Improving Libido and Sperm Quality of Bali Bulls by Supplementation of Moringa oleifera Leaves
Moringa oleifera (Moringa) leaves contain high levels of nutrition that can be utilized as a feed supplement to increase libido and semen quality. This study was aimed to evaluate the effect of Moringa leaves supplementation on libido and sperm quality of Bali bulls. This study used two-sample t-test on four Bali bulls were kept under individual pens for two periods of eight weeks. During the first period (control), the experimental Bali bulls were fed concentrates (1% of body weight) and rice straw ad libitum. During the second period, the experimental Bali bulls were fed similar to the first period with an additional of Moringa leaves 15% of the weight of the concentrate (treatments). Libido and sperm quality were measured twice a week. Plasma samples were taken three times a day (06:00, 14:00, and 22:00h) on the last day of each period. Plasma testosterone concentrations were analyzed by using ELISA techniques. Variables measured were compared using paired student's t-test. Supplementation of Moringa leaves increased testosterone levels (P<0.05) (4.57 vs 4.79, 0.45 vs 4.78, and 2.35 vs 5.63 ng/mL, respectively during 06.00; 14.00; and 22.00h), increased libido (P<0.05) (7.20±1.49 vs 3.49±0.40 min), and increased both the total motility (P<0.05) (63.99±3.37% vs 84.96±3.09%) and the progressive motility (P<0.05) (52.77±1.76% vs 67.03±3.74%) of sperm. Supplementation of Moringa leaves also increased (P<0.05) the velocity and the amplitude and decreased (P<0.05) the linearity of sperm. It can be concluded that the supplementation of Moringa leaves could increase plasma testosterone concentrations, libido, and sperm motility of Bali bulls.
Abu, A. H., T. Ahemen & P. Ikpechukwu. 2013. The testicular morphometry and sperm quality of rabbit bucks fed graded levels of Moringa oleifera leaf meal (MOLM). Agrosearch 13:49-56. https://doi.org/10.4314/agrosh.v13i1.5
Begum H., A. Moniruddin, & K. Nahar. 2009. Environmental and nutritional aspect in male infertility. J. Medicine 10:16-19. https://doi.org/10.3329/jom.v10i1.1997
Bhakat, M., T. K. Mohanty, V. S. Raina, A. K. Gupta, H. M. Khan, R. K. Mahapatra, & M. Sarkar. 2011. Effect of age and season on semen quality parameters in Sahiwal bulls. Trop. Anim. Health. Prod. 43:1161-1168. https://doi.org/10.1007/s11250-011-9817-1
Chauhan, N. S., V. Sharma, V. K. Dixit, & M. Thakur. 2014. A review on plants used for improvement of sexual performance and virility. BioMed. Res. Int. 2014 Article ID 868062, 19 pages. https://doi.org/10.1155/2014/868062
Cheah, Y. & W. Yang. 2011. Functions of essential nutrition for high-quality spermatogenesis. Adv. Biosci. Biotechnol. 2:182-197. https://doi.org/10.4236/abb.2011.24029
Dafaalla, M.M., A. W. Hassan, O. F. Idris, S. Abdoun, G. A. Modawe & A. S. Kabbashi. 2016. Effect of ethanol extract of Moringa oleifera leaves on fertility hormone and sperm quality of Male albino rats. World J. Pharm. Res. 5:1-11.
Dhakar, R. C., S. D. Maurya, B. K. Pooniya, N. Bairwa, M. Gupta, & Sanwarma. 2011. Moringa: The herbal gold to combat malnutrition. Chron Young Sci. 2: 119-125. https://doi.org/10.4103/2229-5186.90887
DRG International, Inc., USA. 2009. DRG® Testosterone ELISA (EIA-1559). www.drg-international.com/ifu/eia-1559.pdf (10 June 2016).
Eghbali, M., S. M. Alavi-Shoushtari, S. Asri-Rezaei, & M. H. K. Ansari. 2010. Calcium, magnesium and Total Antioxidant Capacity (TAC) in seminal plasma of Water Buffalo (Bubalus bubalis) bulls and their relationships with semen characteristics. Vet. Res. Forum 1:12-20.
Ferreira, P. M. P., D. F. Farias, J. T. A. Oliveira & A. F. U. Carvalho. 2008. Moringa oleifera: Bioactive compounds and nutritional potential. Rev. Nutr. 21 Campinas:431-437. http://dx.doi.org/10.1590/S1415-52732008000400007.
Fitri, A., T. Toharmat, D. A. Astuti, & H. Tamura. 2015. The potential use of secondary metabolites in Moringa oleifera as an antioxidant source. Med. Pet. 38:169-175. https://doi.org/10.5398/medpet.2015.38.3.169
Kastelic, J. P. 2013. Male involvement in fertility and factors affecting semen quality in bulls. Animal Frontiers. 3: 20-25. https://doi.org/10.2527/af.2013-0029
Kumar, N., R. P. Verma, L. P. Singh, V. P. Varshney, & R. S. Dass. 2006. Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus × Bos taurus) bulls. Reprod. Nutr. Dev. 46:663–675. https://doi.org/10.1051/rnd:2006041
Lemma. A. & T. Shemsu. 2015. Effect of age and breed on semen quality and breeding soundness evaluation of pre-service young bulls. J. Reprod. Infertil. 6: 35-40.
