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Two random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (Creb-2) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (Creb-2) in melon. SCOPE14 derived from OPE-14 yielded a single DNA band at 541 bp, while SCAPB05 derived from APB-05, yielded a single DNA band at 1,046 bp, respectively. Segregation of SCOPE14 and SCAPB05 markers in bulk of F2 plants demonstrated that they were co-segregated with RAPD markers from which the SCAR markers were originated. Furthermore, results of SCAR analysis in diverse melons showed SCAPB05 primers obtained a single 1,046 bp linked to Creb-2 in resistant cultivars Sanuki-shirouri and Kohimeuri. However, SCOPE14 failed to detect Creb-2 in diverse melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to CMV-B2 by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.
Key words: Cucumis melo L., Creb-2, RAPD, SCAR
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HAYATI Journal of Biosciences (p-ISSN: 1978-3019; e-ISSN: 2086-4094) Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University (Bogor Agricultural University), Dramaga Campus, Bogor 16680, Indonesia Phone/Fax: +62-251-8421258; E-mail: hayati.jbiosci@apps.ip.ac.id; URL : http://journal.ipb.ac.id/index.php/hayati |
This journal is published under the terms of the https://creativecommons.org/licenses/by-nc/4.0/ |