https://journal.ipb.ac.id/index.php/jai/issue/feed Jurnal Akuakultur Indonesia 2020-05-05T10:41:09+07:00 Prof. Dr. Muhammad Zairin Junior akuakultur.indonesia@gmail.com Open Journal Systems <p>Jurnal Akuakultur Indonesia (JAI) is a scientific journal publishes empirical research and recent science development in aquaculture. JAI is published twice a year in January and July since 2002 by the Indonesian Society of Scientific Aquaculture (ISSA) in association with the Department of Aquaculture, Faculty of Fisheries and Marine Science, Bogor Agricultural University. JAI accepts manuscripts written in English and in Bahasa (Indonesian). JAI only processes submitted original script related to aquaculture and not being published by other publishers. JAI has been registered in some indexing tools like Directory of Open Access Journals (DOAJ), Indonesian BASE, Publication Index (IPI), Google Scholar, Portal Garuda, Mendeley, SINTA, and IPB repository.</p> <p>&nbsp;</p> <p>p-ISSN <a href="http://u.lipi.go.id/1180429182" target="_blank" rel="noopener">1412-5269</a>&nbsp; /&nbsp; e-ISSN <a href="http://u.lipi.go.id/1385091088" target="_blank" rel="noopener">2354-6700</a></p> https://journal.ipb.ac.id/index.php/jai/article/view/29256 Characterization of fermentation liquid from mangrove leaves Avicennia marina and its inhibitory potential for bacterium causing ice-ice disease 2020-01-24T02:43:16+07:00 Samsu Adi Rahman jcbanggai@gmail.com Sukenda sukenda@ipb.ac.id Widanarni widanarni@yahoo.com Alimuddin alimuddin.alsani@gmail.com Julie Ekasari jekasari@gmail.com <p><strong>ABSTRACT</strong></p> <p>&nbsp;</p> <p>Fermentation liquid from mangrove leaves <em>Avicennia marina</em> contains microorganisms, nutrients, and secondary metabolites. This study aimed to identify bacteria and the compounds in fermentation liquid of mangrove leaves <em>A. marina</em> and measured their inhibitory capacity against pathogenic bacteria <em>Stenotrophomonas maltophilia</em> which causes ice-ice disease in seaweed. Molecular analysis which aimed the 16S rRNA gene showed that the bacteria in fermentation liquid consisted of eight types of <em>Bacillus</em>, <em>Bacillus subtilis</em> MSAR-01, <em>Bacillus megaterium</em> MSAR-02, <em>Bacillus firmus</em> MSAR-03, <em>Bacillus thuringiensis</em> MSAR-04, <em>Bacillus subterranerus</em> MSAR-05, <em>Bacillus vietnamensis</em> MSAR-06, <em>Bacillus</em> sp. MSAR-07,<em> Bacillus circulans</em> MSAR-08, with the best inhibitory power indicated by <em>B. subtilis</em> MSAR-01, <em>B. vietnamensis</em> MSAR-06, and <em>Bacillus</em> sp. MSAR-07. The administration of lactic acid, bacteriocin, total fermentation liquid, and supernatant as much as 15 mL produce inhibition to <em>S. maltophilia</em> indicated better result &nbsp;than using one or a combination of several types of bacterial isolates. The inhibition of single bacterial enriched fermentation and supernatant liquids was better than bacterial combination enrichment.</p> <p>&nbsp;</p> <p>Keywords: <em>Avicennia marina</em>, fermentation, ice-ice, mangrove</p> 2020-01-24T02:17:15+07:00 Copyright (c) 2020 Jurnal Akuakultur Indonesia https://journal.ipb.ac.id/index.php/jai/article/view/29710 Reproductive and growth performances in female giant freshwater prawn following inhibition of gonadal maturation using dopamine and medroxyprogesterone hormone 2020-02-18T07:26:15+07:00 Megawati Wijaya megawatiwijaya20@gmail.com Agus Oman Sudrajat omanipb@gmail.com Imron imronnawawi@gmail.com <p><strong>A</strong><strong>BSTRACT</strong></p> <p>One of the main problem in giant river prawn (GFP) culture is early gonadal maturation in female resulting a reduced growth performance. This