Induksi Embriogenesis Somatik Dendrobium mussauense melalui Teknik Thin Cell Layer (TCL) dengan Penambahan 2,4-D

Authors

  • Zozy Aneloi Noli Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Jl. Dr. Mohammad Hatta Limau Manis, Padang, Sumatera Barat 25175, Indonesia
  • Suwirmen Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Jl. Dr. Mohammad Hatta Limau Manis, Padang, Sumatera Barat 25175, Indonesia
  • Dinya Khairani Aisa Tumanggor Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Jl. Dr. Mohammad Hatta Limau Manis, Padang, Sumatera Barat 25175, Indonesia

DOI:

https://doi.org/10.29244/jhi.16.3.157-163

Abstract

In vitro culture provides an effective strategy for conserving and mass propagating the endangered orchid Dendrobium mussauense. This study aimed to evaluate the effects of different concentrations of 2.4-D on somatic embryogenesis (SE) induction using transverse thin cell layer (TCL) and non-TCL techniques. A factorial Completely Randomized Design (CRD) was employed, involving two factors: culture technique (TCL and non-TCL) and 2.4-D concentrations (1, 2, 3, and 4 mg L-¹), with four replications for each treatment. Explants were stem segments from 12-month-old D. mussauense plantlets. The highest survival and SE formation rates were observed in non-TCL explants treated with 4 mg L⁻¹ 2.4-D, reaching 75% and 50%, respectively. The earliest shoot and root emergence occurred at 13–14 and 23–25 days, respectively, under the same treatment. SE development in non-TCL explants treated with 2 mg L⁻¹ 2.4-D progressed through globular, scutellar, and coleoptilar stages within two weeks. Overall, 2.4-D concentrations of 2 and 4 mg L-¹ were most effective for SE induction in D. mussauense using both TCL and non-TCL techniques.


Keywords: auxin, explant, SE, root induction, shoot induction.

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Author Biography

  • Zozy Aneloi Noli, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Jl. Dr. Mohammad Hatta Limau Manis, Padang, Sumatera Barat 25175, Indonesia

    Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas,
    Jl. Dr. Mohammad Hatta Limau Manis, Padang, Sumatera Barat 25175, Indonesia

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Published

2025-12-30

Issue

Vol. 16 No. 3 (2025): Jurnal Hortikultura Indonesia (JHI)

Section

Articles

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How to Cite

Induksi Embriogenesis Somatik Dendrobium mussauense melalui Teknik Thin Cell Layer (TCL) dengan Penambahan 2,4-D. (2025). Jurnal Hortikultura Indonesia (JHI), 16(3), 159-163. https://doi.org/10.29244/jhi.16.3.157-163