The research was aimed to evaluate the inclusion effect of dahlia tubers inulin (DTI) combined with Lactobacillus sp. in dietary protein step down on villi development and growth of KUB chicken. The experiment was arranged in a completely randomized design using 160 birds of one-day-old KUB chicks (body weight was 26.32±1.19 g) divided into 5 treatments with 4 replications. The treatment applied were: (1) P180S: ration with 18% protein with the addition of 1.2% DTI and 1.2 mL (108 cfu/mL) Lactobacillus sp. provided since one-day-old, (2) P15: ration with 15% protein without DTI and Lactobacillus addition and was given from 8-day old, (3) P18: ration with 18% protein without DTI and Lactobacillus addition and was given from 8-day old, (4) P15S: ration with 15% protein with the addition of 1.2% DTI and 1.2 mL Lactobacillus sp. offered from 8-day old, and (5) P18S: ration with 18% protein with the addition of 1.2% DTI and 1.2 mL Lactobacillus sp. offered from 8-day old. The experimental birds allocated in the treatments 2 to 5 were fed ration with 21% protein from hatching until 7-day old. Variables observed were the jejunal pH, total Coliform and lactic acid bacteria (LAB), villi height, protein digestibility, final body weight, and carcass percentage. Data were subjected to analysis of variance (ANOVA), and followed by orthogonal contrast test. Orthogonal contrast test showed that feeding 18% dietary protein fortified with a combination of 1.2% inulin dahlia tuber and 1.2 mL Lactobacillus sp. given since one-day-old significantly (P<0.05) affected the height of jejunal villi, protein digestibility, carcass percentage, final body weight, jejunal pH, and Coliform number. In conclusion, feeding 18% dietary protein fortified with a combination of 1.2% of inulin dahlia tuber and 1.2 mL of Lactobacillus sp. since one-day-old increases the height of the jejunum villi and growth of KUB chicken.
Abdurrahman, Z.H., Y. B. Pramono, & N. Suthama. 2016b. Meat characteristic of crossbred local chicken fed inulin of dahlia tuber and Lactobacillus sp. Med. Pet. 39: 112-118. https://doi.org/10.5398/medpet.2016.39.2.112
Arifin, M. & V. J. Pramono. 2014. Pengaruh pemberian sinbiotik sebagai alternatif pengganti antibiotic growth promoter terhadap pertumbuhan dan ukuran villi usus ayam broiler. J. Sains Vet. 32: 205-217.
Awad, W.A., K. Ghareeb, S. Abdel-Raheem, & J. Bohm. 2009. Effect of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult. Sci. 88: 49-55. https://doi.org/10.3382/ps.2008-00244
Balton, W. 1967. MAFF Bulletin. No. 174. Poultry Nutrition. HMSO, London.
Beski. S.S.M. & S.Y.T. Al-Sardary. 2015. Effect of dietary suplementation of probiotic and synbiotic on broiler chickens Hematology and intestinal integrity. Poult. Sci. 14: 31-36. https://doi.org/10.3923/ijps.2015.31.36
Carvalho, N. & S. Hansen. 2005. Prospect for probiotics in broiler. Feed Int. 26:9-12.
Cheng, Y., Y. Chen, X. Li, W. Yang, C. Wen, Y. Kang, A. Wang, & Y. Zhou. 2017. Effect of synbiotic suplementation on growth performance, carcass characteristic, meat quality and muscular antioxidant capacity and mineral content in broilers. J. Sci. Food Agric. 97: 3699-3705. https://doi.org/10.1002/jsfa.8230
Erdogan, Z., S. Erdogan, O. Aslantas, & S. Celik. 2010. Effect of dietary supplementation of synbiotics and phytobiotics on performance, caecal Coliform population and some oxidant/antioxidant parameters of broliers. J. Anim. Physiol. Anim. Nutr. (Berl) 94: e40-8. https://doi.org/10.1111/j.1439-0396.2009.00973.x
Fajrih, N., N. Suthama, & V. D. Yunianto. 2014. Body resistance and productive performances of crossbred local chicken fed inulin of dahlia tubers. Med. Pet. 37: 108-114. https://doi.org/10.5398/medpet.2014.37.2.108
Faradila, S., N. Suthama, & B. Sukamto. 2016. Kombinasi inulin umbi dahlia-Lactobacillus sp. yang mengoptimalkan perkembangan mikroflora usus dan pertumbuhan persilangan ayam Pelung-Leghorn. J. Vet. 17: 168-175.
