@article{Asnani_Rahayu_Jenie_Yuliana_2017, title={AKTIVITAS ANTIBAKTERI DAN SITOTOKSISITAS EKSTRAK DAUN KEDONDONG HUTAN}, volume={28}, url={https://journal.ipb.ac.id/index.php/jtip/article/view/17143}, DOI={10.6066/jtip.2017.28.2.169}, abstractNote={<p><em>Wild mango (S</em><em>pondias</em><em> pinnata) leaves is a very popular herb in Southeast Sulawesi, has been used as herbal medicine </em><em>at</em><em> </em><em>S</em><em>outhern and </em><em>S</em><em>outheastern Asia.</em><em> </em><em>The purpose of this study was to determine the antibacterial activity, cytotoxicity and phytochemical contents of wild mango (S</em><em>pondias</em><em> pinnata) leaves extracts. The extraction was performed using a combination of maceration with ethanol and ultra-sonication. The antibacterial activity assay of the extracts was examined against Staphylococcus aureus, Listeria monocyt</em><em>o</em><em>genes, </em><em>Klebsiella pneumoniae, Morga-nella morganii, <em>Pseudomonas aeruginosa, Shigella sonnei</em></em>,<em> Escherichia coli, and </em><em>Salmonella Typhi</em><em>. The cytotoxicity test was performed on Vero cells using</em><em> </em><em>3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium</em><em> </em><em>bromide (MTT)</em><em> </em><em>assay.</em><em> </em><em>The extracts were able to inhibit all tested bacteria with inhibition zone ranging from 4.48 to 7.49 mm. P. <em>aeruginosa</em> </em><em>was</em><em> the most susceptible bacterium, producing inhibition zone of 7.49 mm and a MIC </em><em>(minimum inhibition concentration) </em><em>value of 6.25 mg/mL. The extract was not toxic to Vero cells as indicated by IC<sub>50 </sub></em><em>(</em><em>inhibitory</em><em> concentration </em><em>of </em><em>50</em><em>%</em><em>)</em><em> values at 3</em><em>47 µg/mL</em><em>, or more than the toxicity limit of 50 µg/mL. Phytochemical analysis of the extract revealed that it contained steroids, tannins, and saponins. The flavonoids concentration was 23.2±0.33 mg/g and while the phenolic substance was 43.2±0.45 mg/g. The FTIR analysis showed </em><em>O-H, C-H, X=C=Y</em><em>,</em><em> C=O, C=C, </em><em>and</em><em> C-O-C</em><em> as the functional groups in the extracts</em><em>.</em><em> This result suggests that the ethanol extract of S. pinnata leaves has the potential to be used as natural preservatives in foods.</em></p&gt;}, number={2}, journal={Jurnal Teknologi dan Industri Pangan}, author={Asnani, Asnani and RahayuWiniati P. and JenieBetty Sri Laksmi and YulianaNancy D.}, year={2017}, month={Dec.}, pages={169-179} }