The Potential of White Tea (Camellia sinensis) and Kelor (Moringa oleifera) in Improving Lipid Profile and Histopathological Features of Pancreas in Streptozotocin-Induced Rats

Rina Martini, Amalia Rahma, Clara Meliyanti Kusharto, Hadi Riyadi, Cece Sumantri, Dadan Rohdiana

Abstract

This study aimed to examine the potential of white tea and kelor in improving lipid profile and histopathological features of the pancreas in streptozotocin-induced Sprague-Dawley rats. The research design was an experimental study with post-test control group design. Catechin content were analysed using High-Performance Liquid Chromatography. Twenty Sprague-Dawley rats (out of 24 rats, 12-week old, 200-300 g) were induced with intraperitoneal injection of streptozotocin at a dose of 40 mg/kg BW. Four groups of rats received one of the green tea (GT), white tea (WT), kelor (K), or the mixture of white tea and kelor (WTK) with a dose of epigallocatechin-3-gallate (EGCG) at 100 mg/kg BW administered orally for 21 days. Measurements of the lipid profile and preparation of histopathological features of the pancreas were performed after the intervention was completed. The results showed that white tea had the highest concentrations of catechin (39.17%), gallic acid (1.09%), EGCG (4.46%), and epicatechin (9.61%) compared to the other tea groups. The TG levels of the WTK group (95±19.35 mg/dl) were significantly different (p<0.05) from the WT and K groups but not significantly different from the normal (105.8±23.89 mg/dl), DM and GT groups. Meanwhile, the HDL levels did not show significant differences in each intervention group (p>0.05). The mean diameter of the islets of Langerhans in the DM group (9.16± 2.56 μm) was significantly different (p<0.05) from the WT (20±8.94 μm), K (17.16±5.26 μm), WTK (18.66±4.17 μm), and N (21.07±8.49 μm) groups but not significantly different from the GT group (14.33±5.24 μm). The histopathological features of the pancreas showed an increase in the diameter of the islets of Langerhans in WT, K, and WTK groups. This study revealed that the mixture of white tea and kelor had the potential to ameliorate triglyceride levels and histopathological features of the pancreas in streptozotocin-induced Sprague-Dawley rat.

References

Abdulkadir A, Zawawi D, Jahan S. 2015. DPPH antioxidant, total phenolic and total flavonoid content of different part of drumstick tree (Moringa oleifera). J Chem Pharm Res 7(4):1423-1428.

Afify AEMR, Shalaby EA, El-Beltagi HS. 2011. Antioxidant activity of aqueous extracts of different caffeine products. Not Bot Horti Agrobo 39(2):117-123.

Alipoor B, Homayouni A, Mehrabani V. 2012. Diabetes, Oxidative Stress and Tea. Environmental Induction and Dietary Antioxidants. http://www.intechopen.com.

Ambarwati, Sarjadi, Andrew J, Kis D. 2014. Efek Moringa oleifer terhadap gula darah dan kolagen matrik ekstraseluler sel pancreas diabetes eksperimental. Kedokteran Brawijaya 28(2):74-78.

Banjarnahor E, Sunny W. 2012. Sel beta pankreas: sintesis dan eksresi insulin. J Biomedik 4(3):156-162.

Berenbaum MC. 1989. What is synergy? Pharmacol Rev 41(2):93-141.

Chaplin S. 2016. NICE guidance on managing type 2 diabetes in adults. Prescriber 27(4):22-29.

Che CT, Wang ZJ, Chow MSS, Lam CWK. 2013. Herb-herb combination for therapeutic enhancement and advancement: theory, practice and future Perspectives. Molecules 18(5):5125-5141.

Divi SM, Bellamkonda R, Dasireddy S.K. 2012. Evaluation of antidiabetic and antihyperlipidemic potential of aqueous extract of Moringa oleifera in fructose fed insulin resistant and STZ induced diabetic Wistar rats: a comparative study. Asian J Pharm Clin Res 5(1):67-72.

Effendi A. 2013. Nutrigenomik Resistensi Insulin Sindrom Metabolik Prediabetes. Bogor: IPB Press.

Geleta B, Makonnen E, Debella A, Tadele A. 2016. In vivo antihypertensive and antihyperlipidemic effects of the crude extracts and fractions of Moringa stenopetala (Baker f.) Cufod. Leaves in rats. Front. Pharmacol 7:(97):1-10.

Gopalakrishnan L, Doriya K, Kumar D. 2016. Moringa oleifera: A review on nutritive importance and its medicinal application. J Food Sci Hum Well 5(2):49-56.

Gupta D, Bhaskar D, Gupta R, Karim B, Jain A, Dalai D. 2014. Green tea: A review on its natural anti-oxidant therapy and cariostatic benefits. J Pharm Biol Sci 2(1):8-12.

Hanhineva K, Torronen R, Bondia P, Pekkinen J, Kolehmainen M, Mykkanen H, Poutanen K. 2010. Impact of dietary polyphenols on carbohydrate metabolism. 2010. Int J Mol Sci 11(4):1365-1402.

