Jurnal Fitopatologi Indonesia
type: channel
description: Jurnal Fitopatologi Indonesia (JFI) is an Indonesian-based scientific journal, published by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia) and suported by the Department of Plant Protection, Faculty of Agriculture, IPB University. JFI is published 6 times a year (January, March, May, July, September and November). The journal is an open-access and Accredited by Directorate General of Higher Education (DGHE), Republic of Indonesia No. 85/M/KPT/2020, 1 April 2020, as "SINTA 2" of Indonesian journal accreditation level (until volume 20 issue 2 2024). Also, has been registered in Crossref, DOAJ, EBSCO, Google Scholar, Portal Garuda, Dimension, and other scientific databases. JFI includes manuscript articles relating to plant diseases and their control, including pathogenic characterization, pathogen detection and identification, physiology and biochemistry of diseases, molecular biology, morphology and ultrastructure, genetics, disease transmission, ecology and epidemiology, chemical and biological control, also topics about characteristics of biocontrol agents and abiotic abnormalities in plant health. Articles can be either original research papers, short communications, or disease note. P-ISSN: 0215-7950 E-ISSN: 2339-2479 Download: Author Guideline
publisher: The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
language: en
publication name: Jurnal Fitopatologi Indonesia
issn: 0215-7950
copyright: Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms: 1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. 2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal. 3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
items: Cover Jurnal Fitopatologi Vol. 20 No. 6, November 2024
items: Peran Trichoderma spp. sebagai Agen Biokontrol pada Penyakit Rebah Semai dan Biostimulan Kedelai
items: Pengaruh Bakteri Endofit Padi terhadap Penyakit Busuk Bulir Bakteri dan Pertumbuhan Tanaman Padi
items: Keefektifan Tumbuhan Rorippa indica sebagai Biofumigan untuk Pengendalian Nematoda Puru Akar pada Tanaman Mentimun
items: Bakteri Endofit Penghasil AHL-Laktonase Asal Tanaman Pisang untuk Pengendalian Penyakit Darah
items: Status Terkini Penyakit Tristeza Pada Tanaman Jeruk di Sulawesi Tenggara
items: Aplikasi Plant Growth Promoting Rhizobacteria Akar Bambu dan Mikroorganisme Lokal pada Tanaman Kedelai untuk Pengendalian Penyakit Pustul Bakteri (Xanthomonas axonopodis pv. Glycines)
items: Ketahanan Varietas Padi terhadap Nematoda Pucuk Putih Aphelenchoides besseyi
items: Identification of Polerovirus Infecting Chili Pepper in Java
items: Induksi Ketahanan Tanaman Tomat terhadap Ralstonia syzygii subsp. indonesiensis Menggunakan Bakteri Endofit dan Asam Salisilat
items: Cover Jurnal Fitopatologi Vol. 20 No. 5, September 2024
items: Metode DAS-ELISA dan RT-PCR untuk Deteksi Potyvirus yang Menginfeksi Bawang Merah
items: Aktivitas Antagonisme Cendawan Endofit terhadap Cendawan Penyebab Bintik Mata Cokelat Daun Kopi
items: Bakteri PGPR Rizosfer Bambu Sebagai Agens Biokontrol Penyakit Moler
items: Deteksi Odontoglossum ringspot virus pada Anggrek Asli Koleksi Kebun Raya di Indonesia
items: Keragaman Morfologi, Genetika, dan Patogenisitas Colletotrichum acutatum PenyebabAntraknosa Cabai di Jawa dan Sumatera
items: Mekanisme Pengendalian Penyakit Busuk Batang Jeruk oleh Khamir, Kitosan, Cendawan Mikoriza Arbuskular, dan Bakteri Simbiotiknya
items: Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat
items: Penyakit Karat Bawang Daun yang Disebabkan oleh Puccinia allii Rud.
items: Ketahanan Kultivar Pisang Lokal Kalimantan Selatan terhadap Penyakit Bercak Sigatoka (Mycosphaerella sp.)
items: Kepekaan Erythricium salmonicolor Penyebab Penyakit Jamur Upas pada Kemiri Sunan terhadap Suhu, Cahaya, dan Fungisida
items: Cara Preservasi Fitoplasma dari Jaringan Kacang Tanah Bergejala Sapu untuk Deteksi DNA dengan Teknik PCR
items: Galur Isogenik Xanthomonas oryzae pv. oryzae Hasil Penyinaran Ultraviolet dan Potensinya Sebagai Penginduksi Resistensi Padi terhadap Penyakit Hawar Daun Bakteri
items: Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan
items: Kemampuan Tumbuhan Terna dalam Menekan Potensi Inokulum Rigidoporus microporus
items: Identifikasi Patotipe Xanthomonas oryzae pv. oryzae dari Tanaman Padi di Sulawesi Selatan
items: Deteksi dan Identifikasi Fitoplasma yang Berasosiasi dengan Penyakit Layu Kelapa Di Pulau Derawan, Kalimantan Timur
items: Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara
items: Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro
items: Aplikasi Filtrat Guano terhadap Infeksi Pepper yellow leaf curl virus pada Tanaman Cabai

Cover Jurnal Fitopatologi Vol. 20 No. 6, November 2024
type: item
description: This editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Vol. 20 No. 6, November 2024.
subject: Editorial
  topic: section
subject: -
  topic: keywords
creator: Editors Jurnal Fitopatologi Indonesia
rights: Copyright (c) 2025 https://creativecommons.org/licenses/by-sa/4.0
date: 2024-11-30
publication date: 2024-11-30
starting page: i
ending page: i
doi: 10.14692/jfi.20.6.i

Peran Trichoderma spp. sebagai Agen Biokontrol pada Penyakit Rebah Semai dan Biostimulan Kedelai
type: item
description: The Role of Trichoderma spp. as a Biocontrol Agent of Damping Off Disease and Soybean Biostimulant Domestic demand for soybeans in Indonesia continues to increase each year, yet the average production has declined. To meet national needs, the government must even import soybeans. One of the causes of low soybean production is damage from plant pests and diseases, particularly damping-off caused by Sclerotium rolfsii, which can lead to total plant death under severe infection. One promising, environmentally friendly, and cost-effective approach to disease management is the use of biological control agents such as Trichoderma spp., which function not only as biocontrol agents but also as biostimulants. This research was conducted at the Plant Pest and Disease Laboratory, Faculty of Agriculture Brawijaya University and Central Laboratory of BALITKABI for in vitro experiments and in vivo study located at a greenhouse of Agriculture Experimental Land Brawijaya University, Jatimulyo, Lowokwaru, Malang. A completely randomized design was used with five treatments and five replications. Applications of Trichoderma harzianum and T. asperellum, either individually or in combination, successfully reduced the incidence and severity of damping-off disease by 14% to 26.6% under field conditions. These treatments also increased the total phenolic content of the plants, indicating enhanced resistance, thus supporting the role of Trichoderma as an effective biocontrol agent. In addition, the combined application of Trichoderma species significantly increased the number of soybean leaves, indicating a biostimulant effect.
subject: Articles
  topic: section
subject: biocontrol
  topic: keywords
subject: biostimulant
  topic: keywords
subject: soybean
  topic: keywords
subject: total phenolic compounds
  topic: keywords
subject: Trichoderma
  topic: keywords
creator: Lu'lu'il Maknunin
creator: Abdul Latief Abadi
creator: Fery Abdul Choliq
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia https://creativecommons.org/licenses/by-sa/4.0
date: 2025-12-09
publication date: 2025-12-09
starting page: 305
ending page: 314
doi: 10.14692/jfi.20.6.304-314

