Impact of Spirulina platensis Extract as an Immunostimulant on the Histopathological Intestinal of Tilapia Fish (Oreochromis niloticus) Infected with Aeromonas salmonicida
DOI:
https://doi.org/10.29244/jvetbiomed.3.2.%25p.Keywords:
Aeromonas salmonicida, intestine health, Spirulina platensisAbstract
Furunculosis in fish results in financial detriment to freshwater aquaculture in Indonesia. Furunculosis, an infectious disease produced by Aeromonas salmonicida, presents a considerable risk to the large-scale farming of both salmonid and non-salmonid species in freshwater environments. This study sought to evaluate the impact of Spirulina platensis extract on the histopatological intestinal health of tilapia infected with Aeromonas salmonicida. Thirty tilapia, each around 10 cm in length, were distributed into five groups. Negative control (P0-) without immersion in Spirulina platensis extract and free from Aeromonas salmonicida infection. Positive control (P0 +) not immersed in Spirulina platensis extract, infected with Aeromonas salmonicida. Tilapia subjected to doses of 400 mg/L (P1), 600 mg/L (P2), and 800 mg/L (P3) The extraction of Spirulina platensis occurred for three hours on the seventh day (first immersion) and the fourteenth day (second immersion), followed by an evaluation of Aeromonas salmonicida infection on the twenty-first day. A necropsy of the fish was performed on day 28. Histopathological analysis revealed that Spirulina platensis extract reduced intestinal villous erosion and inflammatory cell density in the intestines of tilapia. Immunohistochemical staining demonstrated a decrease in the presence of Aeromonas salmonicida within intestinal tissue at a dosage of 800 mg/L. This work indicates that more research is necessary to examine the histological features of the intestines of tilapia infected with Aeromonas salmonicida with those supplemented with Spirulina platensis through feed.
References
[1] Selvaraj V, Sampath K, & Sekar V. 2008. Administration of lipopolysaccharide increases specific and non-specific immune parameters and survival in carp (cyprinus carpio) infected with Aeromonas hydrophila. Journal of Aquaculture. 286: 176-183.
[2] Austin B, & Austin DA. 2007. Aeromonadaceae representatives (Aeromonas salmonicida). in: bacterial fish pathogens: diseases in farmed and wild fish, 4th edition. Praxis Publishing, Chichester. UK : 24−314.
[3] Castro R, Zarra I, & Lamas J. 2004. Water-soluble extracts modulate the respiratory burst activity of turbot phagocytes. Aquaculture. 229: 67-78.
[4] Ramadhan MK, Arimbi & Sarmanu. 2016. Efek Perendaman ekstrak spirulina platensis sebagai imunostimulan terhadap gambaran histopatologi usus ikan gurame (Osphronemus gouramy) yang diinfeksi Aeromonas hydrophila. Veterina Medika. 9(3): 1-6.
[5] Tayag CM, Lin YC, Liou CH, & Chen JC. 2010. Administration of the hot-water extract of spirulina platensis enhanced teh immune response of white shrimp litopenaeus vannamei and its resistance against vibrio agnolyticus. Journal Fish and Shellfish Immunology.
[6] Kozenko R, & Henson RH. 2010. The Study of Spirulina. Effects on the aids virus. cancer and the immune system. Healthy & Natural Journal. 2.
[7] Bintari IG. 2016. Deteksi Aeromonas hydrophila pada ginjal mencit (Mus musculus) dengan teknik Imunohistokimia. Surabaya : Universitas Airlangga. 1-56.
[8] Boajjang G. 1994. Study on effect and mechanism of polysaccharide of spirulina platensis on body immune function improvement. Book of abstracts. Second Asia Pacific Conference on Algal Biotechnology. Page: 24.
[9] Svendsen YS, Dalmo RA, & Bøgwald J. 1999. Tissue localization of Aeromonas salmonicida in Atlantic salmon, Salmo salar L., following experimental challenge. J. Fish Dis. 22: 125–131
[10] Farto R, Milton DL, Bermúdez M, & Nieto T. 2011. Colonization of turbot tissues by virulent and avirulent Aeromonas salmonicida subsp. salmonicida strains during infection. Dis. Aquat. Org. 95: 167–173.
[11] Ibrahem MD, & Ibrahim MA. 2014. The potential Effects of Spirulina platensis (Arthrospira platensis) on tissue protection of niletilapia (oreochromis niloticus) through estimation of P53 level. Cairo Faculty of Veterinary Medicine. Cairo University. page: 133-136.
[12] Winarni ET. 2014. Potensi Spirulina platensis dalam meningkatkan kekebalan tubuh ikan air tawar. Fakultas Biologi Universitas Jenderal Soedirman. Purwokerto.
[13]Priyatna R, Indarjulianto S, & Kurniasih. 2011. Infeksi Aeromonas salmonicida dari berbagai wilayah di indonesia pada ikan mas (Cyprinus carpio). Biota Vol. 16 (2): 287−297.
[14] Gallay P, Heumann D, Roy DL, Barras C, & Glauser MP. 1993. Lipopolysaccharide-binding protein as a major plasma protein responsible for endotoxemic shock. Proceedings of the National Academy of Sciences (USA). 90: 9935-9938.
[15] Simanjuntak SBI. 2002. Histologi organ lymphoid ikan patin jambal (pangasius djambal bleeker) yang diberi imunostimulan spirulina [Thesis]. Program Pascasarjana. Institut Pertanian Bogor. Bogor.
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