Pertumbuhan Seedling Anggrek Tanah (Spathoglottis plicata Blume) in Vitro sebagai Respons terhadap Berbagai Komposisi Media MS

In Vitro Seedling Growth of Ground Orchid (Spathoglottis plicata Blume) as a Response to Various MS Media Compositions

Authors

  • Made Ria Defiani Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana Jl. Raya Kampus Unud, Bukit Jimbaran, Kuta Selatan, Badung, Bali 80361, Indonesia
  • Ida Ayu Astarini Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana Jl. Raya Kampus Unud, Bukit Jimbaran, Kuta Selatan, Badung, Bali 80361, Indonesia
  • I Made Saka Wijaya Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana Jl. Raya Kampus Unud, Bukit Jimbaran, Kuta Selatan, Badung, Bali 80361, Indonesia
  • Ni Luh Putu Kayika Febryanti Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana Jl. Raya Kampus Unud, Bukit Jimbaran, Kuta Selatan, Badung, Bali 80361, Indonesia

DOI:

https://doi.org/10.29244/jhi.15.1.49-54

Abstract

Spathoglottis plicata Blume is a ground orchid which has high economic and aesthetic values. Its existence in natural habitat is classified as vulnerable. Propagation of this orchid in vitro can be done by seed germination and seedling growth, which require a suitable culture medium. Murashige and Skoog (MS) has been widely used for in vitro seed germination and seedling growth of various orchids. However, the concentration of salts in MS often needs to be adjusted for best results. This study aimed to investigate in vitro growth of S. plicata seedlings as a response to various MS modification media. Protocorms of 6 weeks after seed sowing were cultured on MS modification media, namely, ¼ MS, ½ MS, MS0, and MS with addition of NAA and BAP. Treatments were arranged in a completely randomized design with 10 replications, each of which consisted of one culture vessel containing 5 clumps of protocorms. After six months in the treatment media, it was found that MS0 was the best medium for in vitro seedling growth, showing the highest leaf numbers and number of seedlings. After 4 weeks ex vitro, 13% of plantlets survived when acclimatized in a plastic shade house using rice-husk charcoal medium.

Keywords: BAP, NAA, acclimatization, protocorm

Downloads

Download data is not yet available.

Downloads

Published

2024-04-30

Issue

Vol. 15 No. 1 (2024): Jurnal Hortikultura Indonesia (JHI)

Section

Articles

License

You are free to:

  1. Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
  2. Adapt — remix, transform, and build upon the material for any purpose, even commercially.
  3. The licensor cannot revoke these freedoms as long as you follow the license terms.

Under the following terms:

  1. Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  2. ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
  3. No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Notices:

You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.

No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.

How to Cite

Pertumbuhan Seedling Anggrek Tanah (Spathoglottis plicata Blume) in Vitro sebagai Respons terhadap Berbagai Komposisi Media MS: In Vitro Seedling Growth of Ground Orchid (Spathoglottis plicata Blume) as a Response to Various MS Media Compositions. (2024). Jurnal Hortikultura Indonesia (JHI), 15(1), 49-54. https://doi.org/10.29244/jhi.15.1.49-54