Pengaruh Bakteri Endofit Padi terhadap Penyakit Busuk Bulir Bakteri dan Pertumbuhan Tanaman Padi
type: item
description: Effects of Rice Endophytic Bacteria on Bacterial Grain Rot Disease and Rice Growth Bacterial grain rot disease, caused by Burkholderia glumae, is an important disease that can reduce rice yields and is a seed-borne disease. Biological control through biopriming using endophytic bacteria is an alternative disease control method that has been widely used in various crops. This study aimed to assess the potential of endophytic bacteria from six rice varieties using biopriming techniques in controlling B. glumae and their ability to stimulate rice growth. The research stages consisted of isolation and biosafety testing of endophytic bacteria with potential as biological agents, in vitro antagonism assay against B. glumae using the cross streak method, phytotoxicity assay of endophytic bacteria on rice seeds, selection of potential bacteria using the analytical hyphenation process (AHP) method, and examining the ability of biopriming to control B. glumae and its effect on rice vegetative growth. A total of 133 endophytic bacterial isolates were successfully isolated from six rice varieties, and 27 isolates met the biosafety test requirements. In vitro and in vivo assay showed that eight isolates exhibited antagonistic activity against B. glumae, and 25 isolates increased rice seed germination. Three isolates were selected based on AHP results, i.e. BBZN3, BBAR4, and AKPAT4. Of these three isolates, AKPAT4 was the best at increasing the vigor index and germination level of rice seeds, as well as suppressing disease incidence and severity.
subject: Articles
  topic: section
subject: analytical hyrearchy process
  topic: keywords
subject: biopriming
  topic: keywords
subject: Burkholderia glumae
  topic: keywords
subject: rice seed germination
  topic: keywords
creator: Nahrisa Rahmadhani
creator: Abdul Munif
creator: Abdjad Asih Nawangsih
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia https://creativecommons.org/licenses/by-sa/4.0
date: 2025-12-09
publication date: 2025-12-09
starting page: 51
ending page: 63
doi: 10.14692/jfi.21.1.51-63

Keefektifan Tumbuhan Rorippa indica sebagai Biofumigan untuk Pengendalian Nematoda Puru Akar pada Tanaman Mentimun
type: item
description: Effectiveness of Rorippa indica as a Biofumigant for Controlling Root Knot Nematodes on Cucumber Plants Meloidogyne spp. are important plant parasitic nematodes, causing disease symptoms such as root knot, stunting, yellowing of leaves, and wilting. Botanical nematicides have potential as a method for nematode control. This study aimed to determine the potential of Rorippa indica as a biofumigant in controlling root knot nematodes (Meloidogyne incognita). The biofumigation experiment was conducted in two stages, i.e. a laboratory experiment and a greenhouse experiment with a 14-day incubation period to initiate the biofumigation process. The laboratory experiment used polybags containing 100 g of soil infested with 100 juvenile 2 (J2) M. incognita before the biofumigation treatment. Stem fragments, leaves, and all plant parts of R. indica were mixed into the polybags at weights of 1.3, 2.3, and 3.9 g, respectively, as treatments. A greenhouse experiment was conducted by adding 150 g of R. indica cuttings per polybag containing 3 kg of soil infested with 300 J2 of M. incognita. Cucumber plants, 7 days after sowing, were transferred into biofumigated polybags and observed for 6 weeks. The results showed that biofumigation using R. indica on a laboratory scale was effective in reducing nematode populations by 87%. Meanwhile, at the greenhouse scale, it caused a significant difference in gall formation compared to the control treatment. This is related to the results of the GC-MS analysis which showed terpenoid content in the R. indica extract. The R. indica treatment significantly increased cucumber plant growth compared to the control.
subject: Articles
  topic: section
subject: juvenile
  topic: keywords
subject: Meloidogyne incognita
  topic: keywords
subject: nematicides
  topic: keywords
subject: terpenoid
  topic: keywords
creator: Muhammad Willy Bramasta
creator: Supramana
creator: Abdul Munif
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia https://creativecommons.org/licenses/by-sa/4.0
date: 2025-12-02
publication date: 2025-12-02
starting page: 295
ending page: 304
doi: 10.14692/jfi.20.6.295-304

Bakteri Endofit Penghasil AHL-Laktonase Asal Tanaman Pisang untuk Pengendalian Penyakit Darah
type: item
description: Endophytic Bacteria Producing AHL-Lactonase from Banana Plant to Control Blood Disease Blood disease is an important disease in banana that caused 100% yield loss. The Ralstonia solanacearum Phylotype IV as the causal agent for this disease express their virulence genes through quorum sensing (QS) mechanism by using acylated homoserine lactone (AHL) as autoinducer. AHL-lactonase enzyme is produced by bacteria, and its potential as biocontrol agents performed through anti-QS. This research was aimed to obtain endophytic bacteria isolates producing AHL lactonase for controling R. solanacearum in banana plants. The research stages include isolation and selection of endophytic bacteria from banana plant as producer of AHL lactonase by using bioassay and molecular, the effectiveness testing of the bacterial isolates in suppressing R. solanacearum pathogenic virulence factors, in planta testing for controlling R. solanacearum, and molecular identification of potential bacterial isolates. We successfully explored endophytic bacteria from low-, medium-, and up-land consisted several varieties of banana, resulting 152, 204, and 130 isolates, respectively. Further selection using bioindicator showed five isolates with anti quorum sensing activity against Chromobacterium violaceum. Detection of aiiA gene by polymerase chain reaction using specific primer indicated all of five isolates AHL degradation activity resulted 753 bp amplicon that has been identified as aiiA gene. These isolates (CBP1, DBT17, DBT7, DBK2, and CBN13) were able to suppress the formation of exstracellular polysacharide (EPS) produced by R. solanacearum with suppression level of 75.67%, 90.81%, 87.67%, 92.72%, 81.08%, and were able to inhibit wilt symptom in banana plant. Nucleotide sequences of aiiA gene of these five isolates has homology with aiiA genes from Bacillus cereus, B. thuringiensis, B. substilis, B. amyloliquefaciens.
subject: Articles
  topic: section
subject: autoinducer
  topic: keywords
subject: extracellular polysacharide
  topic: keywords
subject: quorum sensing
  topic: keywords
subject: Ralstonia solanacearum
  topic: keywords
subject: virulence gene
  topic: keywords
creator: Anugrawati Abidin
creator: Giyanto
creator: Abdjad Asih Nawangsih
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia https://creativecommons.org/licenses/by-sa/4.0
date: 2025-08-21
publication date: 2025-08-21
starting page: 276
ending page: 285
doi: 10.14692/jfi.20.6.276-285

