Polymorphism Identification of Pit1 Gene in Indonesian Buffaloes (Bubalus bubalis) and Holstein-Friesian Cows

  • R Misrianti
  • C Sumantri
  • A Farajallah
DOI: https://doi.org/10.5398/medpet.2010.33.3.131
Keywords: buffalo, Pit1 gene, PCR RFLP

Abstract

Pit-1 gene has been identified as the pituitary specific transcription factor that regulates the expression of the growth hormone (GH) and prolactin (PRL) genes in the anterior pituitary. The use of polymorphic markers in breeding programmes could make selection more accurate and efficient. A total  of 320 Indonesian buffaloes from four populations and 45 FH cows from nine populations were genotyped for polymorphism of Pit1|Hinf1 gene by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods. The gene polymorphism was detected only in FH cattle. The frequency of AA, AB, and BB genotype was 2%, 44%, and 53% respectively. The frequency of A allel was 25% and B allel was 75%, but no polymorphism was detected in 320 Indonesia buffaloes.

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Author Biographies

R Misrianti

Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
Jln. Agatis, Kampus IPB Darmaga Bogor 16680, Indonesia

C Sumantri

Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University

Jln. Agatis, Kampus IPB Darmaga Bogor 16680, Indonesia

A Farajallah

Department of Biology, Faculty of Mathematic and Natural Science, Bogor Agricultural University
Darmaga Campus, Bogor 16680

Published
2011-02-24
Issue
Vol. 33 No. 3 (2010): Media Peternakan
Section
Articles
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