Hubunagn kemampuan pergantian inang dengan plastisitas genetika pada cendawan blas padi (Pyricularia grisea)
The Digitaria ciliaris, wild grass grown around rice field, was a host for Pyricularia grisea (Cooke) Sacc., the fungi caused blast disease of rice. This fungi have a specific mechanism to regenerate new genetic variation in its life cycle. The aim of this research is to study the relation between the ability of the fungi to infect different species of host with its genetic plasticity. It was used three SCAR molecular markers Cutl, Pwl 1 and Erg2. P. grisea isolates (Dc4J1) originated from D. ciliaris at Jasinga-Bogor were able to infect rice cultivars Kencana Bali and Cisokan. The original Dc4Jl, from D. ciliaris, and the Dc4Jl that were reisolated from the infected rice cultivars (reisolates-1) had the same ability to infect Kencana Bali and Cisokan. Molecular technique showed that there was a different molecular marker genotype between the original Dc4J1, from D. ciliaris, and the Dc4Jl reisolated from infected rice cultivars. The original Dc4J1 owned Cutl but did not Pwl2 in contrary the reisolates Dc4J1 from rice cultivars (reisolates-1) had Pwl2 but did not Cutl. The Erg2 presented in both the original and the reisolated Dc4Jl. These results indicated that there were a change of genotype of P. grisea at the same time with the change of host species. The Dc4Jl isolates originated from Kencana Bali and Cisokan (reisolates-2) that were infected by reisolate-1, had the same genotype with the reisolates-1.
Babujee, L., Gnanamanickam, S.S. 2000. Molecular tools for characterization of rice blast pathogen (Magnaporthe grisea) population and molecular marker-assisted breeding for disease resistance. Current Science 78: 248-257.
Borromeo, E.S., Nelson, R., Bonman, J.M., Leuang, H. 1993. Genetic differentiation among isolates of Pyricularia infecting rice and weed host. Phytopathology 83:393-399.
Chao, C.T., Ellingboe, A.H. 1997. Genetic analysis of avirulence/virulence of on isolate of Magnaporthe grisea from a rice field in Texas. Phytopathol 87:71-76.
Kahmann, R.C., Basse. 1997. Signaling and development in pathogenic fungi-new strategies for plant protection. Trends in Plant Science 2: 366-367.
Kang, S., Sweigard, J.A., Valent, B. 1995. The pwl host specificity gene family in the blast fungus Magnaporthe grisea (abstrak). Mol Plant Microbe Interact 8:939-948.
Keon, et al., 1994. Isolation of the ERG2 gene, encoding sterol Δ8 Δ7 isomerase, from the rice blast fungus Magnaporthe grisea and its expression in the maize smut pathogen Ustilago maydis. Curr Genet 25:531-537.
Kerjasama Teknis Indonesia-Jepang Bidang Perlindungan Tanaman Pangan (ATA-162). 1992. Penyakit Padi: Laporan Akhir, Tulisan Ilmiah. Jakarta: Direktorat Bina Perlindungan Tanaman, Deptan.
Kurnianingsih, R. 2008. Ekspresi gen PR1 dan PB21 yang terlibat dalam sistem toleransi tanaman padi indica terhadap penyakit blas (isolat 173) [tesis]. Bogor: Program Pascasarjana, Institut Pertanian Bogor.
Mogi, Shizuo, Zaenuddin, S., Wibowo, B.S., Ros, E., Irwan, C. 1991. Establisment of the differential variety series for pathogenic rase identification of rice blast fungus and the distribution of race based on the new differentials in Indonesia. Jatisari: Rice Disease Study Group, Deptan.
Moore-Landecker, E. 1996. Fundamental of Fungi. 4th edition. New Jersey: Prentice Hall, Upper saddle River.
Mysyakina, S., Funtikova, N.S. 2007. The role of sterols in morphogenetic processes and dimorphism in fungi (abstrak). Microbiology 76: 1-13.
Namai, T., and Iwade, Y. 2002. Rice blast fungus induced its pathogenic variation and its gene diversity during proliferation on the resistant rice cultivars (abstract). Di dalam: Abstracts 3rd International Rice Blast Conference; Ibaraki, 11-14 Sept 2002. Japan: Tsukuba International Congress Center-Epochal Tsukuba-Tsukuba Science City. hlm 15. abstr no 28.
Ou, S.H. 1985. Rice diseases. 2nded. England: Commonwealth Mycological Institut, CAB.
Raeder, U., Broda, P. 1985. Rapid preparation of DNA from filamentous fungi. Letters in Apllied Mycrobiology 1:17-20.
Shen et al., 2002. Distribution of the mating type alleles and female fertility in Chinese Magnaporthe grisea populations pathogenic on rice (abstract). Di dalam: Abstracts 3rd International Rice Blast Conference. Ibaraki, 11-14 Sept 2002. Japan: Tsukuba International Congress Center-Epochal Tsukkuba-Tsukuba Science City. hlm 58. abstr no 104.
Singh, N.I., Singh, K.U. 1988. Unrecorded weed host for Pyricularia oryzae Cav. In India. International Rice Research Newsletter 13:31-32.
Soubabere, Q., Jorge, V., Notteghem, J.L., Lebrun, M.H., Tharreau, D. 2001. Sequence characterized amplified region marker for the rice blast fungus, Magnaphorthe grisea. Molecular Ecology Notes1.000-000. Blackwell Science, Ltd. 3p.
Sweigard, J.A., Carrol, A.M., Kang, S., Farrall, L., Chumley, F.G., Valent, B. 1995. Identification, cloning, and characterization of pwl2, a gene for host species-specificity in the rice blast fungus. Plant Cell 7:1221-1233
Sweigard, J.A., Chumley, F.G., Valent, B. 1992a. Cloning and analysis of cut1, a cutinase gene from Magnaporthe grisea (abstrak). Mol Gen Genet 232: 174-182.
Sweigard, J.A., Chumley, F.G., Valent, B. 1992b. Disrupttion of a Magnaporthe grisea cutinase gene (abstrak). Mol Gen Genet 232: 183-190.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).