@article{Widianingrum_Khasanah_Addy_2022, title={Presence of Antibiotic-Resistant in Staphylococcal Subclinical Mastitis in Several Regencies of East Java, Indonesia}, volume={45}, url={https://journal.ipb.ac.id/index.php/tasj/article/view/34875}, DOI={10.5398/tasj.2022.45.1.91}, abstractNote={<p><em>Staphylococcal</em> mastitis has been reported as a serious dairy disease in various regions around the world. The occurrence of resistant strains in <em>Staphylococcus</em> species to antibiotics has triggered alternative treatment substituting antibiotic usage on the global scene. This study aimed to investigate the presence of antibiotic-resistant genes in <em>Staphylococcal</em> subclinical-mastitis cases present in several regencies of East Java Province, Indonesia. A total of 592 quarter milk samples were collected from 62 farms in the region with high dairy cattle populations in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical-mastitis samples were screened using the California mastitis test (CMT). Positive CMT samples were grown on the selective <em>Staphylococcus</em> media and tested for their biochemical properties. The polymerase chain reaction was performed to detect the presence of antibiotic-resistant genes in all isolates (<em>Staphylococcus</em> sp) using a specific pair-primer for <em>mecA, blaZ, tetK</em>, and <em>tetM</em> genes. The result showed that about 67% of milk samples were subclinical mastitis in several regencies of East Java. About 17.12% of subclinical mastitis was caused by <em>Staphylococcus</em> species (<em>Staphylococcus aureus</em>, <em>Staphylococcus epidermidis</em>, and other non-<em>aureus Staphylococci</em> (NAS)). The most prevalent region of <em>Staphylococcal</em> subclinical-mastitis was recorded in Jember. However, only NAS species obtained from Mojokerto, Malang, Probolinggo, and Banyuwangi were detected to have a <em>blaZ</em> gene responsible for penicillin resistance. In conclusion, the appearance of the antibiotic-resistant gene in NAS species found in several regencies of East Java can be used as important information to evaluate <em>Staphylococcal</em> subclinical-mastitis treatment.</p&gt;}, number={1}, journal={Tropical Animal Science Journal}, author={WidianingrumD. C. and KhasanahH. and AddyH. S.}, year={2022}, month={Feb.}, pages={91-97} }