Genetic Analysis Using Partial Sequencing of Melanocortin 4 Receptor (MC4R) Gene in Bligon Goat

  • Latifah Latifah Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada
  • D. A. Priyadi Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada
  • D. Maharani Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada
  • Kustantinah Kustantinah Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada
  • T. Hartatik Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada
Keywords: MC4R, Bligon goat, genetic analysis, sequencing, SNP

Abstract

Melanocortin 4 Receptor gene is involved in sympathetic nerve activity, adrenal and thyroid functions, and media for leptin in regulating energy balance and homeostasis. The aim of this research was to perform genetic analysis of MC4R gene sequences from Bligon goats. Fourty blood samples of Bligon does were used for DNA extraction. The primers were designed after alignment of 12 DNA sequences of MC4R gene from goat, sheep, and cattle. The primers were constructed on the Capra hircus MC4R gene sequence from GenBank (accession No. NM_001285591). Two DNA polymorphisms of MC4R were revealed in exon region (g.998 A/G and g.1079 C/T). The SNP g.998 A/G was a non-synonymous polymorphism i.e., changing of amino acid from methionine (Met) to isoleucine (Ile). The SNP g.1079 C/T was a synonymous polymorphism. Restriction enzyme mapping on Bligon goat MC4R gene revealed three restriction enzymes (RsaI (GT’AC), Acc651 (G’GTAC_C), and KpnI (G_GTAC’C), which can recognize the SNP at g.1079 C/T. The restriction enzymes may be used for genotyping of the gene target using PCR-RFLP method in the future research.

Downloads

Download data is not yet available.

References

Cai, X., T. D. Mipam, F. F. Zhao, & L. Sun. 2015. SNPs detected in the yak MC4R gene and their association with growth traits. J. Animal. 9: 1097-1103. https://doi.org/10.1017/S1751731115000397

Deng, T. X., C. Y. Pang, M. Q. Liu, C. Zhang, & X. W. Liang. 2016. Synonymous single nucleotide polymorphisms in the MC4R gene that are significantly associated with milk production traits in water buffaloes. J. Gen. Mol. Res. 15:1-8. https://doi.org/10.4238/gmr.15028153

Du, X. H., C. Chen, Z. R. Yuan, L. M. Zhang, X. J. Chen, Y. H. Wang, X. Gao, L. P. Zhang, H. J. Gao, J. Y. Li, & S. Z Xu. 2013. Genetic polymorphisms of MC4R and IGF2 gene association with feed conversion efficiency traits in beef cattle. J. Vet., 33: 418-422.

Dubern, B. 2015 MC4R and MC3R Mutations. In M.L. Frelut (Ed.), The ECOG’s eBook on Child and Adolescent Obesity. Available at ebook.ecog-obesity.eu. [9 February 2016].

Fontanesi, L., L. Buttazzoni, G. Galimberti, D.G. Calo, E. Scotti, & V. Russo. 2013. Association between Melanocortin 4 Receptor (MC4R) gene haplotypes and carcass and production traits in Italian Large White pigs evaluated with a selective genotyping approach. J. Livestock. Sci. 157:48-56. https://doi.org/10.1016/j.livsci.2013.07.006

Galve A., C. Burgos, L. Silio, L. Varona, C. Rodríguez, C. Ovilo, & P. Lopez-Buesa. 2012. The effects of Leptin Receptor (LEPR) and Melanocortin-4 Receptor (MC4R) polymorphisms on fat content, fat distribution and fat composition in a Duroc×Landrace/Large White cross. J. Livestock. Sci. 145: 145–152. https://doi.org/10.1016/j.livsci.2012.01.010

Gregorio, P. D., A. D. Trana, P. Celi, S. Claps, & A. Rando. 2014. Comparison of goat, sheep, cattle and water buffalo leptin (LEP) genes and effect of the intron 1 microsatellite polymorphism in goat. Anim. Prod. Sci. 54: 1258–1262. https://doi.org/10.1071/an14101

Hartatik, T. 2014. Analisis Genetik Ternak Lokal. Gadjah Mada University Press. Yogyakarta: 75-88.

Hartatik, T. 2016. Pendekatan Praktis Deteksi Polimorfisme DNA Sapi Aceh. Gadjah Mada University Press. Yogyakarta:118-134.

