Potensi Plant Growth-Promoting Bacteria Menekan Cucumber mosaic virus pada Tanaman Mentimun
The Potential of Plant Growth-Promoting Bacteria to Suppress Infection of Cucumber mosaic virus on Cucumber Plants
DOI:
https://doi.org/10.14692/jfi.21.1.38-50Keywords:
biocontrol agent, disease severity, incubation period, mechanical inoculation, seed treatmentAbstract
The Potential of Plant Growth-Promoting Bacteria to Suppress Infection of Cucumber mosaic virus on Cucumber Plants
Mosaic disease caused by Cucumber mosaic virus (CMV) is one of the main diseases of cucumber plants, and is economically important. Plant growth-promoting bacteria (PGPB) are plant root organisms currently being developed as biological agents. This research aims to obtain the best PGPB that can suppress the development of CMV in cucumber plants. This research used a randomized block design with nine treatments and five replications. The treatments consisted of several isolates of PGPB (Serratia marsescens AR1, Alcaligenes faecalis AJ14, Stenotrophomonas pavanii KJKB 5.4, Pseudomonas fluorescens LPK1–9, Bacillus cereus AJ 34, and S. maltophilia LMTSA 5.4), orthohydroxybenzoic acid (salicylic acid), positive control, and negative control. Application of PGPB was conducted through seed treatment of cucumber seeds for 15 minutes before planting. The mosaic virus was mechanically inoculated on cucumber cotyledons seven days after planting. The variables observed were the development of mosaic disease and the growth of cucumber plants. Virus detection using specific primer pair CMV-IF/CMV-IR showed that the virus that infected cucumber plants had 98.3% similarity to the CMV isolate originating from India with accession number KJ874248.1. Pseudomonas fluorescens LPK1-9 is the best bacteria for slowing the incubation period, reducing the percentage of infected leaves, and reducing the severity of mosaic disease on cucumber plants. None of tested PGPB isolates that has the potential to increase the growth of CMV-infected cucumber plants. Therefore, further studies are needed on the effectiveness of the PGPB, such as improving how PGPB is applied.
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Ajijah N, Fiodor A, Pandey AK, Rana A, Pranaw K. 2023. Plant growth-promoting bacteria (PGPB) with biofilm-forming ability: a multifaceted agent for sustainable agriculture. Diversity 15(1):112. DOI: https://doi.org/10.3390/d15010112.
Anikina I, Kamarova A, Issayeva K, Issakhanova S, Mustafayeva N, Insebayeva M, Mukhamedzhanova A, Khan SM, Ahmad Z, Lho LH, Han H, Raposo A. 2023. Plant protection from virus: a review of different approaches. Frontiers in Plant Science. 14:1163270. DOI: https://doi.org/10.3389/fpls.2023.1163270.
A’yun KQ, Hadiastono T, Martususiro M. 2013. Pengaruh penggunaan plant growth promoting rhizobacteria (PGPR) terhadap intensitas Tobacco mosaic virus (TMV) pertumbuhan, dan produksi pada tanaman cabai rawit (Capsicum frutescens L.). Jurnal Hama Penyakit Tumbuhan. 1(1):48.
Abrahamian PE, Abou-Jawdah Y. 2014. Whitefly-transmitted criniviruses of cucurbits: current status and future prospects. VirusDisease. 25(1):26–38. DOI: https://doi.org/10.1007/s13337-013-0173-9.
Agrios GN. 2005. Plant Pathology. Ed Ke-5. Burlington (MA): Elsevier Academic Press.
Agustin, Dyah A, A’yun EQ, Marsya TI, Kusuma RR. 2020. Potensi plant growth promoting bacteria (PGPB) sebagai pemacu ketahanan tanaman padi terhadap hawar malai padi. Journal of Agricultural Science. 6(2):96–105. DOI: http://dx.doi.org/10.21776/ub.jpt.2021.006.2.1.
Babadoost M. 2012. A viral disease of cucurbits. Report on Plant University of Extension. 926:1–3.
Elango R, Parthasarathi R, Megala S. 2013. Field level studies on the associa growth promoting rhizobacteria (PGPR) in Gloriosa superba L. rhizosphere. Indian Streams Research Journal. 3(10):1–6.
Farida N, Damayanti TA, Efendi D, Hidayat SH. 2022. Insidensi dan identifikasi molekuler Papaya ringspot virus pada pepaya di Jawa. Jurnal Fitopatologi Indonesia. 18(1):43–51. DOI: https://doi.org/10.14692/jfi.18.1.43-51.
Fidan H, Koç G. 2019. Occurrence, ecology and phylogeny of banana streak badnavirus (BSV) and cucumber mosaic cucumovirus (CMV) in Musa sp. production areas of the Mediterranean coastline of Turkey. Applied Ecology and Environmental Research. 17(3):5935–5951. DOI: https://doi.org/10.15666/aeer/1703_59355951.
