GLUCOSE AND CORTISOL VARIATIONS: PRELIMINARY STUDY ON PHYSIOLOGICAL RESPONSE OF MUD CRAB AFTER TRANSPORTATION ACTIVITY

VARIASI GLUKOSA DAN KORTISOL: STUDI AWAL RESPONS FISIOLOGIS KEPITING BAKAU SETELAH KEGIATAN TRANSPORTASI

Abstract

The system of transporting mud crabs (Scylla spp.), carried out by the community by placing them in styrofoam boxes, was suspected of triggering stress in the biota. This study aims to determine the glucose and cortisol variation values of mud crabs as physiological response of the biota after transportation activity. Analysis was carried out on 2 groups of mud crab populations (I, II). Each population was observed 3 times, the crab had just arrived from the field (H₀), had been cultivated for 5 days (H₅), and had been cultivated for 10 days (H₁₀) in a plastic box (30x25x20 cm³) in cement pond (180x180x75 cm³) filled with seawater (25 PSU). Crabs were fed with fish pieces twice per day. In population I, the average glucose and cortisol values of H₀ ranged 78.3±30.5 mg/100 ml and 0.5±0.1 ng/dL, H₅ ranged 42.7±4.9 mg/100 ml and 1.0±0.3 ng/dL, and H₁₀ ranged 47.0±8.5 mg/100 ml and 0.6±0.2 ng/dL, respectively. In population II, H₁ ranged 60.4±37.4 mg/100 ml and 0.5±0.2 ng/dL, H₅ ranged 44.2±3.6 mg/100 ml and 0.9±0.3 ng/dL, and H₁₀ ranged 75.2±19.7 mg/100 ml (glucose). Based on these observations, the glucose values in the two mud crab populations varied (3.6-37.4 mg/100 ml), and according to the ANOVA test, they are insignificantly different (p>0.05). However, there is a downward trend from H₀ to H₅.