problem cause economic losses. When GFP at gonadal maturation, somatic growth will be inhibited because energy is used for reproduction. A factorial experimental design using two factors, namely dopamine and medroxyprogesterone, with each factor consist of three levels was applied. Three dopamine levels were 0, 10-5 mol/shrimp, and 10-10 mol/shrimp, while the medroxyprogesterone levels were 0, 75 mg/1.5 mL/bodyweight, and 150 mg/3 mL/bodyweight with a density 15 individual/tank. The utilization of dopamine and medroxyprogesterone in GFP (initial bodyweight : 11.27 ± 0.97 g) through injection at the third periopod was done three times at week 0, 2nd, and 4th with two weeks interval. The results showed that hormone inhibitor treatments affected both growth and reproductive performances in female GFP. The treated individuals showed a lower gonadal maturity indicator values and faster growth rate than control. Gonadal maturity, as shown by gonad histology, in all treatments were lower (previtelogenic and vitellogenic stages) than that in control which is in mature stage. Estradiol concentration premix dopamine 10-10 mol/shrimp and medroxyprogesterone 150 mg/3 mL/bodyweight treatments are lower than control. In conclusion, dopamine and medroxyprogesterone administration could suppres GSI and gonad development, and also increase growth rate.</p> <p>Keywords: Macrobrachium rosenbergii, dopamine, medroxyprogesterone, gonad development, growth.</p> 2020-02-18T07:26:15+07:00 Copyright (c) 2020 Jurnal Akuakultur Indonesia https://journal.ipb.ac.id/index.php/jai/article/view/30277 Eukaryote microbes potential for bioflocs in the swamp aquaculture 2020-04-09T05:39:50+07:00 Marini Wijayanti mariniwijayanti@fp.unsri.ac.id Tanbiyaskur qurhadi30@gmail.com Dade Jubaedah dadejubaedah@fp.unsri.ac.id Ade Bayu Saputra adebayusaputraa@gmail.com Karta Sari Genti kartasariganti11@gmail.com Agustina agustinasalim05@yahoo.co.id Nabila Saraswati saraswatinabil008@gmail.com Siti Yuliani sitiyuliani07@gmail.com Hary Widjajanti haryunsri@yahoo.com <p><strong>ABSTRACT</strong></p> <p><strong>&nbsp;</strong></p> <p>Eucaryote microbes have an important role in forming bioflocs in the brackishwater aquaculture ponds.&nbsp; Bioflocs become potential live feed for milkfish or crustacea. This study aimed to identify the potential of eukaryotic microbes in the brackishwater aquaculture as the biofloc candidates potential development. This study was done through the water quality assessment and potential indigenous microbes isolation approach. Sampling was retrieved from the water subcomposite and sediment on each intertidal inlet and outlet. The water quality of brackishwater pond and intertidal swamp tended to lack of nutrient as containing inoptimal dissolved oxygen, salinity, pH, and ammonia content for the milkfish culture. Therefore, liming and fertilizing should be done to enrich the indigenous microbes. Eucaryote microbe isolated from the enrichment media was the aquatic fungi (multicellular and unicellular fungi) and microalgae (bacillariophyta, chlorophyta, and cyanophyta). Fungi and yeast formed a floculation with microalgae. This form will become a biofloc candidate as a live feed and water quality controller for the development of brackishwater aquaculture in the swamp area.</p> <p>&nbsp;</p> <p>Keywords:&nbsp; eucaryote microbes, biofloc, aquaculture, pond, brackish water swamp</p> <p>&nbsp;</p> <p><strong>ABSTRAK</strong></p> <p><strong>&nbsp;</strong></p> <p>Mikrob eukariot berperan penting dalam pembentukan bioflok di tambak budidaya pada lahan rawa payau. &nbsp;Bioflok menjadi pakan alami untuk budidaya ikan bandeng atau udang. Tujuan riset ini adalah mengidentifikasi mikrob eukariot rawa payau potensial untuk pengembangan kandidat bioflok di akuakultur rawa payau.