First Report of Nerine latent virus Infecting Green-Tinge Spiderlily (Hymenocallis speciosa) in Indonesia: Laporan Pertama Nerine latent virus yang Menginfeksi Bunga Bakung Laba-laba (Hymenocallis speciosa) di Indonesia

Theresia Aprilia Sianipar; Didiet Rahayu Diana; Mohamed Cassim Mohamed Zakeel; Sari Nurulita

doi:10.14692/jfi.22.2.61-64

Authors

Theresia Aprilia Sianipar Department of Plant Protection, Faculty of Agriculture, IPB University
Didiet Rahayu Diana Jakarta Animal, Fish, and Plant Quarantine Office
Mohamed Cassim Mohamed Zakeel CSIRO Agriculture and Food
Sari Nurulita Department of Plant Protection, Faculty of Agriculture, IPB University

DOI:

https://doi.org/10.14692/jfi.22.2.61-64

Keywords:

carlavirus, degenerate primer, NeLV, ornamental plant, RT-PCR

Abstract

In 2024, a composite leaf sample showing irregular yellowing symptoms was collected from green-tinge spiderlily (Hymenocallis speciosa) in Bogor Regency, Indonesia. The sample (isolate SLPB2) was tested positive for carlavirus infection using a degenerate primer pair targeting the partial coat protein and nucleic acid binding protein regions, and then the amplicon was sequenced. BLAST analysis of the sequence indicated that the isolate belongs to the species Nerine latent virus (NeLV, Carlavirus latensnerinis). This solate shared 83.5%–99.8% nucleotide identity with other NeLV isolates available in GenBank, confirming its assignment to the species. The genus Carlavirus (family Betaflexiviridae) comprises several economically important spesies that infect a wide range of horticultural crops, including ornamental plants. Further disease surveillance and biological characterisation are needed to assess the distribution and impact of NeLV on H. speciosa, and to inform the development of effective management strategies. To the best of our knowledge, this is the first report of NeLV infecting green-tinge spiderlily, both in Indonesia and worldwide.

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References

Chao HY, Dietzgen RG, Thomas JE, Geering ADW. 2025. Evaluation of spider lilies (Hymenocallis spp.) as sentinel plants for orthotospoviruses. Phytopathology Research. 7(37):1–12. DOI: https://doi.org/10.1186/s42483-025-00325-5.

Chen CC, Chang CA, Chiang FL. 2016. Serological and molecular identification of Nerine latent virus infecting blood lily (Haemanthus multiflorus Martyn). Journal of Taiwan Agricultural Research. 65(2):194–206.

Cremer J, Campbell P, Steele V, Persley D, Thomas J, Harper S, Gambley C. 2021. Detection and distribution of viruses infecting garlic crops in Australia. Plants. 10:1013. DOI: https://doi.org/10.3390/plants10051013.

Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19:11–19.

Heo ES, Choi SH. 2021. Nerine latent virus diagnosed in Hippeastrum bulbs in Korea. Horticultural Science and Technology. 39(4):507–513. DOI: https://doi.org/10.7235/HORT.20210045.

[ICTV] International Committee on Taxonomy of Viruses. 2011. Family: Betaflexiviridae. In: ICTV 9th Report. Available in: https://ictv.global/report_9th/RNApos/Betaflexiviridae. Accessed on May 2nd, 2026.

Jordan R, Wingert M, Louden C, Guaragna MA. 2018. First report of nerine latent virus in ornamental Crinum in the United States. Plant Disease. 102(7):1469. DOI: https://doi.org/10.1094/PDIS-09-17-1512-PDN.

Kumar S, Stecher G, Suleski M, Sanderford M, Sharma S, Tamura K. 2024. MEGA12: molecular evolutionary genetic analysis version 12 for adaptive and green computing. Molecular Biology and Evolution. 41:1–9. DOI: https://doi.org/10.1093/molbev/msae263.

Langeveld SA, Dore JM, Memelink J, Derks AFLM, van der Vlugt CIM, Asjes CJ, Bol JF. 1991. Identification of potyviruses using the polymerase chain reaction with degenerate primers. Journal of General Virology. 72:1531–1541. DOI: https://doi.org/10.1099/0022-1317-72-7-1531.

Liu Y, Lu X, Zhi L, Zheng Y, Chen X, Xu Y, Wu F, Li Y. 2012. Calla lily chlorotic spot virus from spider lily (Hymenocallis litteralis) and tobacco (Nicotiana tabacum) in the South-west of China. Journal of Phytopathology. 160:201–205. DOI: https://doi.org/10.1111/j.1439-0434.2011.01873.x.

Maat DZ, Huttinga H, Hakkaart FA. 1978. Nerine latent virus: some properties and serological detectability in Nerine bowdenii. Netherlands Journal of Plant Pathology. 84(2):47–59. DOI: https://doi.org/10.1007/BF01976408.

Monger WA, Eden RJ. 2019. First report of nerine latent virus in Haemanthus albiflos in the UK. New Disease Reports. 39(1):3. DOI: https://doi.org/10.5197/j.2044-0588.2019.039.003.

Muhire BM, Varsani A, Martin DP. 2014. SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE. 9(9):e108277. DOI: https://doi.org/10.1371/journal.pone.0108277.

Ng JCK, Perry KL. 2004. Transmission of plant viruses by aphid vectors. Molecular Plant Pathology. 5(5):505–511. DOI: https://doi.org/10.1111/j.1364-3703.2004.00240.x.

Nurulita S, Sianipar TA, Diana DR, Hidayat SH. 2025. First report of nerine latent virus and amazon lily mosaic virus infecting amaryllis in Indonesia. New Disease Reports. 52(2):e70090. DOI: https://doi.org/10.1002/ndr2.70090.

Sastry KS, Mandal B, Hammond J, Scott SW, Briddon RW. 2019. Hymenocallis littoralis (beach spider lily). In: Encyclopedia of Plant Viruses and Viroids. New Delhi: Springer. pp 1228–1229. DOI: https://doi.org/10.1007/978-81-322-3912-3_472.

Singh AK, Hallan V, Verma N, Zaidi AA. 2005. Natural occurrence of Lily symptomless virus on spider lily. Plant Pathology. 54:255–255. DOI: https://doi.org/10.1111/j.1365-3059.2005.01122.x.

Wylie SJ, Jones MG. 2012. Complete genome sequences of seven carlavirus and potyvirus isolates from Narcissus and Hippeastrum plants in Australia, and proposals to clarify their naming. Archives of Virology. 157(8):1471–1480. DOI: https://doi.org/10.1007/s00705-012-1319-6.