Hemant U, Pradip S, Tarannum P. 2014. A study on the effects of Moringa oleiferalam. pod extract on alloxan induced diabetic rats. Asian J Plant Sci 4(1):36-41.

Hilal Y, Engelhardt U. 2007. Characterisation of white tea comparison to green and black tea. J Verbrauch Lebensm 2(4):414-421. Islam SMd. 2011. Effects of the aqueous extract of white tea (Camellia sinensis) in a stretozotocin-induced diabetes model of rats. Phytomedicine 19(1):25-31.

Jigisha A, Nishant R, Navin K, Pankaj G. 2012. Green tea: a magical herb with miraculous 334 outcomes. Int J Pharm Sci Res 3(5):139-148.

Kim H, Michael J, Jeong K. 2014. New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate. Redox Biol 2(2014):187-195.

Malki A, Rabey H. 2015. The Antidiabetic effect of low doses of Moringa oleifera Lam seeds on streptozocin induced diabetes and diabetic nephropathy in male rats. Biomed Res Int 2015(2015):1-13.

Martono Y dan Martono S. 2012. Analisis kromatografi cair kinerja tinggi untuk penetapan kadar asam galat, kafein dan epigalokatekin galat pada beberapa produk teh celup. Agritech 32(4):362-369.

Mbikay. 2012. Theurapetic potential of Moringa oleifera leaves in choric hyperglycemia and dyslipidemia: a review. Front Pharmacol 3(24):1-12.

Noor A, Gunasekaran S, Vijayalakshmi M. 2017. Improvement of insulin secretion and pancreatic β-cell function in streptozotocin induced diabetic rats treated with Aloe vera extract. Pharmacognosy Res 9(1):99-104.

Park JH, Bae JH, Im SS, Song DK. 2014. Green tea and type 2 diabetes. Integr Med Res 3(2014):4-10.

Rahma A, Martini R, Kusharto C, Damayanthi E, Rohdiana D. 2017. Teh putih (Camellia sinensis) dan kelor (Moringa oleifera) sebagai antihiperglikemi pada tikus Sprague dawley yang diinduksi streptozotocin. J Gizi Pangan 12(3):179-186.

Reygaert WC. 2014. The Antimicrobial Possibilities of Green Tea. Frontiers in Microbiology. https://www.frontiersin.org/articles/10.3389/fmicb.2014.00434/full.

Roghani M, Baluchnejadmojarad T. 2010. Hpoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiol 17(1):55-59.

Rohdiana, D. 2015. Teh, proses karakteristik dan komponen fungsional. Food Rev Ind 9(8):34-37.

Szkudelski T. 2001. The mechanism of alloxan and streptozotocin action in β cells of rats pancreas. Physiol Res. 50(6):536-546.

Teixeira LG, Lages PC, Jascolka TL, Aguilar EC, Soares FL, Pereira SS, Beltrao NR, Matoso RO. 2012. White tea (Camellia sinensis) extract reduces oxidative stress and triacylglycerols in obese mice. Cienc Tecnol Aliment 32(4):733-741.

Tsao R. 2010. Chemistry and biochemistry of dietary polyphenols. Nutrients 2(12):1231-1246.

Wagner H. 2011. Synergy research: approaching a new generation of phytopharmaceuticals. Fitoterapia 82(2011):34-37.

Wagner H, Mezernich GU. 2009. Synergy research: approaching a new generation of phytopharmaceuticals. Phytomed 16(2009):97-110.

Wardani E, Sunaryo H, Sopiani MZ, Fatahillah M. 2015. Aktivitas antihypertrigliserida dan antihiperglikemik ekstrak daun kelor (Moringa oleifera Lam.) pada tikus hipergliserida diabetes. Media Farmasi 12(2):199-212.

[WHO] World Health Organization. 2016. Global report on diabetes. WHO Library Cataloguing-in-Publication Data.

Wikanta T, Kristi D, Rahayu L, Fajarningsih D. 2011. Aktivitas antihiperglikemia ekstrak etanol Turbinaria decurrens. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan 6(2):147-156.

Wolfram S, Raederstorff D, Preller M, Wang Y, Teixeira SR, Riegger C, Weber P. 2006. Epigallocatechin gallate supplementation alleviates diabetes in rodents. J Nutr 136(10):2512–2518.

Yun S, Kim S, Song D. 2006. Effects of (-)epigallocatechin-3-gallate on pancreatic beta-cell damage in streptozotocin-induced diabetic rats. Eur J Pharmacol 541(1-2):115-121.

Authors

Rina Martini
rinamartini99@yahoo.co.id (Primary Contact)
Amalia Rahma
Clara Meliyanti Kusharto
Hadi Riyadi
Cece Sumantri
Dadan Rohdiana
MartiniR., RahmaA., KushartoC. M., RiyadiH., SumantriC., & RohdianaD. (2019). The Potential of White Tea (Camellia sinensis) and Kelor (Moringa oleifera) in Improving Lipid Profile and Histopathological Features of Pancreas in Streptozotocin-Induced Rats. Jurnal Gizi Dan Pangan, 14(1), 23-30. https://doi.org/10.25182/jgp.2019.14.1.23-30

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