&nbsp; Riset ini dilakukan dengan pendekatan kualitas air serta isolasi mikrob <em>indigenous </em>tambak dan rawa pasang surut untuk mendapatkan isolat mikrob potensial sebagai kandidat pembentuk bioflok. Sampling dilakukan secara subkomposit pada air dan sedimen di setiap <em>inlet</em> dan <em>outlet</em> pasang surut. Kualitas air tambak dan rawa pasang surut menunjukkan kondisi oksigen terlarut, salinitas, pH, dan amonia berada di luar batas optimum budidaya ikan bandeng, sehingga perlu dilakukan pengapuran dan pemupukan untuk memperbanyak mikrob <em>indigenous-</em>nya. Isolasi mikrob eukariot dari media pengayaan diperoleh jenis fungi akuatik yang multiseluler dan uniseluler, sedangkan mikroalga yang diperoleh yaitu dari golongan Bacillariophyta, Chlorophyta, dan Cyanophyta. Fungi yang dapat membentuk hifa dapat merangkai mikroalga dan khamir untuk membentuk flok. Susunan ini diharapkan dapat menjadi bahan bioflok yang berguna sebagai pengendali kualitas air sekaligus pakan alami bagi pengembangan budidaya di lahan rawa payau.</p> <p>&nbsp;</p> <p>Kata kunci:&nbsp; mikrob eukariot, bioflok, budidaya, tambak, rawa payau</p> 2020-04-07T10:36:07+07:00 Copyright (c) 2020 Jurnal Akuakultur Indonesia https://journal.ipb.ac.id/index.php/jai/article/view/29645 Economic feasibility study of Litopenaeus vannamei shrimp farming: nanobubble investment in increasing harvest productivity 2020-04-15T01:25:28+07:00 Syifa Mauladani syifa@nano.or.id Asri Ifani Rahmawati fani@nano.or.id Muhammad Fahrurrozi Absirin fahrurrozi@nano.or.id Rizki Nugraha Saputra rizki@nano.or.id Aprian Fajar Pratama aprian@nano.or.id Arief Hidayatullah ariefh@nano.or.id Agus Dwiarto agus.dwiarto@nano.or.id Ahmad Syarif syarif@nano.or.id Hardi Junaedi hardi_j@apps.ipb.ac.id Dedi Cahyadi dedi@nano.or.id Henry Kasman Hadi Saputra henry_khs@apps.ipb.ac.id Wendy Tri Prabowo wen_aquaculture@yahoo.com Ujang Komarudin Asdani Kartamiharja ujang_k66@yahoo.com Alfian Noviyanto a.noviyanto@nano.or.id Nurul Taufiqu Rochman nurul@nano.or.id <p>This study aimed to evaluate the economic feasibility of <em>Litopenaeus vannamei</em> shrimp reared at 400 shrimp/m<sup>2</sup> in 56 days of culture. The experimental design was set in an 800 m<sup>2</sup> HDPE pond installed with nanobubble and non-nanobubble. Shrimp survival and total harvest in nanobubble treatment was increased to 92% and 2,255 kg, respectively. Economic parameters calculated in this study were Net Present Value (NPV), Internal Rate of Return (IRR), Payback Period (PP), Break Even Point (BEP), Benefit Cost Ratio (B/C Ratio), and Sensitivity Analysis (SA). The total investment required to run this farming practice is IDR 182,887,700. Total revenue per cycle is estimated at IDR 157,850,000 with the selling price of IDR 70,000/kg of shrimp. The estimated PP is 4 cycles, with an NPV of IDR 172,329,247 projected in 10 cycles. IRR is estimated at 18% and BEP is reached after 7,058 kg production of shrimp. B/C Ratio is estimated to be 1.26 and SA showed that productivity is the most affecting parameters in the present analysis. Based on the economic study, vannamei shrimp farming associated with nanobubble system is feasible to be realized.