Status Terkini Penyakit Tristeza Pada Tanaman Jeruk di Sulawesi Tenggara
type: item
description: Current Status of Citrus Tristeza Disease in Southeast Sulawesi Tristeza disease, caused by citrus tristeza virus (CTV), a member of genus Closterovirus, family Closteroviridae, is one of the economically important diseases on citrus worldwide, including Indonesia. To date, information and research on CTV in Indonesia remain limited. This study aims to assess the current status of CTV infection in Indonesia, especially in Southeast Sulawesi Province. Surveys and sample collections were conducted using a purposive sampling method in four regencies of Southeast Sulawesi Province. Virus detection and identification was carried out using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequence analysis. The result of the ELISA showed that all tested samples with various symptoms from four regencies are positively infected with CTV at different virus titers. Furthermore, RT-PCR also successfully detected CTV across the tested samples with DNA amplicon of 630 bp in size. Nucleotide sequencing and phylogenetic tree analysis revealed that Southeast Sulawesi CTV isolates shared the highest similarity (97.9%) with isolates of CTV from India (MT498411.1) and are clustered in one group with isolates from India (MT498411.1), Japan (U56902.1), dan China (MZ692538.1). The results of this study confirmed the presence and spread of CTV in Southeast Sulawesi. The molecular data are expected to be used as the basis data for further molecular epidemiological studies and disease control of tristeza in Indonesia.
subject: Articles
  topic: section
subject: citrus tristeza virus
  topic: keywords
subject: closterovirus
  topic: keywords
subject: DAS-ELISA
  topic: keywords
subject: epidemiology
  topic: keywords
subject: RT-PCR
  topic: keywords
creator: Muhammad Taufik
creator: Sari Nurulita
creator: Dewi Nurhayati Yusuf
creator: Miftahkuhrohmah Miftahkuhrohmah
creator: Muhammad Botek
creator: Gusnawaty HS Gusnawaty HS
creator: Elisa Syarni
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia https://creativecommons.org/licenses/by-sa/4.0
date: 2025-10-30
publication date: 2025-10-30
starting page: 28
ending page: 37
doi: 10.14692/jfi.21.1.28-37

Aplikasi Plant Growth Promoting Rhizobacteria Akar Bambu dan Mikroorganisme Lokal pada Tanaman Kedelai untuk Pengendalian Penyakit Pustul Bakteri (Xanthomonas axonopodis pv. Glycines)
type: item
description: Application of Bamboo Root Plant Growth Promoting Rhizobacteria and Local Microorganisms in Soybean to Control of Bacterial Pustule (Xanthomonas axonopodis pv. Glycines) Xanthomonas axonopodis pv. glycines (Xag) is a pathogenic bacterium that causes pustule disease in soybean plants, leading to significant levels of damage. One of the biological control efforts against this pathogen involves the use of plant growth-promoting rhizobacteria (PGPR) and local microorganisms (MOL). This study aims to determine the effect of PGPR and MOL applications on enhancing plant growth and controlling bacterial pustule disease. The Xag JB12 isolate from the University of Jember was used as the inoculum source. PGPR from bamboo roots and MOL from fruit and vegetable waste were used as antagonistic agents against the Xag JB12 isolate. The experimental study was designed using four treatments (control, PGPR application, MOL application, and PGPR + MOL application). PGPR/MOL application was carried out through seed soaking and drenching. The results showed that the MOL treatment exhibited the lowest disease severity, which was 20.02%. The MOL treatment showed the highest disease progression rate, but the smallest area under the disease progress curve (AUDPC) compared to other treatments. The study also found that all treatments had no significant effect on plant height and leaf number variables.
subject: Articles
  topic: section
subject: AUDPC
  topic: keywords
subject: biological control
  topic: keywords
subject: disease severity
  topic: keywords
subject: seed soaking
  topic: keywords
creator: Irwan Lakani
creator: Jawuh Zakaria
creator: Rosmini
creator: Mutmainah
creator: Jusriadi
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia https://creativecommons.org/licenses/by-sa/4.0
date: 2025-10-17
publication date: 2025-10-17
starting page: 18
ending page: 27
doi: 10.14692/jfi.21.118-27

Ketahanan Varietas Padi terhadap Nematoda Pucuk Putih Aphelenchoides besseyi
type: item
description: The Resistance of Rice Varieties Against White Tip Nematode Aphelenchoides besseyi White tip disease on rice caused by Aphelenchoides besseyi is considered as a new important disease and it had widely distributed in Indonesia. The utilizing of resistant varieties is one of the best methods to control A. besseyi. However, there is no information regarding the resistance of rice varieties to A. besseyi in Indonesia. Nine rice varieties were evaluated for their resistance response to A. besseyi by inoculating 500 nematode per plant. Nematode A. besseyi was infested at the anthesis phase by spraying the nematode suspension onto the panicles followed by observation on panicle length, number of grains, number of full and empty grains, weight of 100 full grains, number of nematodes and expression of the PBZ1 gene of rice. Expression of PBZ1 gene was conducted by one-step reverse transcription-polymerase chain reaction (RT-PCR) using a specific primer PBZ1F and PBZ1R. Based on yield parameters, A. besseyi population, and PBZ1 gene expression, the resistance response of rice varieties to A. besseyi infestation was differentiated into three categories, namely resistant (‘Ciherang’, ‘Utri Merah’, and ‘Utri Rajapan’), tolerant (‘Inpago 7’, ‘IPB 3S’, ‘Prima’, and ‘Situ Bagendit’) and susceptible (‘Inpara 3’ and ‘Inpara 4’). Aphelenchoides besseyi’s infestation induces PBZ1 gene expression in the tested varieties at different intensities depending on their level of resistance. ‘Ciherang’, ‘Inpago 7’, ‘Situ Bagendit’, ‘Utri Merah’, and’ ‘Utri Rajapan’ showed high PBZ1 gene intensity both before and after A. besseyi infestation. The high intensity PBZ1 gene expression before A. besseyi infestation showed that this varieties have a potential source of resistant to A. besseyi. Based on the results there is a correlation between the high expression of PBZ1 gene with the resistance of rice varieties to A. besseyi.
subject: Articles
  topic: section
subject: disease intensity
  topic: keywords
subject: namatode population
  topic: keywords
subject: PBZ1 gene
  topic: keywords
subject: resistance varieties
  topic: keywords
subject: RT-PCR detection
  topic: keywords
creator: Annisa Nur Imamah
creator: Supramana Supramana
creator: Tri Asmira Damayanti
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia
date: 2025-07-04
publication date: 2025-07-04
starting page: 7
ending page: 17
doi: 10.14692/jfi.21.1.7-17

Identification of Polerovirus Infecting Chili Pepper in Java
type: item
description: Infection of polerovirus in chili peppers has been reported from various parts of the world, including Indonesia. Pepper vein yellow virus (PeVYV), a member of genus Polerovirus were reported infecting chili plants in Bali, Java, and Aceh during 2012 to 2020. Recently, symptoms of the polerovirus infection, such as yellowing leaves with vein thickening, were found in chili pepper crops in the Central Java Region, specifically in Brebes, Magelang, and Kulon Progo. Molecular detection by RT-PCR method using universal polerovirus primers O3R/PL4F, followed by sequence analysis, confirmed the presence of PeVYV on samples from Magelang and Kulon Progo. Furthermore, the isolates of PeVYV are identified as PeVYV-2, PeVYV-6, and PeVYV-8. These current incidences indicate the expansion of the Polerovirus distribution in Indonesia.
subject: Short Comunication
  topic: section
subject: Pepper vein yellow virus
  topic: keywords
subject: RT-PCR
  topic: keywords
subject: sequence analysis
  topic: keywords
subject: vein thickening
  topic: keywords
creator: Andri Saputra
creator: Giyanto
creator: Sari Nurulita
creator: Sri Hendrastuti Hidayat
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia
date: 2025-07-03
publication date: 2025-07-03
starting page: 1
ending page: 6
doi: 10.14692/jfi.21.1.1-6

