Identifikasi gen aroma pada progeni-progeni backcross antara varietas Ciherang dengan Pandan Wangi

  • Djarot Sasongko Hami Seno
  • Akhmad Endang Zainal Hasan
  • Tri Joko Santoso
  • Bram Kusbiantoro
  • Zainal Alim Mas'ud
Keywords: Backcross, Bradbury, fragrant, Mentik Wangi, badh2, site-directed crossing

Abstract

Marker-assisted PCR has been considered as the most potential method for fragrant selection. RM223 is the only suitable marker to identify mutated badh2 gene of Pandan Wangi. This research applies RM223-assisted PCR in the introgression of fragrant gene (mutated badh2) of Pandan Wangi variety, to engineer non-transgenic fragrant variety with good agronomic traits as those of Ciherang.  Gene introduction was carried out through site-directed crossing; Pandan Wangi was crossed and backcrossed to Ciherang until heterozygot BC5F1, followed by selfing to obtain homozygot BC5F2.  RM223-assisted selection was conducted in each cross and backcross generation. RM223 was able to identify native, mutated and heterozygot badh2 of Ciherang, Pandan Wangi, and their cross/backcross progenies, respectively. Therefore, the introgression of mutated badh2 within progenies were observed, as well as the statues of badh2 gene (native/mutated) and alleles (homozygot/heterozygot). Further backcross and selfing to obtain BC5F2 is in progress.

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Author Biographies

Djarot Sasongko Hami Seno
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Akhmad Endang Zainal Hasan
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Tri Joko Santoso
Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian
Bram Kusbiantoro
Balai Besar Penelitian Tanaman Padi
Zainal Alim Mas'ud
LT. Institut Pertanian Bogor

References

Ahn SN, Bollisch CN, Tanksley SD (1992) RFLP tagging of a gene for aroma in rice. Theor Appl Genet 84:825–828.

Amarawathi Y, Singh R, Singh AK, Singh VP, Mohapatra T, Sharma TR, Singh NK (2008) Mapping of quantitative traitloci for basmati quality traits in rice (Oryza sativa L.). Mol. Breed 21:49–65. doi:10.1007/s11032-007-9108-8

Bourgis, F, R. Guyot, H. Gherbi, E. Tailliez, I. Amabile, J. Salse, M. Lorieux, M. Delseny, and A. Ghesquière (2008) Characterization of the major fragance gene from an aromatic japonica rice and analysis of its diversity in Asian cultivated rice. Theor Appl Genet. 117(3): 353–368.

Bradbury LM, Fitgerald TL, Henry RJ, Jin Q, Waters DLE (2005a) The gene for fragrance in rice. Plant Biotech J 3:363–370.

Bradbury LMT, Henry RJ, Jin Q, Reinke RF, Waters DLE (2005b) A perfect marka for fragrance genotyping in rice. Mol Breed 16:279–283.

Buttery RG, Ling LC, Juliano BO, Turnbaugh JG (1983) Cooked rice aroma and 2-acetyl–1-pyroline in rice. J Agric Food Chem 31:823–826.

Cordeiro GM, Christopher MJ, Henry RJ and Reinke RF (2002) Identification of microsatellite markas for fragrance in nrice by analysis of the rice genome sequence. Mol. Breed. 9: 245–250.

Doyle J J and Doyle J L (1990) A rapid total DNA preparation procedure for fresh plant tissue. Focus 12:13-15.

Fitzgerald M A, Hamilton N R S, Calingacion M N, Verhoeven H A, and Butardo, V M (2008) Is there a second fragrance gene in rice. Plant Biotech. J. 6:416-423.

Hami Seno DS, Santoso TJ, Tri Jatmiko KR, Padmadi B, Praptiwi D (2009) Konstruksi padi nonaromatik yang beraroma wangi menggunakan PCR berbantuan marka gen badh2. Prosiding Seminar Hasil-Hasil Penelitian IPB 2009, 678-688. ISBN : 978-602-8853-03-3, 978-602-8853-08-8.

Hami Seno DS, Santoso TJ, Mas’ud ZA (2010) Introgresi aroma padi mentik wangi berbatuan marka bradbury. Prosiding Seminar Hasil-Hasil Penelitian IPB 2010, in press.

Kovach M J, Calingacion M N, Fitzgerald M A, and McCouch S R (2009) The orign and evolution of fragrance in rice (Oriza sativa L.). PNAS 106:14444-14449.

IRRI (2004) Varietas unggul padi sawah yang dilepas sejak 1943-2004. http:www.knowledgebank. irri.orgregionalSitesindonesiadocspadiSawah. Pdf

Kuo SM, Chou SY, Wang AZ, Tseng TH, Chueh FS, Yen HE, Wang CS (2005) The betaine aldehyde dehydrogenase (BAD2) gene is not responsible for aroma trait of AS0420 rice mutant derived by sodium azide mutagenesis. In: Proceedings of the 5th international rice genetics symposium, IRRI, Philippines, p 166.

Lang NT dan Bu BC (2008) Development of pcr-based markas for aroma (fgr) gene in rice (Oryza sativaI L.). Omonrice 16: 16-23.

