Analisis Keragaman Genetik Isolat Bakteri Xanthomonas oryzae pv. oryzae dari Jawa Barat dan Jawa Tengah Berdasarkan Analisis ARDRA Gen 16SrRNA

  • Yadi Suryadi Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor
  • Dwi N. Susilowati Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor
  • Puji Lestari Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor
  • Sutoro Sutoro Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor
  • Ifa Manzila Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian, Bogor
  • Trini Suryani Kadir Balai Besar Penelitian Tanaman Padi, Subang
  • Sheryn Sunni Albani Institut Pertanian Bogor
  • I Made Artika Institut Pertanian Bogor
DOI: https://doi.org/10.14692/jfi.10.2.53

Abstract

Fifteen Xanthomonas oryzae pv. oryzae isolates from several regions in West Java and Central Java have been studied for their genetic diversity using amplified ribosomal DNA restriction analysis (ARDRA) 16SrRNA gene.Total of 13 ARDRA patterns from digestion of DNA with RsaI restriction enzyme was obtained, indicated higher genetic diversity of X. oryzae pv. oryzae. Phylogenetic analysis revealed a close genetic relationships among isolates. Isolates X. oryzae pv. oryzae 1/96pml and 61pml are closely related with isolates 29d, 59pml, and 60pml although they are isolated from different locations. The other closely related isolates were between X. oryzae pv. oryzae 5mgl with 23d, 38d, 10sbg, 8myd; while 6klt was close to 3ind and 2kr. The result of X. oryzae pv. oryzae genetic diversity can be used as a reference for strain or/isolates selection for development ofbacterial leaf blight resistance in Indonesia.

 

Downloads

Download data is not yet available.
Published
2014-08-21
How to Cite
SuryadiY., SusilowatiD. N., LestariP., SutoroS., ManzilaI., KadirT. S., AlbaniS. S., & ArtikaI. M. (2014). Analisis Keragaman Genetik Isolat Bakteri Xanthomonas oryzae pv. oryzae dari Jawa Barat dan Jawa Tengah Berdasarkan Analisis ARDRA Gen 16SrRNA. Jurnal Fitopatologi Indonesia, 10(2), 53. https://doi.org/10.14692/jfi.10.2.53
Issue
Vol. 10 No. 2 (2014)
Section
Articles

Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:

1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.

3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.