HAYATI Journal of Biosciences

The Potency of Centella asiatica Leaf Extract on VEGF Expression and Angiogenesis in Second-Degree Burn Wound in Mice

Frederick Surya Utoyo, Wahyu Widowati, Hana Ratnawati — Wed, 13 Nov 2024 10:04:09 +0700

Burn injuries present a significant global health challenge, with the highest incidence rates reported in Southeast Asia, including Indonesia. Healing burn wounds is a complex and dynamic process involving various cellular and molecular mechanisms, prominently featuring the role of Vascular Endothelial Growth Factor (VEGF) in tissue regeneration and wound repair. VEGF is crucial for inducing and regulating angiogenesis and supplying oxygen and nutrients to the healing tissue. This study aims to evaluate the potential of pegagan (Centella asiatica) leaf extract cream 1%, 3%, and 5% daily for 14 days in enhancing VEGF expression and angiogenesis in second-degree burn wounds in mice (Mus musculus). This study investigates the application of C. asiatica extract cream on second-degree burn wounds in mice, comparing its effects on VEGF protein expression and angiogenesis to those of base cream and silver sulfadiazine cream, with outcomes evaluated using immunohistochemistry (IHC) and hematoxylin and eosin (HE) staining methods. Our findings suggest that C. asiatica extract cream promotes reduced burn wound size, significant upregulated VEGF expression, and enhanced angiogenesis in treating burn wounds compared to positive control, with a 5% dose having the best result. The study concludes that C. asiatica extract cream may effectively treat burn wound healing through enhancing VEGF expression and angiogenesis.

The Effect of Matriconditioning Enriched Biofertilizer and Washed Rice Water to Enhance Seed Germination, IAA Content and Seeding Growth on Shallot (Allium cepa L.)

Sundahri, Muhamad Wahyu Saputra — Mon, 23 Sep 2024 09:02:26 +0700

Shallot seeds are a healthier option for cultivating shallots, as they reduce seed-borne disease infections, but they have a low germination rate. This study examined the impact of matriconditioning enriched with plant growth-promoting rhizobacteria (PGPRs) and rice washing water (WRW) on increasing germination, IAA content and growth of shallot seeds. The matriconditioning treatments included P0 (control), P1 (PGPR-absent), and PGPR presence in P2 (PGPR-Rhizomax), P3 (PGPR-BenprimA), and P4 (PGPR-FloraOne). The WRW treatments included L0 (0%), L1 (50%), and L2 (100%). The shallot seeds used are deteriorated, with moisture content and germination rates below the quality standards set by the Ministry of Agriculture of the Republic of Indonesia. The research revealed that treatment with P2 was the best result on seed germination and seedling root length. The treatment with P4 was the best result on seedling dry weight. The WRW treatment with L2 exhibited the best result in seed germination, seedling root length, and seedling dry weight. The combination of P2L2 demonstrated the best result on seed germination. The treatment with matricondiitoning increased shallot sprouts IAA content in 13-day-olds compared to controls. Also, the treatment with WRW linearly increased the IAA content in 13-day-old shallot sprouts.

Evaluation of Antibacterial and Antibiofilm Effects from Soil Streptomyces spp. against Multidrug-Resistant Bacteria

Mon, 07 Oct 2024 00:00:00 +0700

The global increase in multidrug-resistant (MDR) bacterial infection has rapidly gained concern globally. This study aimed to investigate antibacterial and antibiofilm potential of 25 soil actinomycete strains against MDR strains including Escherichia coli strain M4, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M18, and Methicillin Resistant Staphylococcus aureus (MRSA). In this study, three actinomycete isolates encoded APM-7, APM-11, and APM-21 showed a strong and broad antibacterial spectrum. The minimum inhibitory concentration (MIC) of extracts derived from these isolates was ranged from 78 μg/ml to 10,000 μg/ml. In addition, The extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract had the best antibacterial and antibiofilm activities with the strongest values against MRSA. According to the nucleotide sequencing of the 16S rRNA gene, APM-7, APM-11, and APM-21 strains possessed similar identities with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds, namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contained two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities. Moreover, new insights were provided into a promising candidate for use in an active compound combating strategy to control MDR bacterial strain infection.

