@article{Husain_Surdaya_Purnomo_2022, title={Mutation Induction using Colchicine in Shallot Bulbs (Allium ascalonicum L.) of Tajuk Variety}, volume={13}, url={https://journal.ipb.ac.id/index.php/jhi/article/view/39077}, DOI={10.29244/jhi.13.1.1-7}, abstractNote={<p><span id="page1196R_mcid32" class="markedContent"><span dir="ltr" role="presentation">Techniques in the fold of shallot plant breeding can be done to obtain or improve existing characters to be better than be</span></span><span id="page1196R_mcid34" class="markedContent"><span dir="ltr" role="presentation">fore. One of them is the mutation induction technique with a chemical mutagen, namely colchicine. The purpose of the study was </span></span><span id="page1196R_mcid35" class="markedContent"><span dir="ltr" role="presentation">to find out the effect of colchicine concentration on growth, yield, character diversity and obtain value of Lethal Concentration </span></span><span id="page1196R_mcid36" class="markedContent"><span dir="ltr" role="presentation">20-50% (LC</span></span><span id="page1196R_mcid37" class="markedContent"><span dir="ltr" role="presentation">20-50</span></span><span id="page1196R_mcid38" class="markedContent"><span dir="ltr" role="presentation">) for Tajuk Vriety M1 of shallot. The research was conducted in July-October 2019 in Libuo Village of Dungingi </span></span><span id="page1196R_mcid39" class="markedContent"><span dir="ltr" role="presentation">District of Gorontalo City. The study was conducted using a design randomized block design (RBD) with six level of colchicine </span></span><span id="page1196R_mcid40" class="markedContent"><span dir="ltr" role="presentation">solution concentration (0, 0.1, 0.2, 0.3, 0.4 and 0.5% (w/v)) with three repeats. The results of the variety analysis showed that a </span></span><span id="page1196R_mcid41" class="markedContent"><span dir="ltr" role="presentation">solution of colchicine concentration of 0-0.5% had the effect on the morphology of leaf colour, the number of saplings and the </span></span><span id="page1196R_mcid42" class="markedContent"><span dir="ltr" role="presentation">percentage of seeds growing abnormally and character of lear colour shallot varieties of mutant putative generation M1, but had </span></span><span id="page1196R_mcid43" class="markedContent"><span dir="ltr" role="presentation">no significantly effect on plant height, number of bulbs, wet and dry weights. CurveExpert1.3 analysis result, the lethal concen</span></span><span id="page1196R_mcid45" class="markedContent"><span dir="ltr" role="presentation">tration value of colchicine solution in shallot bulbs can be obtained at a concentration of 0.33 (w/v) at LC</span></span><span id="page1196R_mcid46" class="markedContent"><span dir="ltr" role="presentation">30</span></span><span id="page1196R_mcid47" class="markedContent"><span dir="ltr" role="presentation">.</span></span></p> <p><span id="page1196R_mcid47" class="markedContent"><span dir="ltr" role="presentation"><br></span></span><span id="page1196R_mcid48" class="markedContent"><span dir="ltr" role="presentation">Keywords: abnormal, LC</span></span><span id="page1196R_mcid49" class="markedContent"><span dir="ltr" role="presentation">30</span></span><span id="page1196R_mcid50" class="markedContent"><span dir="ltr" role="presentation">, LC</span></span><span id="page1196R_mcid51" class="markedContent"><span dir="ltr" role="presentation">50</span></span><span id="page1196R_mcid52" class="markedContent"><span dir="ltr" role="presentation">, mutagen, putative mutant</span></span></p&gt;}, number={1}, journal={Jurnal Hortikultura Indonesia}, author={Husain, Indriati and SurdayaTedy and PurnomoSutrisno Hadi}, year={2022}, month={Apr.}, pages={1-7} }