Induksi Ketahanan Tanaman Tomat terhadap Ralstonia syzygii subsp. indonesiensis Menggunakan Bakteri Endofit dan Asam Salisilat
type: item
description: Induction of Resistance in Tomato Plants Against Ralstonia syzygii subsp. indonesiensis Using Endophytic Bacteria and Salicylic Acid Ralstonia syzygii subsp. indonesiensis causes bacterial wilt disease in tomato plants. This pathogen is difficult to control because the pathogen can persist in residual host material, and soil, and easily spread through water and infected plant tissue systemically. There are no chemical pesticides available to control bacterial wilt pathogens. Therefore, the alternative is biological control using endophytic bacteria and salicylic acid. Two endophytic bacteria, namely Arthrobacter sp. and Bacillus thuringiensis, as well as salicylic acid, were used in research singly or in combination to evaluate their potential in inducing tomato plant resistance in suppressing the bacterial wilt disease R. syzygii subsp. indonesiensis. The research uses a non-factorial completely randomized design with 14 single and combination treatments. Each treatment consisted of three experimental units and was repeated three times. The results showed the combination of Arthrobacter sp. and salicylic acid applied once a week (ABAS1) was the most effective treatment in inducing tomato resistance. Evidenced by the longest incubation period, low incidence and disease severity, increased peroxidase, polyphenol oxidase activity, and xylem tissue was not predominantly colonized by pathogens in histopathological observations.
subject: Articles
  topic: section
subject: Arthrobacter sp.
  topic: keywords
subject: Bacillus thuringiensis
  topic: keywords
subject: biological control
  topic: keywords
subject: wilt disease
  topic: keywords
creator: Triwanto Nababan
creator: Lisnawita Lisnawita
creator: Irda Safni
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia
date: 2025-06-13
publication date: 2025-06-13
starting page: 263
ending page: 275
doi: 10.14692/jfi.20.6.263-275

Cover Jurnal Fitopatologi Vol. 20 No. 5, September 2024
type: item
description: This editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Vol. 20 No. 5, September 2024.
subject: Editorial
  topic: section
subject: -
  topic: keywords
creator: Editors Jurnal Fitopatologi Indonesia
rights: Copyright (c) 0
date: 2025-05-21
publication date: 2025-05-21
starting page: i
ending page: i

Metode DAS-ELISA dan RT-PCR untuk Deteksi Potyvirus yang Menginfeksi Bawang Merah
type: item
description: DAS-ELISA and RT-PCR Methods for Detection of Potyviruses Infecting Shallot Viral infection of shallots in Indonesia may reduce yields, mainly because it is bulb-borne. Virus detection to monitor the incidence of disease in the field or examine healthy seeds requires an accurate and reliable method. In this study, the detection of Potyvirus members, i.e., Onion yellow dwarf virus (OYDV) and Shallot yellow stripe virus (SYSV), was conducted using enzyme-linked immunosorbent assay (DAS-ELISA) and reverse transcription polymerase chain reaction (RT-PCR) from shallot leaf samples. Leaf samples were collected from several shallot growing areas in Brebes and Cirebon. Infections of OYDV and SYSV were detected using the DAS-ELISA method, then field samples that gave positive reaction were selected for further detection using the RT-PCR method, followed by nucleotide sequencing. Based on the DAS-ELISA method, OYDV and SYSV infections were detected from field samples as single and mixed infections. Potyvirus infection was confirmed by the RT-PCR method from field samples that gave a positive reaction in ELISA. Nucleotide sequence analysis showed that OYDV infection was detected in both samples that reacted positively with OYDV and SYSV antiserum. These results indicated the cross-reaction between OYDV and SYSV antisera.
subject: Articles
  topic: section
subject: cross-reaction
  topic: keywords
subject: Onion yellow dwarf virus
  topic: keywords
subject: RT-PCR
  topic: keywords
subject: sequence analysis
  topic: keywords
subject: Shallot yellow stripe virus
  topic: keywords
creator: Ivan Arif Rachman Martua Siregar
creator: Sri Hendrastuti Hidayat
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia
date: 2025-05-20
publication date: 2025-05-20
starting page: 255
ending page: 262
doi: 10.14692/jfi.20.5.255-262

Aktivitas Antagonisme Cendawan Endofit terhadap Cendawan Penyebab Bintik Mata Cokelat Daun Kopi
type: item
description: Antagonism Activity of Endophytic Fungi Against Pathogenic Fungus That Cause Coffee Brown Eye Spot Coffee is a high economic value commodity and is a source of foreign exchange for Indonesia. Coffee productivity and quality have decreased due to brown eye spot disease caused by fungi infection. One alternative method to overcome the growth of pathogenic fungi is to use endophytic fungi as an antagonistic biological agent. This study aims to identify the fungi causing coffee plant brown eye spot disease and its antagonistic endophytic fungi, and to evaluate the mechanisms and potential of antagonism. Pathogenic fungi were isolated from coffee leaves showing symptom of brown eye spot disease. Endophytic fungi were isolated from healthy leaves obtained from the Taji Village Coffee Plantation, Jabung District, Malang Regency. Each fungus was cultured and then purified using the monospore method. Pathogenic fungi isolates were selected based on the pathogenicity test. Endophytic fungi isolates were tested for their antagonistic properties against selected brown eye spot pathogenic fungi isolates and the endophytic fungus that had the highest potential to inhibit growth of the pathogenic fungi were identified based on 18S rDNA sequence similarity. The identification results show that the pathogenic fungus has 100% sequence similarity with Mycosphaerella coffeicola-PF2 while the antagonistic endophytic fungus has 99.94% similarity with Colletotrichum sp.-EF1. The endophytic fungi Colletotrichum sp. inhibits the growth of M. coffeicola by competition and mycoparasite mechanisms. The endophytic fungi Colletotrichum sp. can be developed as a biopesticide agent to control M. coffeicola which causes brown eyespot disease on coffee plant leaves.
subject: Articles
  topic: section
subject: Colletotrichum sp.
  topic: keywords
subject: Koch postulate
  topic: keywords
subject: monospore method
  topic: keywords
subject: mycoparasite
  topic: keywords
subject: Mycosphaerella coffeicola
  topic: keywords
creator: Yulia Dwi Asparita
creator: Luqman Qurata Aini
creator: Suharjono
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia
date: 2025-04-08
publication date: 2025-04-08
starting page: 242
ending page: 254
doi: 10.14692/jfi.20.5.242-254

