Pendugaan Vigor Daya Simpan Benih Kubis (Brassica oleracea L. var. capitata) Menggunakan Metode Pengusangan Cepat dengan Etanol
DOI:
https://doi.org/10.29244/jhi.6.3.152-160Abstract
ABSTRACT
A method that can predict vigor of seed correlated to seed longevity is necessary for cabbage seed production. The objective of this study was to obtain effective soaking time in the chemically accelerated aging method with liquid ethanol 20%, that can estimate vigor correlated to seed longevity of cabbage. Cabbage seed consisted of 6 commercial seed lots with different initial vigor, i.e. G1, MG, GC, GT, B3, and KC. In the first experiment, the seeds were stored for 6 months in aluminum foil at open storage with temperature of 23.3-29.9 0C and 61-85% relative humidity. In the second experiment, rapid aging was done by soaking seeds in liquid ethanol 20% for 30, 60, 90, and 120 minutes. The results showed that vigor index of ethanol soaking for 30 minutes in ethanol 20% had close correlation with germination of seed after storage for 6 months with a coefficient correlation r=0.92. Therefore, it could be used to predict vigor correlated to storability. Cabbage seed vigor after 6 months could be predicted by the equation y=3.338+1.054x, where x was variable vigor index after soaking seeds in ethanol 20% for 30 minutes, and a coefficient of determination R2=0.84.
Keywords: deterioration, rapid aging, seed longevity, seed storage, viability
ABSTRAK
Metode yang dapat menduga vigor daya simpan benih kubis sangat diperlukan untuk mendukung penyediaan benih kubis yang bermutu. Penelitian ini bertujuan untuk mendapatkan waktu perendaman yang efektif pada metode pengusangan cepat kimia dengan etanol 20%, yang dapat menduga vigor daya simpan benih kubis. Benih kubis yang digunakan terdiri atas 6 lot benih komersial, yaitu G1, MG, GC, GT, B3, dan KC dengan vigor awal yang berbeda. Percobaan pertama adalah penyimpanan benih dalam kemasan aluminium foil selama 1 sampai 6 bulan pada ruang simpan terbuka (suhu 23.3-29.9 0C dan RH 61-85%). Percobaan kedua adalah pengusangan dengan merendam benih dalam larutan etanol 20% selama 30, 60, 90, dan 120 menit. Hasil menunjukkan indeks vigor setelah perendaman dalam etanol selama 30 menit memiliki korelasi yang erat dengan daya berkecambah benih setelah disimpan selama enam bulan dengan koefisien korelasi sebesar 0.92, sehingga dapat digunakan untuk menduga vigor daya simpan. Vigor daya simpan benih kubis setelah penyimpanan selama enam bulan dapat diduga dengan persamaan y= 3.338 + 1.054x, dengan x peubah indeks vigor setelah perendaman etanol 20% selama 30 menit, dan koefisien determinasi (R2) sebesar 0.84.
Kata kunci: kemunduran benih, pengusangan cepat, daya simpan benih, penyimpanan benih, viabilitas
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