</p> 2020-04-15T01:25:27+07:00 Copyright (c) 2020 Jurnal Akuakultur Indonesia https://journal.ipb.ac.id/index.php/jai/article/view/30656 Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B. 2020-05-05T10:41:09+07:00 Akmal Izwar akmal.izwar@yahoo.co.id Sri Nuryati sri.nuryati606@gmail.com Rahman rahmanaqua@gmail.com Rini Purnomowati rinday_17@yahoo.co.id <p><strong>ABSTRACT</strong><br>This study aimed to obtain the pathogenicity of isolated bacteria from maribund barramundi <em>Lates calcarifer</em> with <br>black body syndrome (BBS). Moribund barramundi was collected from the Center for Mariculture Development <br>(BBPBL), Lampung Province. Five of pathogenic bacteria were found, i.e. <em>Pseudomonas stutzeri</em> L01, <em>Vibrio</em><br><em>harveyi</em>, <em>Bacillus cereus</em>, <em>Salinococcus roseus</em>, and <em>Pseudomonas stutzeri</em> L02. The bacteria were tested for LD50 <br>to obtain two types of high virulent bacteria to be used for the pathogenicity test. <em>P. stutzeri</em> L01 and <em>V. harveyi</em> were <br>the most virulent bacteria with a bacterial density of 107 CFU/mL. Both bacteria were used for pathogenicity test <br>with three treatments: injection of <em>P. stutzeri</em> L01, <em>V. harveyi</em>, mix <em>P. stutzeri</em> L01 and <em>V. harveyi</em>, and phosphatebuffered saline as control. Clinical symptoms showed a blackened body, bleeding under the belly, and inactive. <br>Mortality of fish injected with <em>P. stutzeri </em>L01, <em>V. harveyi </em>and mix <em>P. stutzeri</em> L01 and <em>V. harveyi</em> was 53.33%, <br>55.00%, and 58.33%, respectively. Erythrocyte and hemoglobin in all treatments were not significantly different <br>(P&gt;0.05). However, there was significantly different in the total leukocytes of mixed <em>P. stutzeri </em>and <em>V. harveyi</em><br>treatment (P&lt;0.05). In conclusion, <em>P. stutzeri</em> L01, <em>V. harveyi</em> and mix of them resulted in black body syndrome <br>(BBS) disease. Coinfection of <em>P. stutzeri</em> L01 and <em>V. harveyi</em> produce higher mortality than single infection.<br>Keywords: barramundi, black body syndrome, histopathology, pathogenicity<br><strong>ABSTRAK</strong><br>Penelitian ini bertujuan mendapatkan patogenisitas bakteri dari ikan kakap putih <em>L. calcarifer</em> yang memiliki <br>gejala <em>black body syndrome</em> (BBS). Ikan diambil dari Balai Besar Pengembangan Budidaya Laut (BBPBL), <br>Lampung. Lima jenis bakteri patogen ditemukan, yaitu <em>Pseudomonas stutzeri</em> L01, <em>Vibrio harveyi</em>, <em>Bacillus cereus</em>, <br><em>Salinococcus roseus</em>, dan <em>Pseudomonas stutzeri</em> L02. Bakteri diuji LD50 untuk mendapatkan dua jenis bakteri <br>virulen tinggi yang akan digunakan untuk uji patogenisitas. <em>P. stutzeri</em> L01 dan <em>V. harveyi</em> adalah bakteri paling <br>virulen dengan kepadatan bakteri 107 CFU/mL. Uji patogenisitas dengan tiga perlakuan: injeksi <em>P. stutzeri </em>L01, <br><em>V. harveyi</em>, campuran <em>P. stutzeri</em> L01 dan <em>V. harveyi</em>, serta <em>phosphate-buffered saline</em> sebagai kontrol. Gejala klinis <br>menunjukkan tubuh menghitam, perdarahan di bawah perut, dan pergerakan tidak aktif. Mortalitas ikan yang <br>disuntik dengan <em>P. stutzeri</em> L01, <em>V. harveyi </em>dan campuran <em>P. stutzeri</em> L01 dan <em>V. harveyi</em> masing-masing sebesar <br>53,33%, 55,00%, dan 58,33%. Eritrosit dan hemoglobin pada semua perlakuan tidak berbeda nyata (P&gt;0,05). <br>Namun, ada perbedaan yang signifikan dalam total leukosit perlakuan campuran <em>P. stutzeri</em> L01 dan <em>V. harveyi</em><br>(P&lt;0,05). <em>P. stutzeri</em> L01, <em>V. harveyi</em> dan campuran <em>P. stutzeri</em> L01, <em>V. harveyi </em>dapat menyebabkan <em>black body</em><br><em>syndrome</em> (BBS). Koinfeksi <em>P. stutzeri </em>L01 dan <em>V. harveyi </em>menghasilkan mortalitas lebih tinggi daripada infeksi <br>tunggal.<br>Kata kunci: barramundi, <em>black body syndrome</em>, histopatologi, patogenisitas</p> 2020-05-05T10:41:08+07:00 Copyright (c) 2020 Jurnal Akuakultur Indonesia