Bakteri PGPR Rizosfer Bambu Sebagai Agens Biokontrol Penyakit Moler
type: item
description: The PGPR Bacteria from Bamboo Rhizosphere as a Biocontrol Agent for Moler Disease Plant growth promoting rhizobacteria are often used as biological agents, either against F. oxysporum or other pathogens. PGPR from bamboo roots have the potential to be used to control Moler disease in shallots. The objective of this study is to evaluate the potential of PGPR from bamboo roots as an antagonist against F. oxysporum. Samples were collected from the rhizosphere of bamboo in different locations in West Kalimantan and isolated using the plating method on nutrient agar. The isolates obtained were tested in vitro as antagonists against F. oxysporum. The results of the test revealed 30 isolates, 19 of which showed inhibition against F. oxysporum. Seven isolates namely isolates PY.01, ST.02, PY.03, S2.05, PR.01, PR.02 and ST.03 were found to produce HCN gas and inhibit F. oxysporum by more than 50%. These isolates were also able to reduce conidia production. Antagonism was also evaluated by the production of metabolites. The antifungal tests of the metabolites of 12 isolates dissolved in the supernatant showed antifungal properties and were able to inhibit the growth of F. oxysporum. The secondary metabolites of isolate PR.01 had the highest inhibition index of 73.08. Isolates ST.02, PR.02 and S2.05 were superior in inhibiting F. oxysporum and produced HCN gas and metabolites.
subject: Articles
  topic: section
subject: antagonis
  topic: keywords
subject: Fusarium oxysporum
  topic: keywords
subject: inhibition
  topic: keywords
subject: secondary metabolites
  topic: keywords
creator: Herni Susanti
creator: Fadjar Rianto
creator: Edy Syahputra
rights: Copyright (c) 2025 Jurnal Fitopatologi Indonesia
date: 2025-02-26
publication date: 2025-02-26
starting page: 234
ending page: 241
doi: 10.14692/jfi.20.5.234-241

Deteksi Odontoglossum ringspot virus pada Anggrek Asli Koleksi Kebun Raya di Indonesia
type: item
description: Native orchid is one of Indonesian natural resources which play important role as parental materials in breeding program. Virus infection is one of the limiting factors in the cultivation of orchid. The purpose of this study was to detect Odontoglossum ringspot virus (ORSV) from native orchid. Symptomatic orchids were collected from 5 botanical gardens, i.e. Bogor, Cibodas, Purwodadi, Balikpapan, and Enrekang Botanical Gardens. Detection and identification was conducted by serological method using ORSV specific antisera, followed by RT-PCR and DNA sequencing. The serological test showed that 5 samples gave positive reaction against ORSV antiserum, i.e. Phalaenopsis amboinensis (KRB2) and Phalaenopsis amabilis (KRB12) from Bogor Botanical Garden, Phalaenopsis amabilis (KRP18) and Dendrobium salacence (KRP20) from Purwodadi Botanical Garden, dan Phalaenopsis modesta J. J. Sm. (KRBp5) from Balikpapan Botanical Garden. RT-PCR of the 5 samples using specific primer of ORSV coat protein gene was successfully amplified fragment DNA with size ± 474 bp. Homology analysis of those 5 ORSV isolates showed the highest index similiarity of 99.8% with corresponding sequences from 14 other ORSV isolates. Phylogenetic analysis indicated that ORSV KRB2 and KRP18 isolates was clustered in a separate group far from ORSV isolates in other countries. This is the first report of ORSV infection on native orchids collection from 5 botanical gardens in Indonesia.
subject: Articles
  topic: section
subject: homology
  topic: keywords
subject: phylogenetic analysis
  topic: keywords
subject: RT-PCR
  topic: keywords
subject: serology
  topic: keywords
creator: Mahfut Mahfut
creator: Budi Setiadi Daryono
creator: Susamto Somowiyarjo
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-05-08
publication date: 2017-05-08
starting page: 1
ending page: 1
doi: 10.14692/jfi.13.1.1

Keragaman Morfologi, Genetika, dan Patogenisitas Colletotrichum acutatum PenyebabAntraknosa Cabai di Jawa dan Sumatera
type: item
description: Colletotrichum acutatum is known as the major causal agents of anthracnose disease of chilli pepper in Indonesia. This research was aimed to study genetic variability of C. acutatum in Java and Sumatera based on morphological and molecular characteristics and to evaluate pathogenicity of several isolates. Observation on morphological characteristics involved colour and growth of each colony as well as shape and size of conidia. Molecular identification was performed by DNA amplification using specific primer for C. acutatum, i.e. CaInt2/ITS4 followed by sequencing and nucleotide sequence analysis. Pathogenicity test for each isolate on Capsicum annuum and C. frutescens was conducted in the laboratory using detached chilli. Morphology variability was observed from 40 isolates of C. acutatum. Most of the isolates have white/beige colonies with fusiform conidia of 6.11–9.73 µm in length and 2.24­–2.73 µm in width. Pathogenicity of 3 C. acutatum isolates varies from moderate to high based on lesions size on infected chilli. Sequence analysis of 8 C. acutatum isolates indicated high homology among isolates from Java and Sumatera
subject: Articles
  topic: section
subject: colour of colony
  topic: keywords
subject: conidium
  topic: keywords
subject: DNA amplification
  topic: keywords
subject: sequence analysis
  topic: keywords
creator: Roy Ibrahim
creator: Sri Hendrastuti Hidayat
creator: widodo widodo
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-05-08
publication date: 2017-05-08
starting page: 9
ending page: 9
doi: 10.14692/jfi.13.1.9

Mekanisme Pengendalian Penyakit Busuk Batang Jeruk oleh Khamir, Kitosan, Cendawan Mikoriza Arbuskular, dan Bakteri Simbiotiknya
type: item
description: Plant diseases become an important constraint on citrus production. Stem rot disease caused by Botryodiplodia theobromae is a major disease on citrus in Indonesia. This study was aimed to evaluate the mechanism of yeast, arbuscular mychorrhiza fungi (AMF), symbiotic bacteria of AMF and chitosan in controlling stem rot disease. In vitro study was performed to evaluate the mechanism of antibiosis, hyperparasitism, production of volatile compounds, and production of chitinase enzyme. The experiment was continued by in planta assays using yeast, AMF, symbiotic bacteria of AMF, and chitosan either singly or in combination. The experiments were performed using completely randomized design with 3 replications. Disease progress were observed based on its latent period, AUDPC, infection rate, AMF association rate, and total phenol content. In vitro studies indicated that the yeasts showed hyperparasitism to B. theobromae with affinity of 26 cells per hyphae, produced volatile compounds with relative resistance level (RRL) 29.1%, and produced chitinase. Selected symbiotic bacteria from AMF showed antibiosis with RRL 42.9%, production of volatile compounds with RRL 26.7%, and has 98% homology with Bacillus subtilis from Vietnam. Application of yeast + AMF + chitosan delayed disease latent period and suppressed disease incidence. Single AMF and combination of yeast + AMF suppressed disease severity, infection rate, and AUDPC. Application of yeast + AMF showed highest association level of AMF and total phenol content. Therefore, the application of AMF and yeast + AMF is recommended in controlling citrus stem rot disease.
subject: Articles
  topic: section
subject: antibiosis
  topic: keywords
subject: Botryodiplodia theobromae
  topic: keywords
subject: chitinase
  topic: keywords
subject: hyperparasitism
  topic: keywords
creator: Hagia Sophia Khairani
creator: Meity Suradji Sinaga
creator: Kikin Hamzah Mutaqin
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-05-08
publication date: 2017-05-08
starting page: 17
ending page: 17
doi: 10.14692/jfi.13.1.17

Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat
type: item
description: Root knot nematodes (RKN) Meloidogyne spp. is the primary parasite of celery and reported to cause losses up to 70%. Identification of the Meloidogyne species on celery in Indonesia has not been reported. This study was aimed to identify the species of Meloidogyne on celery based on morphology characters. Samples of Meloidogyne-infected celery plants were taken using purposive sampling method from Ciputri Village, District of Pacet, Cianjur, West Java Province. RKN inside root tissue was detected by acid fuchsin staining method. Meloidogyne species identification was done by morphological observation of female perineal pattern. Disease symptoms found in the field include leaf yellowing, stunted, and uneven growth of celery plants. Roots of infected plants showed the formation of small size root knots, in large numbers and forming strands like a chain. Staining NPA in root tissue was successfully detected various stages of nematode development, i.e. eggs, juveniles and female nematodes. Three Meloidogyne species, namely M. incognita, M. arenaria and M. javanica were identified.
subject: Short Comunication
  topic: section
subject: acid fuchsin staining method
  topic: keywords
subject: female perineal pattern
  topic: keywords
subject: morphological identification
  topic: keywords
subject: root tissue
  topic: keywords
creator: Fitrianingrum Kurniawati
creator: Supramana Supramana
creator: Abdul Muin Adnan
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-05-08
publication date: 2017-05-08
starting page: 26
ending page: 26
doi: 10.14692/jfi.13.1.26

Penyakit Karat Bawang Daun yang Disebabkan oleh Puccinia allii Rud.
type: item
description: New disease of bunching onion (Allium fistulosum) with typical symptoms as rust disease was found in the sub-district of West Tompaso and Modoinding, District of South Minahasa, North Sulawesi in June 2016. Infected plants in the field showed yellowish brown pustules on leaf surface, followed by leaf yellowing and drying out started from the tip. Disease incidence in surveyed area reached 100% with disease severity between 30%–50%. Based on microscopic observation of the fungal morphological characteristic, i.e. uredium dan urediniospora, the causal agent was identified as Puccinia allii Rud.
subject: Disease Note
  topic: section
subject: Allium fistulosum
  topic: keywords
subject: brown pustule
  topic: keywords
subject: disease incidence
  topic: keywords
subject: urediniospora
  topic: keywords
creator: Widodo Widodo
creator: Ginting Tri Pamungkas
creator: Hendry Susetyo
creator: Antoni Setiawan
creator: Johanis Wowor
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-05-08
publication date: 2017-05-08
starting page: 31
ending page: 31
doi: 10.14692/jfi.13.1.31

Ketahanan Kultivar Pisang Lokal Kalimantan Selatan terhadap Penyakit Bercak Sigatoka (Mycosphaerella sp.)
type: item
description: Sigatoka of banana caused by Mycospherella sp. is an important disease, since it causes yield losses. Resistant variety is considered a promising approach for sigatoka disease management. This study was aimed to evaluate the resistance of local banana cultivars from South Kalimantan against sigatoka disease. The fungus was isolated from leaves of Kapas cultivar showing severe symptoms of sigatoka disease, onto potato dextrose agar medium. The isolate was then confirmed as Mycosphaerella sp. following Koch’s postulate. Level of resistance is determined based on symptoms development. Resistance of local banana cultivars can be differentiated into susceptible (‘Awak’, ‘Jaranang’, ‘Kapas’, ‘Mas’, and ‘Paikat’), medium resistant (‘Ambon’, ‘Kepok’, ‘Mas Bantan’, ‘Mauli’, and ‘Talas’), and resistant (‘Tarati’). In general, the symptom was visible 2 to 8 days after inoculation. The longer incubation period the more resistant response of banana varieties. Incubation period was negatively correlated to the disease intensity, but it is not correlated with stomatal density.
subject: Articles
  topic: section
subject: disease intensity
  topic: keywords
subject: incubation period
  topic: keywords
subject: Koch postulate
  topic: keywords
creator: Mariana Mariana
creator: Rodinah Rodinah
creator: Ismed Setya Budi
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-07-05
publication date: 2017-07-05
starting page: 51
ending page: 58
doi: 10.14692/jfi.13.2.51-58

Kepekaan Erythricium salmonicolor Penyebab Penyakit Jamur Upas pada Kemiri Sunan terhadap Suhu, Cahaya, dan Fungisida
type: item
description: A typical pink disease symptom appeared on stem of Reutealis trisperma in Bogor. The present study was aimed for confirming the causal pathogen and testing its sensitivity to temperature, light and fungicides. The fungus was identified based on its morphological characteristics. The fungal inoculum was obtained by tissue isolation of infected stem bark. For pathogenicity test, the fungus was inoculated onto sterilized pieces of woody R. trisperma branch, then fastened on the seedlings and incubated in the green house. The effect of temperatures on fungal growth was tested by planting the fungal colony onto potato dextrose agar (PDA) then incubated at 25–34 °C. The sensitivity of the fungus to light was tested by inoculating the fungus into flask with potato dextrose broth (PDB) medium on a shaker (60 rpm) exposing it to white light tube (400 luks) or in a dark condition for 15 days at 25 °C. The obtained fungal colonies were weighed. Efficacy of copper hydroxide and mancozeb against the fungal colony growth was tested by inoculating the fungus onto PDA amended with 100, 200, 300, 400, and 500 ppm, respectively, of tested fungicides. The results showed that the causal pathogen was Erythricium salmonicolor. In the pathogenicity test, cobwebs of E. salmonicolor were formed on inoculated seedlings 3 weeks after inoculation. The optimum temperature for growth of E. salmonicolor was 28 °C. There was no significant effect of light on fungal growth. Mancozeb suppressed colony of E. salmonicolor growth better than copper hydroxide.
subject: Articles
  topic: section
subject: morphological identification
  topic: keywords
subject: mancozeb
  topic: keywords
subject: copper hydroxide
  topic: keywords
subject: pathogenicity test
  topic: keywords
creator: Dini Florina
creator: Dono Wahyuno
creator: Siswanto Siswanto
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-07-05
publication date: 2017-07-05
starting page: 35
ending page: 42
doi: 10.14692/jfi.13.2.35-42

