Evaluation of Inferior Quality Pellets on the Growth Performance, Carcass Parameters, and Digestive Organs of Broiler Chickens
Abstract
Feed form can affect broiler performance. However, it has not been conclusively demonstrated whether the improved growth performance is due to processing (conditioning or pelleting), the physical form of the feed, or a combination of these factors. This study was conducted to investigate the effects of feed form on the growth performance, carcass and cut yields, small intestinal segment lengths, and weights and ratios of several visceral organs in broiler chickens. A total of 450 14-day-old male Ross 308 broilers were weighed and randomly allocated to five treatments, each with nine replicates, using a randomized complete block design. Experimental groups were as follows: 1, mash; 2, conditioned mash; 3, pellet (inferior quality, characterized by low physical integrity; pellet durability index = 51%); 4, the same pellet feed offered at a level equal to the daily mash feed intake of treatment 1; and 5, ground pellet feed. Broilers were fed the same diet formula from 14 to 40 days of age. The results revealed that pellet feed increased feed intake and body weight gain, as well as improved the feed conversion ratio (p ≤0.05). Broilers fed pellet feed at a level equal to the mash feed intake had the highest carcass yield (p ≤0.05). The highest relative weight of the gizzard was observed in broilers fed mash feed (p ≤0.05). The relative lengths of small intestinal segments were reduced in broilers fed pellet feed (p ≤0.05). Based on these results, it can be concluded that the superior performance of pellet feed is mainly attributable to the increased feed intake associated with the pellet form, rather than to modifications induced by thermal processing.
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References
Abadi, M. H. M. G., Moravej, H., Shivazad, M., Torshizi, M. A. K., & Kim, W. K. (2019). Effects of feed form and particle size, and pellet binder on performance, digestive tract parameters, intestinal morphology, and cecal microflora populations in broilers. Poultry Science, 98(3), 1432-1440. https://doi.org/10.3382/ps/pey488
Abdollahi, M. R., Ravindran, V., & Svihus, B. (2013). Influence of grain type and feed form on performance, apparent metabolisable energy and ileal digestibility of nitrogen, starch, fat, calcium and phosphorus in broiler starters. Animal Feed Science and Technology, 186(3–4), 193–203. https://doi.org/10.1016/j.anifeedsci.2013.10.015
Abdollahi, M. R., Ravindran, V., & Svihus, B. (2014). Influence of feed form on growth performance, ileal nutrient digestibility, and energy utilisation in broiler starters fed a sorghum-based diet. Livestock Science, 165, 80–86. https://doi.org/10.1016/j.livsci.2014.04.002
Abdollahi, M. R., Ravindran, V., Wester, T. J., Ravindran, G., & Thomas, D. V. (2011). Influence of feed form and conditioning temperature on performance, apparent metabolisable energy and ileal digestibility of starch and nitrogen in broiler starters fed wheat-based diet. Animal Feed Science and Technology, 168(1–2), 88–99. https://doi.org/10.1016/j.anifeedsci.2011.03.014
Abdollahi, M. R., Zaefarian, F., & Ravindran, V. (2018). Feed intake response of broilers: Impact of feed processing. Animal Feed Science and Technology, 237, 154-165. https://doi.org/10.1016/j.anifeedsci.2018.01.013
AOAC. (2005). Official methods of analysis (18th ed.). Association of Official Analytical Chemists.
ASAE. (1995). Method of determining and expressing fineness of feed materials by sieving. In Agriculture Engineers Yearbook of Standards (pp. 461–462). American Society of Agriculture Engineers Standard S319.2.
ASAE. (2016). Densified products for bulk handling — Definitions and method. ASAE Standard ASAE S269.5.