Jun, Z., Q. Yong, Z. Sheng, C. Hua, W. H. Li, L.Y. Xia, & C. S. Xian. 2013. Single nucleotide polymorphisms of gene fragment of melanocortin receptor-4 C-terminal and its association with body weight in Boer goats. Jiangsu J. Agr. Sci. 6. (Abstract).

Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120. https://doi.org/10.1007/BF01731581

Liu, H., W. Tian, L. Zang, H. Wang, & H. Cui. 2010. Mutations of MC4R gene and its association with economic traits in Qinchuan cattle. J. Mol. Biol. Rep. 37:535–540. https://doi.org/10.1007/s11033-009-9706-0

Mignone F., C. Gissi, & S. Liuni. 2002. Untranslated regions of mRNAs (Review). Gen. Bio. 3:1-10.

NCBI. 2015. MC4R melanocortin 4 receptor Capra hircus (goat). Availabel at http://www.ncbi.nlm.nih.gov/gene/?term=mc4r%2C+goat. [13April 2016].

Pakpahan, S., W. T. Artama, R. Widayanti, & I. G. Suparta. 2015. Genetic variations and the origin of native Indonesian goat breeds based on mtDNA D-Loop sequences. Asian J. Anim. Sci. 9 : 341-350. https://doi.org/10.3923/ajas.2015.341.350

Pakpahan, S., W. T. Artama, & I. G. Suparta. 2016. Genetic characteristics and relationship in different goat populations of Indonesia based on Cytochrome B gene sequences. Asian J. Anim. Sci. 10: 29-38. https://doi.org/10.3923/ajas.2016.29.38

Piorkowska, K., M. Tyra, M. Rogoz, K. R. Molik, M. Oczkowicz, & M. Rozycki. 2010. Association of the melanocortin-4 receptor (MC4R) with feed intake, growth, fatness and carcass composition in pigs raised in Poland. J. Meat Sci. 85: 297–301. https://doi.org/10.1016/j.meatsci.2010.01.017

Schroyen, M., S. Janssens, A. Stinckens, M. Brebels, F. Bertolini, C. Lamberigts, K. Bekaert, L. Vanhaecke, M. Aluwe, F.A.M. Tuyttens, S. Millet, & N. Buys. 2015. The MC4R c.893G>A mutation: A marker for growth and leanness associated with boar taint odour in Belgian pig breeds. J. Meat Sci. 101: 1-4. https://doi.org/10.1016/j.meatsci.2014.08.009

Seong, J., S. S. Dong, D. P. Kyung, K. L. Hak, & S. K. Hong. 2011. Identification and analysis of MC4R polymorphisms and their association with economic traits of Korean cattle (Hanwoo). J. Mol. Biol. Rep. 39: 3597-3601. https://doi.org/10.1007/s11033-011-1133-3

Song, X. M., J. F. Jiang, G. Z. Zhang, F. X. Shiand, & Y. Q. Jiang. 2012. DNA polymorphisms of the Hu sheep melanocortin-4 receptor gene associated with birth weight and 45-day weaning weight. J. Gen. Mol. Res. 11: 4432-4441. https://doi.org/10.4238/2012.September.27.3

Wang, Y., C. Wang, J. Zhang, C. Meng, X. Zhang, Z. Wang, Y. Fang, D. Mao, & S. Cao. 2015. Three novel MC4R SNPs associated with growth traits in Hu sheep and East Friesien x Hu crossbreed sheep. Small Rum. Res. 125: 26-33. https://doi.org/10.1016/j.smallrumres.2015.02.007

Williams, R. J. 2003. Review: Restriction endonucleases. J. Mol. Biotec. 23: 225-243. https://doi.org/10.1385/MB:23:3:225

Zhang, C., Y. H. Wang, H. C. X. Y. Lan, C. Z. Lei, & X. T. Fang. 2009. Association between variants in the 5’-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle. J. Mol. Biol. Rep. 36:1839–1843. https://doi.org/10.1007/s11033-008-9388-z

Zuo, B., G. Liu, Y. Peng, H. Qian, J. Liu, X. Jiang, & A. Mara. 2014. Melanocortin-4 receptor (MC4R) polymorphisms are associated with growth and meat quality traits in sheep. J. Mol. Biol. Rep. 41: 6967-6974. https://doi.org/10.1007/s11033-014-3583-x

Published
2017-08-22