Cara Preservasi Fitoplasma dari Jaringan Kacang Tanah Bergejala Sapu untuk Deteksi DNA dengan Teknik PCR
type: item
description: Witches‘ broom of peanut caused by phytoplasma is a common disease found in Indonesia. Phytoplasma is able to be detected using polymerase chain reaction (PCR) technique. One of important factor which determine the successful of phytoplasma amplification is the DNA availability from fresh tissues. The research was aimed to evaluate some preservation methods of phytoplasma from infected plant samples. The aspects to be evaluated consisted of time (1, 2, 3, and 4 weeks), temperature (-20 °C, 4 °C, and 25 °C), and preservation medium (1X PGB buffer, 3 M NaCl, CTAB buffer, 70% ethanol, non medium, and FTA-card) for storing the fresh phytoplasma infected samples. Good preservation method will optimize the phytoplasma DNA amplification using PCR standard technique followed by nested-PCR. The results showed that preservation of samples at -20 °C, 4 °C, and 25 °C in CTAB buffer was able to maintain the tissue freshness for 4 weeks and was able to provide the DNA of either quality or quantity sufficiently for PCR detection. PCR standard using a primer pair P1/P7 showed that not all of the preserved DNA of phytoplasma were amplified positively. However, standard PCR followed by nested-PCR using primer pair fU5/rU3 was able to increase the DNA detectability. Preserved samples derived from various medium and stored for 4 weeks gave positive results. This results were in contrary with previous same samples which were detected negatively by standard PCR technique.
subject: Articles
  topic: section
subject: nested-PCR
  topic: keywords
subject: preservation
  topic: keywords
subject: peanut witches’ broom
  topic: keywords
subject: standard PCR
  topic: keywords
creator: Siska Irhamnawati Pulogu
creator: Kikin Hamzah Mutaqin
creator: Giyanto Giyanto
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-07-05
publication date: 2017-07-05
starting page: 43
ending page: 50
doi: 10.14692/jfi.13.2.43-50

Galur Isogenik Xanthomonas oryzae pv. oryzae Hasil Penyinaran Ultraviolet dan Potensinya Sebagai Penginduksi Resistensi Padi terhadap Penyakit Hawar Daun Bakteri
type: item
description: Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is an important rice disease and may cause 50% plant damage. One approach to manage this disease is using isogenic non-pathogenic strain that can induce plant resistance. This study was conducted to obtain isogenic strains of X. oryzae pv. oryzae through ultraviolet irradiation and evaluate ability of the isolates in inducing plant resistance. Bacteria X. oryzae pv. oryzae were exposed to UV for 3, 5, 10 and 15 min. A total of 99 colonies obtained were selected through pathogenicity tests, followed by confirmation using XOR-F and XOR-R2 primers. As much as 16 isogenic strain isolates were obtained with the ability to produce exopolysaccharides (EPS) ranges between 1.4–9.8 mg mL-1 compared to wild-type 6.0 mg mL-1. Five isogenic strains isolates (M313, M57, M101, M1513 and M1515) caused virulence reduction of 92.8–98.6%. Application of those five isogenic isolates prolonged incubation period of X. oryzae pv. Oryzae, i.e. 6.6–5.4 days compared to the wild-type i.e. 4.8 days after inoculation. Suppression of disease progression was also observed with AUDPC value 615.0-827.5, which was lower than those caused by wild-type and control, i.e. 863.3 and 682.5, respectively. Application of isogenic strain isolates did not affect plant growth but it increased plant productivity. Isogenic strain M101 was the best isolate with an incubation period of 5.7 days after inoculation, having the lowest AUDPC value (615.0), and yielded dry weight of 100 grains higher (2.8 g) than those of the wild-type (2.0 g).
subject: Articles
  topic: section
subject: AUDPC
  topic: keywords
subject: incubation period
  topic: keywords
subject: pathogenicity
  topic: keywords
subject: resistance inducer
  topic: keywords
creator: Fitria Yuliani
creator: Giyanto Giyanto
creator: Kikin Hamzah Mutaqin
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-07-05
publication date: 2017-07-05
starting page: 59
ending page: 66
doi: 10.14692/jfi.13.2.59-66

Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan
type: item
description: Root and basal stem rot disease caused by Xylaria sp. is a new diseases in Indonesian sugarcane plantation. The disease has been reported only occurred in sugarcane plantations in Sumatera. Limited information about the disease has slowed down disease diagnosis in the field. The research was aimed to describe symptoms, signs, and spatial distribution of the disease caused by Xylaria. A survey was conducted at a sugarcane plantation in South Sumatera (3° 26’ 16.6” S dan 104°40’ 09.8” E). Typical symptoms of the disease involved drying of all leaves and plant death, dry rot of root and basal stem, and retarded growth of ratoon cane were recorded. Stroma was found on surface of diseased stems and on soil surface of diseased plants, and spatial aggregation of diseased plants was observed in all locations. The distribution of the diseased plant aggregately and the presence of stroma on diseased plants are important characteristics of the disease diagnosis in sugarcane plantations.
subject: Short Comunication
  topic: section
subject: disease distribution
  topic: keywords
subject: disease sign
  topic: keywords
subject: ratoon
  topic: keywords
subject: Xylaria
  topic: keywords
creator: Tri Maryono
creator: Ani Widiastuti
creator: Achmadi Priyatmojo
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-07-05
publication date: 2017-07-05
starting page: 67
ending page: 71
doi: 10.14692/jfi.13.2.67-71

Kemampuan Tumbuhan Terna dalam Menekan Potensi Inokulum Rigidoporus microporus
type: item
description: White root disease caused by Rigidoporus microporus is an important disease of rubber tree and is very difficult to control. The ability of some herbaceous plant species to suppress inoculum potential and infection of R. microporus was studied in a pot trial. Nine species of herbaceous plants were examined, i.e. arrowroot (Marantha arundinacea), java curcumin (Curcuma xanthorrhiza), sansevieria (Sansevieria fasciata), Mallaca galangal (Alpinia malaccensis), greater galangal (Alpinia galanga), Indian shot (Canna indica), curcumin (Curcuma longa), wild taro (Colocasia esculenta), and water yam (Dioscorea alata). Pathogen’s inocula as mycelial colonizing rubber wood sticks were buried for 90 days in soil planted with tested plants. The results showed that formation of R. microporus rhizomorph in the soil was lower in pots planted with arrowroot, java cucurmin, sansevieria, Indian shot, and wild taro. All herbaceous plants, except sansevieria, caused suppression of inoculum viability and rhizomorph development. Further observation showed no colonization of rhizomorph nor necrosis of the root was found, except on Mallaca galangal and sansevieria.
subject: Articles
  topic: section
subject: inoculum potential
  topic: keywords
subject: rhizomorph
  topic: keywords
subject: rubber
  topic: keywords
subject: white root disease
  topic: keywords
creator: Sika Yulianti
creator: Suwandi Suwandi
creator: Nurhayati Nurhayati
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-09-06
publication date: 2017-09-06
starting page: 81
ending page: 81
doi: 10.14692/jfi.13.3.81

Identifikasi Patotipe Xanthomonas oryzae pv. oryzae dari Tanaman Padi di Sulawesi Selatan
type: item
description: Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight (BLB), one of important constraint in rice production. The pathogen is known to have many pathotypes which caused difficulties in disease control. This research was conducted to determine the distribution of X. oryzae pv. oryzae’s pathotypes in seven districts in South Sulawesi. Grouping of pathotypes was performed according to Kozaka method, i.e. based on the response of differential rice varieties. Infection of X. oryzae pv. oryzae was confirmed by PCR using specific primers XOR-R2/XOR-F. Out of 36 isolates, 29 isolates were identified as X. oryzae pv. oryzae which belong to pathotype III (6 isolates), pathotype IV (21 isolates), and pathotype XII (2 isolates). This result indicated that X. oryzae pv. oryzae pathotype IV was distributed widely in South Sulawesi.
subject: Articles
  topic: section
subject: bacterial leaf blight
  topic: keywords
subject: differential varieties
  topic: keywords
subject: PCR
  topic: keywords
creator: Asysyuura Asysyuura
creator: Abdjad Asih Nawangsih
creator: Kikin Hamzah Mutaqin
creator: Sudir Sudir
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-09-06
publication date: 2017-09-06
starting page: 73
ending page: 73
doi: 10.14692/jfi.13.3.73