Madukwe, E. U., J. O. Ezeugwu, & P. E. Eme. 2013. Nutrient composition and sensory evaluation of dry Moringa oleifera aqueous extract. Int. J. Sci. Basic Appl. Res. 13: 100-102.
Martin, G. B., D. Blache, D. W. Miller, &. E. Vercoe. 2010. Interactions between nutrition and reproduction in the management of the mature male ruminant. Animal 4:1214–1226. https://doi.org/10.1017/S1751731109991674
Menegassi, S. R. O., J. O. J. Barcellos, V. Peripolli, & C. M. Camargo. 2011. Behavioral assessment during breeding soundness evaluation of beef bulls in Rio Grande do Sul. Anim. Reprod. 8:77-80.
Moyo, B., P. J. Masika, A. Hugo, & V. Muchenje. 2011. Nutritional characterization of Moringa (Moringa oleifera Lam.) Leaves. Afr. J. Biotechnol. 10:12925-12933. https://doi.org/10.5897/AJB10.1599
Nouman, W., M. T. Siddiqui, S. M. A. Basra, H. Farooq, M. Zubair, & T. Gull. 2013. Biomass production and nutritional quality of Moringa oleifera as a field crop. Turk. J. Agric. For. 37: 410-419. https://doi.org/10.3906/tar-1206-29
Nouman, W., S. M. A. Basra, M. T. Siddiqui, A. Yasmeen, T. Gull, & M. A. C. Alcayde. 2014. Potential of Moringa oleifera L. as livestock fodder crop: a review. Turk. J. Agric. For. 38:1-14. https://doi.org/10.3906/tar-1211-66
Nutrient Requirements of Beef Cattle. 2000. Seventh Revised Edition: Update Subcommittee on Beef Cattle Nutrition, Committee on Animal Nutrition, National Research Council. Available from the National Academies Press at: http://www.nap.edu/catalog/9791.html.
Perumal, P., S. K. Srivastava, S. K. Ghosh, & K. K. Baruah. 2014. Computer-assisted sperm analysis of freezable and nonfreezable Mithun (Bos frontalis) semen. Journal of Animals 2014:Article ID 675031, 6 pages.
Prabsattroo, T., J. Wattanathorn, S. Iamsaard, S. Muchimapura, & W. Thukhammee. 2012. Moringa oleifera leaves extract attenuates male sexual dysfunction. Am. J. Neurosci. 3:17-24. https://doi.org/10.3844/amjnsp.2012.17.24
Prabsattroo, T., J. Wattanathorn, S. Iamsaard, P. Somsapt, O. Sritragool, W. Thukhummee, & S. Muchimapura. 2015. Moringa oleifera extract enhances sexual performance in stressed rats. J. Zhejiang Univ. Sci. B 16:179-190. https://doi.org/10.1631/jzus.B1400197
Princewill, O. I., A. E. Uchenna, O. I. Charles, & I. M. Uwaezuoke. 2015. Interactions between dietary minerals and reproduction in farm Animal. Global Journal of Animal Scientific Research 3:524-535.
Priyadarshani, N & M. C.Varma. 2014. Effect of Moringa oleifera leaf powder on sperm count, histology of testis and epididymis of hyperglycaemic mice Mus musculus. Am. Int. J. Res. Form. Appl. Nat. Sci. 7:07-13.
Rajanandh, M. G. & J. Kavitha. 2010. Quantitative estimation of β-sitosterol, total phenolic and flavonoid compound in the leaves of Moringa oleifera. Int. J. Pharmtech. Res. 2:1409-1414.
Raji, A. Y. & A. A. Njidda. 2014. Gonadal and extra-gonadal sperm reserves of the Red Sokoto goats fed Moringa oleifera supplemented diets. Inter. J. Agri. Biosci. 3:61-64.
Roy, B., R. P. S. Baghel, T. K. Mohanty, & G. Mondal. 2013. Zinc and male reproduction in domestic animals: A Review. Indian J. Anim. Nutr. 30:339-350.
Sarastina, T. Susilawati, & G. Ciptadi. 2012. Analysis of sperm motility parameters in various breed bulls using a computer-assisted semen analysis (CASA) (In Indonesian). J. Ternak Tropika 6:1-12.
Setchell, B. P. 2014. Semen and its Constituents. In: Chenoweth, P. J. & S. P. Lorton (Eds). Animal Andrology: Theories and Applications. CAB International, Boston, USA. p.3-11. https://doi.org/10.1079/9781780643168.0003
Susilawati, T. 2011. Spermatologi (In Indonesian). Universitas Brawijaya Press (UB Press), Malang.
Wu, G., F. W. Bazer, T. A. Davis, S. W. Kim, P. Li, J. M. Rhoads, M. C. Satterfield, S. B. Smith, T. E. Spencer, & Y. Yin. 2009. Arginine metabolism and nutrition in growth, health, and disease. Amino Acids 37:153-168. https://doi.org/10.1007/s00726-008-0210-y
Young, S. S., B. Eskenazi, F. M. Marchetti, G. Block, & A. J. Wyrobek. 2008. The association of folate, zinc and antioxidant intake with sperm aneuploidy in healthy non-smoking men. Hum. Reprod. 23:1014-1022. https://doi.org/10.1093/humrep/den036
Copyright (c) 2017 Media Peternakan
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.Authors submitting manuscripts should understand and agree that copyright of manuscripts published are held by Media Peternakan. The statement to release the copyright to Media Peternakan is stated in Form A. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.