian, Institut Pertanian Bogor


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How to Cite
Surahman, M., Giyanto, ., Takdir, A., & Hipi, A. (2012). EVALUASI KEMURNIAN GENETIK DENGAN MARKA MIKROSATELIT DAN APLIKASI RIZOBAKTERIA UNTUK MENINGKATKAN PRODUKSI DAN MUTU BENIH JAGUNG HIBRIDA. Jurnal Ilmu Pertanian Indonesia, 17(1), 22-34. Retrieved from http://journal.ipb.ac.id/index.php/JIPI/article/view/8034

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