Deteksi dan Identifikasi Fitoplasma yang Berasosiasi dengan Penyakit Layu Kelapa Di Pulau Derawan, Kalimantan Timur
type: item
description: Coconut is a major commodity in Derawan island as source of additional income for the farmers. Research was conducted to detect and identify phytoplasmas associated with coconut wilt disease in Derawan island. Coconut wilt disease was indicated by the typical symptoms, i.e. leaf yellowing, shorten of coconut sheaths and leaves, necrosis and collapse of old leaves, and also nut fall. The presence of phytoplasmas in phloem tissues of coconut stem was observed using fluorescence and electron microscope. Identification of phytoplasmas was carried out by nested-PCR and sequencing of the 16S rRNA gene. DNA fragment of phytoplasma with the size of 1.25 kbp was successfully amplified using primer pairs P1/P7, followed by primer pairs R16F2n/R16R2. Sequence analysis of the amplified fragments showed that phytoplasma associated with coconut wilt disease in Derawan island belongs to 16SrII (witches broom phytoplasma) and 16SrXI (ca. Phytoplasma oryzae) groups.
subject: Articles
  topic: section
subject: DNA sequencing
  topic: keywords
subject: 16SrRNA gene
  topic: keywords
subject: nested PCR
  topic: keywords
subject: phloem tissue
  topic: keywords
creator: Agus Eko Prasetyo
creator: Kikin Hamzah Mutaqin
creator: Giyanto Giyanto
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-09-06
publication date: 2017-09-06
starting page: 89
ending page: 89
doi: 10.14692/jfi.13.3.89

Deteksi dan Identifikasi Spesies Meloidogyne Penyebab Umbi Berbintil pada Kentang Asal Sulawesi Utara
type: item
description: Root-knot nematode (Meloidogyne spp.). is one of the main constraint of potato production in North Sulawesi. Little is known about Meloidogyne species infecting potatoes in North Sulawesi. Therefore, research was conducted to identify Meloidogyne spp. on potatoes in North Sulawesi and further study their relationship with related species from other countries. Infected potato tubers with pimple-like knot symptom were collected from three potato production centers, i.e. Kakenturan (South Minahasa), Purworejo (East Bolaang Mongondow) and Singsingon (…?? Bolaang Mongondow). Morphological identification was conducted based on the perineal pattern of the female; whereas molecular identification was conducted by PCR using specific primer for ITS-rDNA, followed by DNA sequencing and phylogenetic analysis. Two Meloidogyne species were identified i.e. M. javanica (samples form Kakenturan, Purworejo and Singsingon) and M. incognita (samples from Purworejo). M. javanica and M. incognita from North Sulawesi are similar to the related species from China with homology level of 97.5 % and 100 %, respectively.
subject: Articles
  topic: section
subject: homology
  topic: keywords
subject: ITS-rDNA
  topic: keywords
subject: morphology
  topic: keywords
subject: molecular identification
  topic: keywords
creator: Budi Sri Utami
creator: Supramana Supramana
creator: Giyanto Giyanto
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-09-06
publication date: 2017-09-06
starting page: 98
ending page: 98
doi: 10.14692/jfi.13.3.98

Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro
type: item
description: Chitinolytic bacteria have been reported as biocontrol agents and have the ability to produce chitinase enzymes. The objective of the research was to obtain chitinase producing bacteria that had antagonistic activity to Ganoderma boninense, a causal agent of basal stem rot on oil palm. A total of 63 isolates of chitinase producing bacteria were isolated from soil of Bukit Dua Belas National Park and oil palm plantation in Jambi Province; all was screened for their potency in inhibiting G. boninense in vitro. Three isolates designated TB04-05, SW01-11, and SW02-08 were potentially suppressed and inhibited the mycelium growth of G. boninense in vitro. Based on their specific chitinase activity, these three isolates produced the highest level of chitinase enzyme of 6.3072 U mg-1 protein, 6.0385 U mg-1 protein and 6.1279 U mg-1 protein, respectively after 24 hr incubation. Based on 16S RNA identification, strain TB04-05 had similarity with Bacillus cereus, whereas strains SW01 and SW02-08 had similarity with Bacillus thuringiensis.
subject: Short Comunication
  topic: section
subject: basal stem rot
  topic: keywords
subject: chitinase
  topic: keywords
subject: Elaeis guineensis
  topic: keywords
subject: oil palm
  topic: keywords
subject: 16S RNA
  topic: keywords
creator: Risky Hadi Wibowo
creator: Nisa Rachmania Mubarik
creator: Iman Rusmana
creator: Maggy Thenawidjaya
rights: Copyright (c) 2017 Jurnal Fitopatologi Indonesia
date: 2017-09-06
publication date: 2017-09-06
starting page: 105
ending page: 105
doi: 10.14692/jfi.13.3.105

Aplikasi Filtrat Guano terhadap Infeksi Pepper yellow leaf curl virus pada Tanaman Cabai
type: item
description: Application of Guano Filtrate on Infection of Pepper yellow leaf curl virus in Chilli Plants Pepper yellow leaf curl virus (PYLCV) is a member of Begomovirus genus, which causes yellow leaf curl disease in chili plants in Indonesia. This virus is transmitted by vector Bemisia tabaci (Hemiptera: Aleyrodidae). Research was carried out to determine the potential of guano in suppressing yellow leaf curl disease in chili pepper. Field experiment was conducted using factorial design in a randomized block design with 2 factors. The first factor was chili cultivar (‘Gelora’, ‘Bara’, and ‘Pelita 8’) and the second factor was treatment of guano filtrate (before virus inoculation, 1 week after virus inoculation, 2 weeks after virus inoculation, virus inoculation without guano, without virus inoculation nor guano). Virus inoculation was carried out using B. tabaci. In general, symptoms were developed 1 to 3 weeks after inoculation although the incubation period varied between chili cultivars. Green mosaic with leaf curling was mostly found in ‘Gelora’, whereas yellowing with leaf cupping was mostly found in ‘Bara’ and ‘Pelita 8’. Begomovirus infection on plant showing symptoms has been confirmed by polymerase chain reaction method. Application of guano did not cause suppression on disease incidence and severity. Similarly, it did not affect plant height and flowering period.
subject: Articles
  topic: section
subject: Begomovirus
  topic: keywords
subject: Bemisia tabaci
  topic: keywords
subject: disease incidence
  topic: keywords
subject: disease severity
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subject: incubation period
  topic: keywords
creator: Azmi Khoirin Nada
creator: Sri Hendrastuti Hidayat
rights: Copyright (c) 2023 Jurnal Fitopatologi Indonesia
date: 2023-03-23
publication date: 2023-03-23
starting page: 191
ending page: 199
doi: 10.14692/jfi.16.5.191-199