Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa

Authors

  • Umi Kulsum Balai Besar Peramalan Organisme Pengganggu Tumbuhan, Karawang
  • Sedyo Hartono Universitas Gadjah Mada
  • Sri Sulandari Universitas Gadjah Mada
  • Susamto Sumowiyarjo Universitas Gadjah Mada

DOI:

https://doi.org/10.14692/jfi.12.6.224

Keywords:

amplification, coat protein, mosaic, variety Anjasmoro

Abstract

Soybean (Glycine max) is an important  food crops in Indonesia, but the production is considered low. Infection of Cowpea mild mottle virus (CPMMV) may affect to the productivity of the crops.  The virus cause a variety of symptoms on soybean such as mosaic, chlorosis, necrosis, distortion, and leaf dwarf. The aims of this study were to detect and identify CPMMV by molecular technique from some varieties of soybeans, i.e.  ‘Anjasmoro’, ‘Argomulyo’, ‘Grobogan’, and two local Edamame varieties. Symptomatic samples was obtained randomly from main soybean growing areas in Java.  DNA amplification using specific primer for coat protein of CPMMV was only successfully gained from leaf samples of var. Argomulyo from Malang which showing mosaic on the leaf surface, vein clearing, and chlorosis. Further amino acid and nucleotide sequence analysis showed that this CPMMV isolate has highest homology (99%) to CPMMV isolate of French bean from China.

Downloads

Download data is not yet available.

Downloads

Published

2017-03-08

Issue

Vol. 12 No. 6 (2016)

Section

Short Comunication

License

Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:

1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.

3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

How to Cite

Kulsum, U., Hartono, S., Sulandari, S., & Sumowiyarjo, S. (2017). Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa. Jurnal Fitopatologi Indonesia, 12(6), 224. https://doi.org/10.14692/jfi.12.6.224