Transformasi Genetik Tanaman Kentang (Solanum tuberosum L.) Varietas IPB CP2 dengan Gen OsGERLP

Genetic Transformation of Potato (Solanum tuberosum L.) IPB CP2 Variety with OsGERLP Gene

Abstract

High dissolved aluminum (Al) content in acidic soil can inhibit root elongation, especially in plants sensitive to Al. The OsGERLP gene is known to be an Al tolerance gene in rice, but the role of this gene in other plants is not yet known. Introduction of the OsGERLP gene through Agrobacterium tumefaciens mediation has been conducted and resulted in putative transgenic plants with transformation and regeneration efficiency of 72.62% and 17.24% respectively using leaf explants and 71.3% and 87.65% using internode explants. Transgene integration analysis in transgenic plants has been performed showing that all transgenic clones contain the OsGERLP gene under the control of the 35SCaMV promoter, indicated by the formation of 1500 bp amplicons. Transgenic plant clones have been tested for Al stress using liquid MS medium with different media and pH combinations. In vitro testing showed that transgenic plant clones have higher tolerance compared to non-transgenic plants. Morphological analysis on Al stressed media showed that transgenic plant clones performed better in all parameters including plant height, total root length, number of roots, shoot and root fresh weight, shoot and root dry weight.