Pengembangan Metode Uji Cepat Vigor Benih Kacang Panjang (Vigna sinensis L.) melalui Pemunculan Radikula menggunakan Pengolahan Citra Digital

The Development of a Rapid Seed Vigor Testing Method for Long Bean (Vigna sinensis L.) through Radicle Emergence utilizing Digital Image Processing

Abstract

Radicle emergence test represents a promising alternative method for rapid seed vigor assessment. Digital image processing stands as a technology viable for seed quality identification through a more precise and time-efficient radicle emergence method. The research endeavors to develop a more efficient and accurate method for testing seed vigor via radicle emergence utilizing digital image processing on Long Bean (Vigna sinensis L.) seeds. Conducted at the Seed Physiology and Health Laboratory, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University, from October 2023 to January 2024, the study employed a completely randomized design with one factor and four replications as groups. Ten varieties of Long Bean seeds were utilized, and radicle emergence assessments were conducted from 46 to 58 hours after sowing. The findings indicate that digital image processing can effectively be utilized in radicle emergence assays for Long Bean seed vigor testing. Radicle emergence test exhibited a strong positive correlation with germination rate, vigor index, and growth rate while showing a strong negative correlation with the average germination time. The most suitable and accurate germination period for a radicle emergence test of at least 2 mm is 52 hours at 25 ± 2 ℃. Radicle emergence assay proves reliable and efficient for testing Long Bean seed vigor, particularly when integrated with digital image processing technology.

Keywords: correlation, growth rate, ImageJ, salinity stress, vigor index