Lorieux M, Petrov M, Huang N, Guiderdoni E, Ghesquière A (1996) Aroma in rice: genetic analysis of a quantitative trait. Theor App Genet 93:1145–1151.

Mackill DJ, Septiningsih E, Pamploma AM, Sanches D, Iftekhar A, Masudussaman AS, Collard B, Neeraja C, Vergara G, Maghirang-Rodriquez, R, Heuer S, Ismail AM (2007) Marker assisted selection for submergence tolerance in rice. Mol. Plant Breeding 5: 207-208.

Navarro M, Butardo V, Bounphanousay C, Reano R, Hamilton RS, Verhoeven H, Fitzgerald M (2007) The good, the BAD and the fragrant-understanding fragrance in rice. In: Proceedings of international network on quality rices-clearing old hurdles with new science: improving rice grain quality’’, IRRI, Philippines, Apr 17–19, pp 16–17.

Paule CM, Powers JJ (1989) Sensory and chemical examination of aromatic and non aromatic rices. J Food Sci 54:343–346.

Petrov M, Danzart M, Giampaoli P, Faure J, Richard H (1996) Rice aroma analysis Discrimination between a scented and a non scented rice. Sci Aliments 16:347–360.

Reinke RF, Welsh LA, Reece JE, Lewin LG and Blakeney AB (1991) Procedures for quality selection of aromatic rice varieties. Int. Rice Res. Newslett. 16: 10–11.

Sakthivel K, Rani NS, Pandey MK, Sivaranjani AKP, Neeraja CN, Balachandran SM, Madhav MS, Viraktamath BC, Prasad SV, and Sundaram RM (2009) Development of a simple functional marka for fragrance in rice and its validation in Indian Basmati and non-Basmati fragrant rice varieties. Mol. Breeding DOI 10.1007/s11032-009-9283-x

Sambrook J, Fritsch E F, and Maniatis T (1989) Molecular Cloning. A Laboratory Manual. 2nd ed., Books:1-3, Cold Spring Harbor Laboratory Press, USA.

Shi W, Yang Y, Chen S, Xu M (2008) Discovery of a new fragrance allele and the development of functional markas for the breeding of fragrant rice varieties. Mol. Breeding 22: 185-192.

Shure, M, S. Wessler, and N. Fedorrof (1983) Molecular identification and isolation of the Waxy locus in maize. Cell 35: 225-233.

Soedyanto R, Sianipar R, Susani A, dan Harjanto (1978) Bercocok Tanam Jilid II. Jakarta: CV Yasaguna.

Sood BC and Sidiq EA (1978) A rapid technique for scent determination in rice. Indian J. Genetic Plant Breed. 38: 268–271.

Srivong P, Wangsomnuk P and Pongdontri P (2008) Characterization of a fragrant gene and enzymatic activity of betaine aldehyde dehydrogenase in aromatic and nonaromatic thai rice cultivars. KKU Sci. J. 36(4): 290-301.

Sun SH, Gao FY, Lu XJ, Wu XJ, Wang XD, Ren GJ, Luo H (2008) Genetic analysis and gene fine mapping of aroma in rice (Oryza sativa L. Cyperales, Poaceae). Genet Mol Biol 31:532–538. doi:10.1590/S1415-47572008000300021

Tanchotikul U and Hsieh TCY (1991) An improved method for quantification of 2-acetyl-1-pyrroline, a "popcorn`-like aroma, in aromatic rice by high-resolution gas chromatography/mass spectrophotometry/ selective ion monitoring. J. Agric. Food Chem. 39: 944-947.

Vanavichit A, Tragoonrung S, Toojinda T, Wanchana S, and Kamolsukyunyong W (2008) Transgenic rice plants with reduced expression of Os2AP and elevated levels of 2-acetyl-1-pyrroline. USA patent 7,319,181.

Wanchana S, Kamolsukyunyong W, Ruengphayak S, Toojinda T, Tragoonrung S, Vanavichit A (2004) Enhancing 2-acetyl-1-pyrroline synthesis in rice leaves by RNAi-mediated suppression of Os2AP converts non-aromatic to aromatic rice (Oryza sativa L.) Proceedings of the 1.sup.st International Conference on Rice for the Future, p. 105.

Widjaja R, Craske JD. and Wootton M (1996) Comparative studies on volatile components of non-fragrant and fragrant rices. J. Sci. Food Agric. 70: 151–161. (355 bp dan 585 bp)

Yoshihashi T, Huong NTT, and Inatomi H (2002) Precursors of 2-acetyl-1-pyrroline, a potent flavour compound of an aromatic rice variety. J Agric Food Chem 50:2001–2004.

How to Cite
SenoD. S. H., HasanA. E. Z., SantosoT. J., KusbiantoroB., & Mas’udZ. A. (1). Identifikasi gen aroma pada progeni-progeni backcross antara varietas Ciherang dengan Pandan Wangi. Jurnal Ilmu Pertanian Indonesia, 16(2), 136-141. Retrieved from https://journal.ipb.ac.id/index.php/JIPI/article/view/6611

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