Attar, A., Kermanshahi, H., Golian, A., Abbasi Pour, A., & Daneshmand, A. (2019). Conditioning time and sodium bentonite affect pellet quality, growth performance, nutrient retention and intestinal morphology of growing broiler chickens. British Poultry Science, 60(6), 777-783. https://doi.org/10.1080/00071668.2019.1663493
Aviagen. (2019). Ross 308 broiler nutrition specification. Retrieved December 10, 2021, from http://en.aviagen.com/assets/Tech_ Center/Ross_Broiler/RossBroilerNutritionSpecs2019-EN.pdf.
Aviagen. (2025). Ross Broiler Management Handbook. Retrieved November 15, 2025, from https://aviagen.com/assets/Tech_Center/Ross_Broiler/Aviagen-ROSS-Broiler-Handbook-EN.pdf
Bordin, T., Pilotto, F., Pesenatto, D., de Mendonça, B. S., Daroit, L., Rodrigues, L. B., Dos Santos, E. D., & Dickel, E. L. (2021). Performance of broiler chicken submitted to a quantitative feed restriction program. Tropical Animal Health and Production, 53(1), 87. https://doi.org/10.1007/s11250-020-02456-7
Boroojeni, F. G., Svihus, B., von Reichenbach, H. G., & Zentek, J. (2016). The effects of hydrothermal processing on feed hygiene, nutrient availability, intestinal microbiota and morphology in poultry—A review. Animal Feed Science and Technology, 220, 187-215. https://doi.org/10.1016/j.anifeedsci.2016.07.010
Cai, J., Hu, Q., Lin, H., Zhao, J., Jiao, H., & Wang, X. (2021). Adiponectin/adiponectin receptors mRNA expression profiles in chickens and their response to feed restriction. Poultry Science, 100(12), 101480. https://doi.org/10.1016/j.psj.2021.101480
Córdova-Noboa, H. A., Oviedo-Rondón, E. O., Ortiz, A., Matta, Y., Hoyos, S., Buitrago, G. D., Martinez, J. D., Yanquen, J., Peñuela, L., Sorbara, J. O., & Cowieson, A.J. (2020). Corn drying temperature, particle size, and amylase supplementation influence growth performance, digestive tract development, and nutrient utilization of broilers. Poultry Science, 99(11), 5681-5696. https://doi.org/10.1016/j.psj.2020.07.010
Dersjant-Li, Y., Awati, A., Kromm, C., & Evans, C. (2013). A direct fed microbial containing a combination of three-strain Bacillus sp. as an alternative to feed antibiotic growth promoters in broiler production. Journal of Applied Animal Nutrition, 2, e11. https://doi.org/10.1017/jan.2014.4
Dixon, L. M., Dunn, I. C., Brocklehurst, S., Baker, L., Boswell, T., Caughey, S. D., Sandilands, V., Wilson, P. W., & D’Eath, R. B. (2022). The effects of feed restriction, time of day, and time since feeding on behavioral and physiological indicators of hunger in broiler breeder hens. Poultry Science, 101, 101838. https://doi.org/10.1016/j.psj.2022.101838
Duarte, C. R. A., Vicentini-Paulino, M. L. M., Buratini, J., Castilho, A. C. S., & Pinheiro, D. F. (2011). Messenger ribonucleic acid abundance of intestinal enzymes and transporters in feed-restricted and refed chickens at different ages. Poultry Science, 90(4), 863–868. https://doi.org/10.3382/ps.2010-01015
Ebeid, T. A., Tůmová, E., Al-Homidan, I. H., Ketta, M., & Chodová, D. (2022). Recent advances in the role of feed restriction in poultry productivity: part I-performance, gut development, microbiota and immune response. World’s Poultry Science Journal, 78(4), 971-988. https://doi.org/10.1080/00439339.2022.2097149
Hamungalu, O., Zaefarian, F., Abdollahi, M. R., & Ravindran, V. (2020). Performance response of broilers to feeding pelleted diets is influenced by dietary nutrient density. Animal Feed Science and Technology, 268, 114613. https://doi.org/10.1016/j.anifeedsci.2020.114613