Figueiredo MdVB, Seldin L, de Araujo FF, Mariano RdLR. 2010. Plant growth promoting rhizobacteria: fundamentals and applications. Di dalam: Maheshwari D, editor. Plant Growth and Health Promoting Bacteria. Microbiology monographs 18. Berlin (DE): Springer. hlm 21–43.
Giyanto, Purnamawati I, Damayanti TA. 2019. Potensi bakteri agens hayati untuk menekan infeksi Cucumber mosaic virus (CMV) pada melon (Cucumis melo L.). Agrovigor: Jurnal Agroekoteknologi. 12(2):87–93. DOI: https://doi.org/10.21107/agrovigor.v12i2.5834.
Hidayat SH, Nurulita S, Wiyono S. 2012. Temuan penyakit baru infeksi Papaya ringspot virus pada tanaman pepaya di Nanggroe Aceh Darussalam. Jurnal Fitopatologi Indonesia. 8(6):184–187. DOI: https://doi.org/10.14692/jfi.8.6.184.
Koenraad HMS, Remeeus PM. 2010. Detection of Squash mosaic virus, Cucumber green mottle mosaic virus, and Melon necrotic spot virus in cucurbits. Bassersdorf (CH): International Seed Testing Association.
Kumar S, Chauhan PS, Agrawal L, Raj R, Srivastava A, Gupta S, Mishra SK, Yadav S, Singh PC, Raj SK, Nautiyal CS. 2016 Paenibacillus lentimorbus inoculation enhances tobacco growth and extenuates the virulence of Cucumber mosaic virus. PLoS ONE. 11(3):e0149980. DOI: https://doi.org/10.1371/journal.pone.0149980.
Lecoq H, Desbiez C. 2012. Viruses of cucurbit crops in the Mediterranean region: an ever -changing picture. Advances in Virus Research. 84:67–126. DOI: https://doi.org/10.1016/B978-0-12-394314-9.00003-8.
Listihani, Darmayanti TA, Hidayat SH, Wiyono S. 2018. Karakterisasi molekuler Papaya ringspot virus tipe P pada tananaman mentimun di Jawa. Jurnal Fitopatologi Indonesia. 14(3):75–82. DOI: https://doi.org/10.14692/jfi.14.3.75.
Megahed AA, Lashin SM, El-Dougdoug KA, Othman BA, Ibrahim MA. 2013. Potential of biotic inducers on disease severity and variation of Cucumber mosaic cucumovirus in cucumber plants. Archives of Phytopathology and Plant Protection. 46(2):193–200. DOI: https://doi.org/10.1080/03235408.2012.736280.
Mochizuki T, Ohki ST. 2012. Cucumber mosaic virus: viral genes as virulence determinants. Molecular Plant Pathology 13(3):217–225. DOI: https://doi.org/10.1111/j.1364-3703.2011.00749.x.
Mochizuki T, Yamazaki R, Wada T, Ohki ST. 2014. Coat protein mutations in an attenuated Cucumber mosaic virus Encoding mutant 2b protein that lacks RNA silencing suppressor activity induces chlorosis with photosynthesis gene repression and chloroplast abnormalities in infected tobacco plants. Virology. 456–457:292–299. DOI: https://doi.org/10.1016/j.virol.2014.04.010.
Nordam D. 1973. Identification of Plant Viruses, Methods and Experiment. Wageningen (DE): Centre for Agricultural Publishing and Documentation.
Ntui VO, Kong K, Azadi P, Khan RS, Chin DP, Igawa T, Mii M, Nakamura I. 2014. RNAi-mediated resistance to Cucumber mosaic virus (CMV) in genetically engineered tomato. American Journal of Plant Sciences 5(5):554572. DOI: https://www.cabidigitallibrary.org/doi/full/10.5555/20143125537
Perotto MC, Pozzi EA, Celli MG, Luciani CE, Mitidieri MS, Conci VC. 2018. Identification and characterization of a new potyvirus infecting cucurbits. Archives of Virology. 163:719–724. DOI: https://doi.org/10.1007/s00705-017-3660-2.
Priwiratama H, Hidayat SH. 2012. Pengaruh empat galur bakteri pemacu pertumbuhan tanaman dan waktu inokulasi virus terhadap keparahan penyakit daun keriting kuning cabai. Jurnal Fitopatologi Indonesia. 8(1):1–8. DOI: https://doi.org/10.14692/jfi.8.1.1.
Rahma H, Zainal A, Sinaga MS, Surahman M, Giyanto. 2014. Potensi bakteri endofit dalam menekan penyakit layu Stewart (Pantoea stewartia subsp. stewartii) pada Tanaman Jagung. Jurnal Hama dan Penyakit Tumbuhan Tropika. 14(2):121–127. DOI: https://doi.org/10.23960/j.hptt.214121-127.
