Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida
DOI:
https://doi.org/10.14692/jfi.10.3.87Abstract
Mottle disease of carnation (Dianthus caryophyllus) was observed in cultivation areas in Cianjur and Bandung, West Java. This study aimed to identify the virus causing leaf mottling on carnation using reverse transcription-polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. Fragment DNA of 1000 bp was successfully amplified using specific primers for coat protein of Carmovirus (BC57/BC58). Direct sequencing was proceeded using PCR products. Nucleotide sequence analysis confirmed the infection of Carnation mottle virus (CarMV) from leaves showing mottle symptom. Furthermore, phylogenetic analysis showed that CarMV isolates from Indonesia have close relationship with those from Brazil, Israel, Iran, India, Netherland, and Spain.
Downloads
Downloads
Published
Issue
Section
License
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