Hosseini, S. M., & Afshar, M. (2017a). Effect of diet form and enzyme supplementation on stress indicators and bone mineralisation in heat-challenged broilers fed wheat-soybean diet. Italian Journal of Animal Science, 16(4), 616–623. https://doi.org/10.1080/1828051X.2017.1321973
Hosseini, S. M., & Afshar, M. (2017b). Effects of feed form and xylanase supplementation on performance and ileal nutrient digestibility of heat-stressed broilers fed wheat-soybean diet. Journal of Applied Animal Research, 45(1), 550–556. https://doi.org/10.1080/09712119.2016.1224765
Iravani, S., Aziz-Aliabadi, F., & Vakili, R. (2024). Feed processing: a review of the impacts of conditioning time and temperature on feed quality and broilers performance. World’s Poultry Science Journal, 80(3), 657-679. https://doi.org/10.1080/00439339.2024.2341276
Lemons, M. E., & Moritz, J. S. (2016). The effect of feeder space access and crumble- or pellet-to-fine ratio on 38-day-old broiler performance. Journal of Applied Poultry Research, 25(1), 12–20. https://doi.org/10.3382/japr/pfv053
Lemons, M. E., McDaniel, C. D., Moritz, J. S., & Wamsley, K. G. S. (2019). Interactive effects of high or low feed form and phase of feeding on performance of Ross × Ross 708 male broilers throughout a 46-day growout. Journal of Applied Poultry Research, 28(3), 616–630. https://doi.org/10.3382/japr/pfz012
Lunedo, R., Furlan, L. R., Fernandez-Alarcon, M. F., Squassoni, G. H., Campos, D. M. B., Perondi, D., & Macari, M. (2019). Intestinal microbiota of broilers submitted to feeding restriction and its relationship to hepatic metabolism and fat mass: Fast-growing strain. Journal of Animal Physiology and Animal Nutrition, 103(4), 1070–1080. https://doi.org/10.1111/jpn.13093
Lv, M. B., Yan, L., Wang, Z. G., An, S., Wu, M. M., & Lv, Z. Z. (2015). Effects of feed form and feed particle size on growth performance, carcass characteristics and digestive tract development of broilers. Animal Nutrition, 1(4), 252–256. https://doi.org/10.1016/j.aninu.2015.06.001
Mabelebele, M., Gous, R. M., O’Neil, H. V. M., & Iji, P. A. (2018). Whole sorghum inclusion and feed form on performance and nutrient digestibility of broiler chickens. Journal of Applied Animal Nutrition, 6, e5. https://doi.org/10.1017/JAN.2018.3
Mahdavi, R., Osmanyan, A. K., Fisinin, V. I., Harsini, S. G., Arkhipova, A. L., Shevyakov, A. N., Kovalchuk, S. N., & Kosovsky, G. Y. (2018). Impact of mash and crumble diets on intestinal amino acids transporters, intestinal morphology and pancreatic enzyme activity of broilers. Journal of Animal Physiology and Animal Nutrition, 102(5), 1266–1273. https://doi.org/10.1111/jpn.12956
Massuquetto, A., Panisson, J. C., Marx, F. O., Surek, D., Krabbe, E. L., & Maiorka, A. (2019). Effect of pelleting and different feeding programs on growth performance, carcass yield, and nutrient digestibility in broiler chickens. Poultry Science, 98(11), 5497–5503. https://doi.org/10.3382/ps/pez176
Massuquetto, A., Panisson, J. C., Schramm, V. G., Surek, D., Krabbe, E. L., & Maiorka, A. (2020). Effects of feed form and energy levels on growth performance, carcass yield and nutrient digestibility in broilers. Animal, 14(6), 1139–1146. https://doi.org/10.1017/S1751731119003331
