Konjugat Poliklonal Antibodi Nanopartikel Emas untuk Deteksi Potato Virus Y
Abstract
Polyclonal Antibodi-Gold Nanoparticles for Potato Virus Y Detection
Gold nanoparticles are stable colloidal solutions with dimensions of 1-100 nm having surface plasmon resonance with six free electrons. The existing of six free electrons on the surface of a plasmon causes gold nanoparticles to bind easily to various types of bioreseptors including polyclonal antibodies. Polyclonal potato virus Y (PVP) antibodi been successfully conjugate with gold nanoparticles in order to develop a rapid detection for PVY infection in potato plants. The gold nanoparticles was synthetized by the reduction of gold (III) chloride trihydrate (HAuCl4) with 1% sodium citrate. Subsequently, the nanoparticles were used to make gold nanoparticle-antiobody PVY-conjugate. PVY detection was carried out with dot blot method on the nitrocellulose membrane. The results showed that the PVY virus on the membrane can be detected 10-30 minutes after incubation, depend on the concentration of the conjugate and the concentration of the virus in the sampel. The use of gold nanoparticle conjugates can increase the efficiency of the immunodot blot method in about 1 hour, and this method can be developed to be a lateral flow system for field detection of PVY.
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References
Cao B, Xi T, Hiu D. 2011. Preparation of nanogold labeled goat-anti-rabbit IgG and their application in an immunoassay. Metalurgija-MJoM. 17(2):79–85.
Eustis S, El-Sayed M. 2005. Aspect ratio dependence of the enhanced fluorescence intensity of gold nanorods: experimental and simulation study. J Phys Chem B. 109 (34):16350–16356. DOI: https://doi.org/10.1021/jp052951a.
Friendland RP, Tedesco JM, Wilson AC, Atwood CS, Smith MA, Perry G, Zagorski MG. 2008. Antibodies to Potato virus Y bind the Aβ peptide: Immunohistochemical and NMR studies. J Biol Chem. 10: 1-16. DOI: https://doi.org/10.1074/jbc.M802088200.
Hussain A, Arif M, Abbas A, Hussain B, Ali M, Jaffar S. 2016. A review on aphid-borne virus (Potato virus Y). JEZS. 4(3):189-192.
Ljungblad J. 2009. Antibodi-conjugated gold nanoparticles integrated in a fluorescence based biochip. [thesis]. Linkoping (SE): Linkopings Universitet.
Link S, El-Sayed MA. 1999. Size and temperature dependence of the plasmon absorption of colloidal gold nanoparticles. J Physic Chem B. 103:4212–4217. DOI: https://doi.org/10.1021/jp984796o.
Parida UK, Bindhani BK, Nayak P. 2011. Green synthesis and characterization of gold nanoparticles using onion (Allium cepa) extract. World J Nano Sci Engineering. 1:93–98. DOI: https://doi.org/10.4236/wjnse.2011.14015.
Pham VD, Hoang H, Phan TH, Conrad U, Chu HH. 2012. Production of antibodi labeled gold nanoparticles for influenza virus H5N1 diagnosis kit development. Adv Nat Sci. 3:1–7. DOI: https://doi.org/10.1088/2043-6262/3/4/045017.
Razak KA, Makhsin SR, Zakaria ND, Nor NM. 2016. Chapter 2.6. Gold nanoparticles for diagnostic development. Di dalam: Ismail A. Nor NM, Abdullah JM, Acosta A, Sarmiento ME (editor). Sustainable Diagnostics for Low Resource Areas. Kuala Lumpur (MY): Universiti Sains Malaysia.
Sambrook J, Russell DW. 2001. Molecular Cloning: A laboratory manual. 3rd ed. New York (US):Cold Spring Harbor Laboratory Press.
Sapsford KE, Algar WR, Berti L, Gemmill KB, Casey BJ, Oh E, Stewart MH, and Medintz IL. 2013. Functionalizing nanoparticles with biological molecules: developing chemistries that facilitate nanotechnology. Chemical Reviews. 113(3):1904–2074. DOI: https://doi.org/10.1021/cr300143v.
Schramm S, Frost K, Charkowski A, Gray S, Crockford A, Groves RL. 2011. Management of Potato virus Y (PVY) in Wisconsin seed potato production. [graduated research]. Madison (US): University of Wisconsin.
Tripathi K, Driskell JD. 2018. Quantifying bound and active antibodies conjugated to gold nanoparticles: a comprehensive and robust approach to evaluate immobilization chemistry. ACS Omega. 2018(3):8253−8259. DOI: https://doi.org/10.1021/acsomega.8b00591.
Tyagi H, Kushwaha A, Kumar A, Aslam M. 2016. A facile pH controlled citrate-based reduction method for gold nanoparticle synthesis at room temperature. NRL. 11(362):1–11. DOI: https://doi.org/10.1186/s11671-016-1576-5.
Yeh CS, Cheng FY and Huang CC. 2012. Bioconjugation of noble metal nanoparticles and their applications to biolabeling and bioimaging. Di dalam: Chau LK dan Chang HT, editor. From Bioimaging to Biosensors: Noble Metal Nanoparticles in Biodetection. California (US): Pan Stanford Publishing Pte. Ltd. Hlm 11–28. DOI: https://doi.org/10.1201/b13162-3.
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