Identifikasi Keragaman Genetik Gen Reseptor Hormon Pertumbuhan (GHR|Alu I) pada Sapi Bali

Authors

  • Zulkharnaim Zulkharnaim
  • . Zakaria
  • R R Noor

DOI:

https://doi.org/10.5398/medpet.2010.33.2.81

Keywords:

Bali cattle, GHR|Alu I, genetic diversity, PCR-RFLP, sequencing

Abstract

Growth hormone receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effects on target tissues. The objective of the study was to estimate genetic diversity of the GHR|AluI gene in bali, limousin, simmental and pesisir cattle. Genotyping was performed on 248 animals, including 162 bali, 21 limousin, 17 simmental and 48 pesisir. Single nucleotide polymorphisms (SNP) had been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induced amino acid substitutions Ser/Gly. Genotype frequencies of bali cattle AA (0.988), GG (0.006) and AG (0.006) were evidenced for the GHR AluI monomorphism, but mostly different from limousin GG (0.667), AA (0.238) and AG (0.095), simmental AG (0.529), GG (0.471) and AA (0.000), pesisir AA (0.604), GG (0.375) and AG (0.021) were the evidence of polymorphism. Homozigosity (monomorphism) in bali cattle might be affected by adaptability in extreme environmental conditions such as poor nutrition and improper management practices. It also could be affected by natural selection and phenotype plasticity phenomena.

 

Downloads

Download data is not yet available.

Author Biographies

  • Zulkharnaim Zulkharnaim

    Program Studi Ilmu Produksi dan Teknologi Peternakan, Sekolah Pascasarjana, Institut Pertanian Bogor

  • . Zakaria

     

    Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor
    Jln. Agatis, Kampus IPB Darmaga Bogor 16680 

     

  • R R Noor

    Departemen Ilmu Produksi dan Teknologi Peternakan, Fakultas Peternakan, Institut Pertanian Bogor
    Jln. Agatis, Kampus IPB Darmaga Bogor 16680

Downloads

Published

2010-10-04

Issue

Vol. 33 No. 2 (2010): Media Peternakan

Section

Articles

License

Authors submitting manuscripts should understand and agree that copyright of manuscripts published are held by Media Peternakan. The statement to release the copyright to Media Peternakan is stated in Form A. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.