Fardiaz, S. 1992. Analisis Mikrobiologi Pangan. Raja Grafindo Persada, Jakarta.
Hamid, I.S., B. P. S. Rahardjo, & M. Gabriela. 2014. Potensi pemberian sinbiotik pada umur yang berbeda pada gambaran histologi ileum ayam pedaging betina. J. Vet. Medika 7: 114-119
Harimurti, S. & E. S. Rahayu. 2009. Morfologi usus ayam broiler yang disuplementasi dengan probiotik strain tunggal dan campuran. Agritech 29: 179-183.
Huiling, Y., G. Yupeng, & Z. Liyong. 2011. Effect of dietary synbiotics on intestinal microbial ecology in broilers chickens. Scientia Agricultura Sinica. 44: 4882-4891.
Indreswari, R., H.I. Wahyuni, N. Suthama, & P.A. Ristiana. 2009. Calcium utilization for egg shell formation as affected by feeding ration in the morning and afternoon in layer chicken (In bahasa Indonesia). J. Indonesian Trop. Anim. Agric. 34: 134-138.
Kabir, S. M. L. 2009. The role of probiotics in the poultry industry. Int. J. Mol. Sci.10: 3531-3546. https://doi.org/10.3390/ijms10083531
Krismiyanto, L., N. Suthama, & H.I. Wahyuni. 2014. Feeding effect of inulin derived from dahlia variabilis tuber on intestinal microbes in starter period of crossbreed native chicken. J. Indonesian Tropis. Anim. Agric. 39: 217-223.
McDonald, P., R.A. Edward, J.F.G. Greenhalgh, & C.A. Morgan. 2002. Animal Nutrition. 6th ed. Gosport.
Min, Y.N., H.L. Yang, Y.X. Xu, & Y.P. Gao. 2016. Effect of dietary supplementation of synbiotics on growth performance, intestinal morphology, SlgA content and antioxidant capacities of broliers. J. Anim. Physiol. Anim. Nutr. (Berl) 100: 1073-1080. https://doi.org/10.1111/jpn.12479
Nabizadeh, A. 2012. The effect of Inulin on broiler chicken intestinal microflora, gut morphologi and performance. J. Anim. Sci. 21: 725.
Ohashi, Y. & K. Ushida. 2009. Health-beneficial effects of probiotics: Its mode of action. J. Anim. Sci. 80: 361-371. https://doi.org/10.1111/j.1740-0929.2009.00645.x
Ravangard., M. Houshmand, M. Khajavi, & R. Naghiha. 2017. Performance and cecal bacteria counts of broilers fed low protein diets with and without a combination of probiotic and prebiotic. Braz. J. Poult. Sci. 19: 75-82.
Rehman, H., C. Rosenkranz, J. Bohm, & J. Zentek. 2007. Dietary inulin affects the morphology but not the sodium-dependent glucose and glutamine transport in the jejunum of broilers. Poult. Sci. 86: 118-122. https://doi.org/10.1093/ps/86.1.118
Roberfroid, M.B. 2007. Inulin-type fructans: Functional food ingredients. J. Nutr. 137: 2493S-2502S. https://doi.org/10.1093/jn/137.11.2493S
Sartika, T. 2016. Panen Ayam Kampung 70 hari. Penebar Swadaya, Jakarta.
Suthama, N. Y.B. Pramono, & B. Sukamto. 2018. Improvement of broiler meat quality due to dietary inclusion of soybean oligosaccharide derived from soybean meal extract. IOP Conf. Ser.: Earth Environ. Sci. 102 012009. https://doi.org/10.1088/1755-1315/102/1/012009
Wahyuni, H.I, N. Suthama, I. Mangisah, & L. Krismiyanto. 2018. Improving protein mass and cumulative body weight gain of local chicken fed ration fortified with a combination of Lactobacillus sp. and dahlia inulin. IOP Conf. Ser.: Earth Environ. Sci. 102 012072.
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