Forty healthy and non-pregnant Fries Holland dairy cows which had received oestrus synchronization were divided randomly into four experimental groups. Artificial inseminations (AI) were implemented 8-10 hours post estrous using frozen semen from the same breed. The first three groups were injected with 100, 150 and 200 mg medroxy progesterone acetate (MPA) i.m., respectively, three days post AI, while the other group was injected with placebo as control group. Madura cattle embryos were transfered contralaterally seven days post AI. The percentage of pregnancy in groups which had received 100 and 150 mg MPA was 37.5%r, and in group received 200 mg MPA and control was 55.6%, and 60%, (P>0.05), respectively. Calving percentages of the 100, 150, 200 mg MPA and the control groups were 25,37.5,55.6 and 60% respectively (P>0.05). All birth gave single calve.
Sixteen isolates of group C streptococci taken during outbreak in pigs and monkeys in Bali were examined for their haemaglutination activities using 2% erythrocyte suspension from pigs. Five isolates (31,25%) showed very strong (++) result with geometric titer mean of 22.8, other isolates (31,25%) gave strong (+) results with geometric titer mean of 22 and the remain six isolates (37,5%) showed weak or negative haemagglutination activities. Nonencapsulated bacteria yielded haemagglutination activities stronger than encapsulated. Treatments with heating up to 90 °C, HCl extraction and opsonization reduced haemagglutination titers.
