Conception Rates of Holstein-Friesian Cows Inseminated Artificially with Reducing Frozen Semen Doses
Abstract
Conception rates of Holstein Friesian (HF) acceptors were evaluated in artificial insemination (AI) mating using three frozen semen doses of the straws per volume of 0.25 cc. Frozen semen consisted of a standard dose by 25 x 106 sperms (L.1) and two reduced doses by 20 x 106 sperms (L.2) and 15x106 sperms (L.3). This field study was conducted in dairy cattle area of Lembang NBMCU, from August 2011 to March 2012. Frozen semens were obtained from two HF national progeny tested young bulls. Conception was detected by rectal palpation, around 60-90 days after AI mating. Conception rates (CR %) were calculated as a ratio between pregnant acceptors to the total AI mated cows. Some general linear model analyzes were conducted by considering fixed variables of semen dose (3), bull (2), inseminator (6) and parity (5). The results showed that three frozen semen doses did not significantly affect (P>0.05) on CR (%). By decreasing semen dose to L.3 gave CR (%) similar to L2 even to L1. It was concluded that frozen semen doses of HF bulls at the levels of 20 million to 15 million sperms per straw per volume of 0.25 cc resulted CR (%) similar to the standard one.Downloads
References
Anderson, M., J. Taponen, E. Koskinen, & M. Dahlbom. 2004. Effect of insemination with doses of 2 or 15 million frozen-thawed spermatozoa and semen deposition site on pregnancy rate in dairy cows. Theriogenology 61: 1583-1588. http://dx.doi.org/10.1016/j.theriogenology.2003.09.006
Andrabi, S., M. H., M. Siddique, N. Ullah, & A. Khan. 2006. Effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. Pakistan Vet. J.. 2006. 26: 17-19.
Arifiantini, R. I., B. Purwantara, & M. Riyadhi. 2010. Occurence of sperm abnormality of beef cattle at several Artificial Insemination Centers in Indonesia. Anim. Prod. 12: 44-49.
Barszcz, K., D. Wiesetek, M. Wąsowicz, & M. Kupczyńska. 2012. Bull semen collection and analysis for artificial insemination. J. Agr. Sci. 4: 1-10.
Bhakat, M., T. K. Mohanty, V. S. Raina, A. K. Gupta, & H. M. Khan. 2011. Frozen semen production performance of murrah buffalo bulls. Buffalo Bulletin 30: 157-162.
Bucher, A., R. Kasimanickam, J. B. Hall, J. M. Dejarnette, W.
D. Whittier, W. Kähn, & Z. Xu Z. 2009. Fixed-time AI pregnancy rate following insemination with frozen- thawed or fresh-extended semen in progesteronesupplemented CO-Synch protocol in beef cows. Theriogenology 71: 1180-1185. http://dx.doi.org/10.1016/j.theriogenology.2008.12.009
Buckley, F., J. Mee, K. O’sullivan, R. Evans, D. Berry, & Patdillon. 2004. Insemination factors affecting the conception rate in seasonal calving Holstein-Friesian cows. Reprod. Nutr.Dev. 43: 543–555.
Das. B. C., S. Habib, M. Chowdhury, M. I. Hossain, & M. H. Islam. 2002. Qualitative changes of frozen bull smeen under field conditions in Bangladesh. Pakistan J. Biological Sci. 5: 481-483. http://dx.doi.org/10.3923/pjbs.2002.481.483
DeJarnette, J. M., C. R. McCleary, M. A. Leach, J. F. Moreno, R. L. Nebel, & C. E. Marshall. 2010. Effects of 2.1 and 3.5 × 106 sex-sorted sperm dosages on conception rates of Holstein cows and heifers. J. Dairy Sci. 93: 4079-4085. http://dx.doi.org/10.3168/jds.2010-3181
Drost, M. 2007. Bubaline versus bovine reproduction. Theriogenology 68: 447-449. http://dx.doi.org/10.1016/j.theriogenology.2007.04.012
Frijters, A. C. J., E. Mullaart, R. M. G. Roelofs, R. P. van Hoorne, J. F. Moreno, O. Moreno, & J. S. Merton. 2009. What affects fertility of sexed bull semen more, low sperm dosage or the sorting process?. Theriogenology 71:64-67. http://dx.doi.org/10.1016/j.theriogenology.2008.09.025
Herawati, T., A. Anggraeni, L. Praharani, D. Utami, & A. Argiris. 2012. Peran inseminator dalam keberhasilan inseminasi buatan pada sapi perah inseminator role in the success of artificial insemination on dairy cattle. Informatika Pertanian 21: 81-88.
