Some types of seaweed are reported to have antidiabet activity in vivo or in vitro,
but the activity antidiabet fractions of polysaccharides from seaweed has not been widely
reported. Gracilaria gigas is one type of red seaweed that can produce agar and it has two
factions, namely agarose and agaropectin. The aim of this study was to obtain an agar
extract, agarose fraction, agaropektin fraction of Gracilaria gigas and to determine the
α-glucosidase inhibitory activity of extracts and the fractions. Extraction of agar that used
an ethanol solution, and to fractionate agarose and agaropektin used dimetilsulfoxide
solution. The results showed that the fraction of the agarose having lower sulfate content
(0.28%) compared with agar (5.91%) and agaropektin (6.07%). Types of samples (agar,
agarose, and agaropektin) and sample doses significantly in inhibiting α-glucosidase
enzyme activity. Agarose fraction has IC50 value against α-glucosidase lowest (96.86 ± 4.61
ppm), followed by the extract agar (116.63 ± 5.61 ppm), then the fraction agaropektin
(158.34 ± 1.77 ppm).
Keywords: α-glucosidase, extract, fraction, Gracilaria gigas, IC50
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