Estimating Chlorophyll and N Content in Corn Leaves Based on Chlorophyll Content Index
Estimasi Kadar Klorofil Dan Kadar N Daun Jagung Menggunakan Chlorophyll Content Index
Abstract
The chlorophyll content is related to the condition of the plant so that it can be used to estimate nutrient content in plants. The conventional measurement of leaf chlorophyll content is time consuming and expensive, so a fast and efficient technology is needed. This study aims to (a) analyze the chlorophyll content index (CCI) and leaf chlorophyll content in 10 fertilizer dosage treatments including control and several growth phases of corn, (b) analyze the relationship between CCI and leaf chlorophyll content, and (c) analyze the relationship between CCI and N content. In this study, the CCI was measured with a chlorophyll meter, while the chlorophyll content and N content of leaves were measured using a spectrophotometer and the Kjehdahl method, respectively. The relationship between CCI on chlorophyll content and N content was analyzed at 4 and 8 weeks after planting (WAP) using a simple linear regression model. The results showed that the CCI curve pattern in all dosage treatments was similar as indicated by the CCI increasing with increasing plant age and then decreasing with plant aging. Fertilizer dose had a significant effect on CCI except at 2 and 3 WAP, where the highest CCI was found at fertilizer doses with high nutrient adequacy, i.e. 2 Ultra Gen (UG) + 2/3 Standard (STD) at 7 MST. The CCI and chlorophyll content had a positive linear relationship at 4 WAP and 8 WAP with a strong (r = 0.67) and very strong (r = 0.76) correlation. The relationship model between CCI and leaf N content showed a very strong positive correlation at 8 WAP with the equation N (%) = 0.019*CCI + 1.795 (r = 0.75). These results indicate that the measurement of the CCI with a chlorophyll meter can be used to estimate the N nutrient status of plant leaves properly.
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Department of Soil Science and Land Resources Departemen Ilmu Tanah dan Sumberdaya Lahan, Faculty of Agriculture Fakultas Pertanian, IPB University