INS (Standar Nasional Indonesia/SNI) 4869.1. 2008. Standar Semen Beku Sapi. http://sisni.bsn.go.id/index.php?/sni_main/sni/detail_sni/7026. Diakses tanggal 12 Desember 2013.
Januškauskas, A. & H. Žilinskas. 2002. Bull semen evaluation post-thaw and relation of semen characteristics to bull’s fertility. Veterinarija Ir Zootechnika. T. 17: 1-9.
Larson, J. E., G. C. Lamb, B. J. Funnell, S. Bird, A. Martins, & J. C. Rodgers. 2010. Embryo production in superovulated Angus cows inseminated four times with sexed-sorted or conventional, frozen-thawed semen. Theriogenology. 73:698-703. http://dx.doi.org/10.1016/j.theriogenology.2009.11.009
Lembang AIA (BIB Lembang). 2011. Proses Produksi Semen Beku. Buku Pintar Inseminasi Buatan. Balai Inseminasi Buatan (BIB) Lembang. Ditjen Peternakan dan Kesehatan Hewan. Kemen Pertanian, Lembang.
López-Gatius, F. 2012. Factors of a noninfectious nature affecting fertility after artificial insemination in lactating dairy cows: a review. Theriogenology 77:1029-1041. http://dx.doi.org/10.1016/j.theriogenology.2011.10.014
Muiño, R., A. I. Peña, L. A. Quintela, J. Becerra, P. Herradón, & F. H. Gonzale. 2015. Management of the storage of cryopreserved sperm on dairy cattle farms. Biotechnology in Animal Husbandry 31: 85-100. http://dx.doi.org/10.2298/BAH1501085M
Perry, M. J., S. A. Venners, X. Chen, X. Liu, G. Tang, H. Xing, D. B. Barr, & X. Xu. 2011. Organophosphorous pesticide exposures and sperm quality. Reprod. Toxicol. 31:75-79. http://dx.doi.org/10.1016/j.reprotox.2010.08.006
Sá Filho, M. F., H. Ayres, R. M. Ferreira, M. Nichi, M. Fosado, F. E. P. Campos, & P. S. Baruselli. 2010. Strategies to improve pregnancy per insemination using sex-sorted semen in dairy heifers detected in estrus. Theriogenology 74:1636–1642. http://dx.doi.org/10.1016/j.theriogenology.2010.06.036
Saha R., A. Ashraf, & Z. Rahman. 2014. Comparative Study on Conception Rate of Cow in Using Frozen and Liquid Semen. J. Anim. Sci. Adv. 4: 749-772.
Sarder, M. J. U. 2006. Study on the influence of sire on reproductive and productive capability of dairy cows used for artificial insemination programme at greater Rajshahi district. Rajshahi University Studies, Part-B. Journal of Science 34: 237-253.
Schenk, J. L., D. G. Cran, R. W. Everett, & G. E. Seidel. 2009. Pregnancy rates in heifers and cows with cryopreserved sexed sperm: Effects of sperm numbers per inseminate, sorting pressure and sperm storage before sorting. Theriogenology 71:717-728. http://dx.doi.org/10.1016/j.theriogenology.2008.08.016
Seidel, G. E. & J. L. Schenk. 2008. Pregnancy rates in cattle with cryopreserved sexed sperm: Effects of sperm numbers per inseminate and site of sperm deposition. Anim. Reprod. Sci. 105: 129-38. http://dx.doi.org/10.1016/j.anireprosci.2007.11.015
Shrivatava, N., M. K. Awasthi, S. B. Sahu, S. Sahastrabudhe, & R. P. Tiwa. 2013. Effect of Incubation Period at 30 0C and at 40 0C on Postthaw Characteristics of Frozen Semen of Hf Crossbred BullS. Indian Journal of Animal Reproduction 34: 22-25.
Copyright (c) 2016 Media Peternakan - Journal of Animal Science and Technology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors submitting manuscripts should understand and agree that copyright of manuscripts published are held by Media Peternakan. The statement to release the copyright to Media Peternakan is stated in Form A. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.