Rahma H, Nurbailis, Kristina N. 2019. Characterization and potential of plant growth promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae. Jurnal Biodiversitas. 20(12):3655–3661. DOI: https://doi.org/10.13057/biodiv/d201226.
Reddy PP. 2010. Bacterial Viral Disease and Their Management in Horticultural Crops. Banglore (IN): Scientific Publishers.
Ridho MA, Fadli, Martinius, Liswarni Y, Najmi L, Trisno J. 2023. Kejadian pertama infeksi Zucchini yellow mosaic virus pada tanaman mentimun di Padang, Sumatera Barat. Jurnal Fitopatologi Indoenesia. 19(5):183–187. DOI: https://doi.org/10.14692/jfi.19.5.183-187.
Sharma S, Gümüş, M. 2023. Biological and molecular detection of Cucumber mosaic virus (CMV) isolates obtained from Izmir. Akademik Ziraat Dergisi. 12(2):199–210. DOI: https://doi.org/10.29278/azd.1318370.
Shehata WF, Iqbal Z, Abdelbaset TE, Saker KI, El Shorbagy AE, Soliman AM, Sattar MN, El-Ganainy SM. 2023. Identification of a Cucumber mosaic virus from Cucurbita pepo on new reclamation land in Egypt and the changes induced in pumpkin plants. Sustainability. 15(12):9751. DOI: https://doi.org/10.3390/su15129751.
Sivan A, Chet I. 1986. Biological control of Fusarium spp. in cotton wheat and muskmelon by Trichoderma harzianum. Journal of Phytopathology. 116(1):39–47. DOI: https://doi.org/10.1111/j.1439-0434.1986.tb00892.x.
Sutrawati M, Sipriyadi S, Sihotang YKS, Hutasoit CM, Parwito P, Aulia E. 2021. Tomato Leaf Curl New Delhi Virus Associated with Yellow Mosaic Disease of Cucumber (Cucumis sativus) in Bengkulu, Indonesia. Di dalam: Proceedings of the 7th International Conference on Biological Science. Series: Advances in Biological Sciences Research Vol 22; 2021 Okt 14-15; Yogyakarta (ID): Universitas Gadjah Mada. hlm 1-4.
Taufik M, Astuti AP, Hidayat SH. 2005. Survei infeksi Cucumber mosaic virus dan Chilli veinal mottle virus pada tanaman cabai dan seleksi ketahanan beberapa kultivar cabai. Jurnal Agrikultura 16:146–152.
Trisno J, Habazar T, Jamsari, Hidayat SH. 2013. Penapisan kemampuan isolat rizobakteri indigenus dalam meningkatkan ketahanan tanaman cabai terhadap penyakit virus daun kuning keriting. Di dalam: Prosiding Seminar Nasional dan Rapat Tahunan Dekan Bidang Ilmu Pertanian BKS Wilayah Barat; 2013 Mar 14-20; Pontianak (ID). hlm 889–902.
Yanti Y, Resti Z. 2010. Induksi ketahanan tanaman bawang merah dengan bakteri rhizoplan indigenos terhadap penyakit hawar daun bakteri (Xanthomonas axonopodis pv allii). Di dalam: Soesanto L, Mugiastuti E, Rahayuniati RF, Manan A, editor. Prosiding Seminar Nasional Pengelolaan OPT Ramah Lingkungan; 2010 Nov 10-11; Purwokerto (ID). hlm 235–241.
Yanti Y, Habazar T, Resti Z, Suhalita D. 2013. Penapisan isolat rizobakteri dari perakaran tanaman kedelai yang sehat untuk pengendalian penyakit pustul bakteri (Xanthomonas axonopodis pv. glycines). Jurnal Hama dan Penyakit Tumbuhan Tropika. 13(1):24–34. DOI: https://doi.org/10.23960/j.hptt.11324-34.
Wahyuni WS, Addy HS, Arman B, Setyowati TC. 2006. Sinergisme Lumbricus rubellus dengan Pseudomonas putida Pf-20 dalam menginduksi ketahanan mentimun terhadap Cucumber mosaic virus. Hayati. 13(3):95–100. DOI: https://doi.org/10.1016/S1978-3019(16)30300-X.
Wan NF, Fu L, Dainese M, Kiær LP, Hu YQ, Xin F, Goulson D, Woodcock BA, Vanbergen AJ, Spurgeon DJ, Shen S, Scherber C. 2025. Pesticides have negative effects on non-target organisms. Nature Communications. 16:1360. DOI: https://doi.org/10.1038/s41467-025-56732-x.
Zitter TA, Murphy JF. 2009. Cucumber Mosaic Virus. The Plant Health Instructor. DOI: https://doi.org/10.1094/PHI-I-2009-0518-01.
Zhou Y, Bai Y. 2025. The functional mechanisms, screening methods, and agricultural application potential of plant growth-promoting bacteria. Geographical Research Bulletin. 4(20):158–174. DOI: https://doi.org/10.50908/grb.4.0_158.
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