@article{Suharti_Aliyah_Suryahadi_2019, title={Karakteristik Fermentasi Rumen In vitro dengan Penambahan Sabun Kalsium Minyak Nabati pada Buffer yang Berbeda}, volume={16}, url={https://journal.ipb.ac.id/index.php/jurnalintp/article/view/24939}, DOI={10.29244/jintp.16.3.56-64}, abstractNote={<p>Canola oil and flaxseed oil are vegetable oils as potential sources of unsaturated fatty acid that could improve the production and quality of beef meat.&nbsp; However, the use of vegetable oils need to be protected to avoid biohydrogenation by rumen bacteria.&nbsp; The research was aimed to analyse effect of flaxseed/canola oils calcium soap in the different buffer media on in vitro fermentation characteristic.&nbsp; The experiment was conducted in a factorial randomized block design with 2 factors and 3 blocks based on rumen sampling time.&nbsp; The first factor was sources of vegetable oils (canola and falxseed) and the second factor was type of buffer (Kajikawa and McDougall).&nbsp; Variables observed were pH value, N-NH<sub>3</sub> concentration, total volatile fatty acid (VFA), dry matter and organic matter digestibility.&nbsp; Data obtained was analysed using Analysis of Variance and any significant different further tested using Duncan Multiple Range Test.&nbsp; The results showed that there was no interaction between sources of diet and buffer.&nbsp; Supplementation of canola and flaxseed oils protected by calcium soap at level 6% did not affect pH value, dry matter digestibility, rumen protozoa and total bacteria.&nbsp; The use of different buffers affected pH value, dry matter digestibility, rumen protozoa and total bacteria.&nbsp; It’s concluded that the used of flaxseed oil or canola oil calcium soap did not alter rumen fermentation and McDougall buffer could improve in vitro fermentation activity compare to Kajikawa buffer.</p> <p><strong>Keywords</strong>: biohydrogenation, calcium soap, canola oil, flaxseed oil, rumen fermentation</p&gt;}, number={3}, journal={Jurnal Ilmu Nutrisi dan Teknologi Pakan}, author={Suharti, S and AliyahD N and Suryahadi, S}, year={2019}, month={Jan.}, pages={56-64} }