Penyakit Mosaik Kuning Pada Kedelai
Abstract
Yellow mosaic Disease on Soybean
Epidemic of yellow mosaic disease had occurred in yard long bean (Vigna unguiculata subsp. sesquipedalis) in Java in 2008. However, this disease has never been reported in other areas in Indonesia. Observations on soybean plants in Cirebon (West Java), Bantul (DI Yogyakarta), and Musi Banyuasin (South Sumatra) showed systemic symptoms of yellow mosaic on leaves with vein banding. Plant samples showing yellow mosaic symptoms were taken for virus detection using PCR (polymerase chain reaction). The amplification results with specific MYMIV primers showed 1000 bp DNA bands. The results of analysis nucleotide sequence homology showed MYMIV Cirebon has a high homology (98.6-99.2 %) with MYMIV from Tegal, Subang, Klaten, Magelang, Brebes, and Bogor. This confirmed that the yellow mosaic symptom leaf samples from Bantul, Musi Banyuasin, and Cirebon were positively infected with MYMIV. Phylogenetic analysis shows that the Cirebon MYMIV isolates formed a group with all MYMIV sequences in Indonesia and Oman, and separated from MYMIV groups from other countries namely Bangladesh, Nepal, Pakistan and India. MYMIV Cirebon isolate was registered in GenBank with accession number LC481009. MYMIV has been reported in soybean in Java (2013). This finding is the first report of MYMIV infection in soybeans in South Sumatera.
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References
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