Naderinejad, S., Zaefarian, F., Abdollahi, M. R., Hassanabadi, A., Kermanshahi, H., & Ravindran, V. (2016). Influence of feed form and particle size on performance, nutrient utilisation, and gastrointestinal tract development and morphometry in broiler starters fed maize-based diets. Animal Feed Science and Technology, 215, 92–104. https://doi.org/10.1016/j.anifeedsci.2016.02.012
Nourmohammadi, R., Khosravinia, H., & Afzali, N. (2018). Effects of feed form and xylanase supplementation on metabolizable energy partitioning in broiler chicken fed wheat‐based diets. Journal of Animal Physiology and Animal Nutrition, 102(6), 1593-1600. https://doi.org/10.1111/jpn.12980
Oliveira, L. M. S., Silva, P. G., Silva, M. R. S., Cordeiro, D. A., Souza, L. P., Minafra, C. S., & Santos, F.R. (2022). Effect of moisture, particle size and thermal processing of feeds on broiler production. Brazilian Journal of Poultry Science, 24(4), eRBCA-2020-1391. https://doi.org/10.1590/1806-9061-2020-1391
Rasool, A., Qaisrani, S. N., Khalique, A., & Hussain, J. (2025). Interactive effects of feed form, and fiber source and levels on production performance, foregut development, and nutrients utilization in broilers. Brazilian Journal of Poultry Science, 27(01), eRBCA-2024-2020. https://doi.org/10.1590/1806-9061-2024-2020
Rueda, M. S., Bonilla, S., de Souza, C., Starkey, J. D., Starkey, C. W., Mejia, L., & Pacheco, W. J. (2024). Evaluation of particle size and feed form on performance, carcass characteristics, nutrient digestibility, and gastrointestinal tract development of broilers at 39 d of age. Poultry Science, 103(3), 103437. https://doi.org/10.1016/j.psj.2024.103437
Sellers, R. B., Tillman, P. B., Moritz, J. S., & Wamsley, K. G. S. (2017). The effects of strain and incremental improvements in feed form on day 28 to 42 male broiler performance. Journal of Applied Poultry Research, 26(2), 192–199. https://doi.org/10.3382/japr/pfw062
Svihus, B., Abdollahi, M. R., Wamsley, K. G. S., Pacheco, W., & Hetland, H. (2025). Structural architecture of pelleted broiler diets: A comprehensive narrative review of key factors for an optimized macro-and microstructure. Poultry Science, 104(9), 105478. https://doi.org/10.1016/j.psj.2025.105478
Thomas, M., & van der Poel, A. F. B. (1996). Physical quality of pelleted animal feed. I. Criteria for pellet quality. Animal Feed Science and Technology, 61(1–4), 89–112. https://doi.org/10.1016/0377-8401(96)00949-2
Vallejo-Sartorius, Irma, Rendon-Sandoval, L., & Gutierrez-Peña, E. (2019). Humidity and comparative analysis of durability index in pellet of balanced foods for birds. Journal Mathematical and Quantitative Methods. 18-23. https://doi.org/10.35429/JMQM.2019.5.3.18.23
Yan, C., Xiao, J., Chen, D., Turner, S. P., Li, Z., Liu, H., Liu, W., Liu, J., Chen, S., & Zhao, X. (2021). Feed restriction induced changes in behavior, corticosterone, and microbial programming in slow-and fast-growing chicken breeds. Animals, 11(1), 141. https://doi.org/10.3390/ani11010141
You, J., Hall, K., Civiero, J., Malpass, M. C., Tulpan, D., & Ellis, J. L. (2025). Evaluating variables affecting Pellet Durability Index (PDI) in pelleted corn-soy-based feeds for swine and poultry: A meta-analysis. Animal Feed Science and Technology, p.116566. https://doi.org/10.1016/j.anifeedsci.2025.116566
Zaefarian, F., Abdollahi, M. R., Cowieson, A., & Ravindran, V. (2019). Avian liver: the forgotten organ. Animals, 9(2), 63. https://doi.org/10.3390/ani9020063
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