Pengaruh Komposisi Media dalam Perbanyakan Protocorm Like Bodies, Pertumbuhan Planlet, dan Aklimatisasi Phalaenopsis amabilis

Authors

  • Erick Raynalta
  • Dewi Sukma

DOI:

https://doi.org/10.29244/jhi.4.3.131-139

Abstract

ABSTRACT

Phalaenopsis  amabilis  orchid  is one   of the most  popular  species  of orchids and  was inaugurated as one of the  national  flower ' Puspa  Pesona'. The purpose  of this study  was to determine the effects of   various  tissue culture   media composition on  Protocorm Like Bodies (PLBs)  multiplication,  growth  of plantlets,  and  acclimatization  of  Phalaenopsis  amabilis. Basic medium  used was  a half strengh of  Murashige and  Skoog (1/2MS)  and  Hyponex with additional  coconut  water,  benzyladeninepurine,  and  chitosan.  In  PLBs multiplication experiments ,   ½  MS  +  15%  coconut  water  medium produces  the  highest   percentage  of survival  rate.  ½  MS  +  15%  coconut water  medium  and  Hyponex  2  g  L-1+  15%  coconut water  +  2.5 ppm chitosan  medium  gave  the best  effect on  the fresh   weight  of  plantlets. The composition of  the culture medium   significantly affect   the fresh   weight and length of   root   on 8  WAP  (week after planting)   in the   acclimatization stage.

Key words: benzyladeninepurine, chitosan, coconut water, Hyponex,  tissue culture

ABSTRAK

Phalaenopsis amabilis  yang dikenal dengan  nama anggrek bulan merupakan salah satu jenisanggrek  yang  paling  populer  dan  dinobatkan  sebagai  salah satu  bunga  nasional  dengan  sebutan ‘Puspa Pesona’.  Tujuan penelitian ini untuk  mengetahui pengaruh berbagai komposisi media kultur jaringan dalam perbanyakan  Protocorm Like Bodies  (PLBs),  pertumbuhan  planlet, dan aklimatisasi Phalaenopsis amabilis. Media dasar yang digunakan adalah setengah konsentrasi media  Murashige dan  Skoog  (1/2MS)  dan  Hyponex dengan  tambahan  air  kelapa,  benziladeninpurin  (BAP),  dan kitosan. Pada percobaan perbanyakan PLBs, media ½ MS + 15% air kelapa menghasilkan persentase hidup tertinggi.  Media ½ MS + 15% air kelapa dan Hyponex  2 g L-1+ 15% AK + 2.5 ppm  kitosanmemberikan  respon  terbaik  terhadap  bobot planlet.  Komposisi  media  kultur  berpengaruh  nyata terhadap bobot segar dan panjang akar pada 8-MST (minggu setelah tanam) dalam tahap aklimatisasi.

Kata kunci: benzylaminopurin, kitosan, air kelapa, Hyponex, kultur jaringan

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Published

2015-08-08

Issue

Vol. 4 No. 3 (2013): Jurnal Hortikultura Indonesia

Section

Articles

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How to Cite

Pengaruh Komposisi Media dalam Perbanyakan Protocorm Like Bodies, Pertumbuhan Planlet, dan Aklimatisasi Phalaenopsis amabilis. (2015). Jurnal Hortikultura Indonesia (JHI), 4(3), 131-139. https://doi.org/10.29244/jhi.4.3.131-139