Evaluation of Phenotypic and Genetic Characteristics of Dryobalanops aromatica (Dipterocarpaceae) Seedlings Growing in Peat and Mineral Media

Tue, 08 Oct 2024 00:00:00 +0700

The selection of tree species suitable for restoration in diverse site conditions is still a challenge. Therefore, this study aimed to analyze the phenotypic and genetic characteristics of Dryobalanops aromatica seedlings growing in mineral soil media and peat media. Phenotypic characteristics were evaluated by analyzing the growth performance, leaf morphological characteristics, leaf color, and chlorophyll content, while genetic characteristics were evaluated by using microsatellite markers. The study revealed that seedlings planted in peat media exhibited greater height and shoot length growth, although the difference was not statistically significant compared to those in mineral soil media. However, seedlings in mineral soil media displayed a significantly higher survival rate than those in peat media. Peat-grown seedlings had higher chlorophyll content in their leaves, while mineral soil-grown seedlings displayed a lighter green leaf color based on digital analysis. Genetic analysis indicated a high genetic diversity of overall D. aromatica seedlings (He = 0.635) and unclear genetic structure between the two media. Overall, these findings demonstrate the successful adaptation of D. aromatica seedlings to peat media, positioning them as a promising choice for peatland restoration.

Evaluation of Hursitism for Polycystic Ovary Syndrome Women At Al- Ramadi City

Mon, 11 Nov 2024 10:39:45 +0700

The hyper-androgen and huritism in face and body in women is important diseases. It can be used as a possible marker in patients with ovary polycystic syndrome (PCOS). This study was carried out to evaluate DHEA and Testosterone levels associated with huritism and PCOS was studied in serum samples from pregnant, and non-pregnant patients and controls attending clinic Specialized. The study included 38 samples divided into three groups: 21 patients subjects, 6 pregnant women, 15 non-pregnant women with PCOS syndrome, and 17 normal subjects as control of the same age group. The target was selecting pregnant women due to changes in hormonal levels and physiological changes, as well as comparing them with non-pregnant women and control samples. The level of DHEAS was considerably higher in the median among pregnant women than in the control group and between non-pregnant and control groups without statistically significant differences between pregnant and non-pregnant values. Similarly, testosterone shows a statistically significant higher median among pregnant than control and also between non-pregnant and control groups without statistically significant difference between pregnant and non-pregnant values. In conclusion, this study detected a significant increase in testosterone and DHEA in non-pregnant women and then followed by pregnant women compared to the control, and age and the rise of these hormones are factors that cause the appearance of thick hair and thus cause polycystic ovary, which may lead to infertility.

Characterisation of ACE2-like Enzyme from Bacillus cereus sp. as an Alternative Treatment for COVID-19 Patients

Alya Sendra Maulida, Novi Arie Anggraeni, Azzania Fibriani — Mon, 11 Nov 2024 10:55:32 +0700

SARS-CoV-2 infection can lead to downregulation of ACE2, raising pro-inflammatory Angiotensin II levels. Recombinant human ACE2 protein therapy can restore homeostasis but is costly. An alternative is an ACE2-like enzyme with similar effects. The previous research identified a carboxypeptidase protein from Bacillus cereus sp. (rBceCP) as a potential ACE2-like enzyme, but it has not been characterized well. This study characterizes, expresses, and tests the activity of the rBceCP). rBceCP structure and properties were predicted using Prabi and Expasy. The in vitro approach included protein expression optimization, hydrolysis tests, and inhibition tests. In silico analysis revealed the protein is a homodimer with 53.66% α-helix and a molecular weight of 58.99 kDa. The protein is stable, hydrophilic, and has an isoelectric point at pH 4.93, indicating it can be expressed using the E. coli system. Expression of rBceCP showed no significant differences across IPTG concentrations (p-value >0.05). The protein hydrolysis activity of rBceCP was similar to the control, though purified samples had lower activity than crude samples. Inhibition activity in crude and purified samples showed no significant differences (p-value >0.05) and was higher than the control. Thus, rBceCP has potential as an ACE2-like enzyme and a therapeutic candidate for COVID-19.

Characterization of Antibacterial Compounds from Marine Sponge-associated Streptomyces spp. against Some Pathogenic Bacteria

Firda Sri Efendi, Sri Budiarti, Yulin Lestari — Mon, 11 Nov 2024 11:27:08 +0700

The increasing trend of antibiotic resistance among pathogenic bacteria is a worldwide problem. Streptomyces produce a number of bioactive compounds such as antibacterial. This study aimed to investigate the effect of different media and incubation time in increasing the antibacterial activity of marine sponge-associated Streptomyces spp. and characterize antibacterial compounds of marine sponge-associated Streptomyces spp. against pathogenic bacteria. Among the three tested media and some days of incubation times, Streptomyces spp. produce more antibacterial activity when grown using modified molasses medium at 15 days incubation. The ethyl acetate extracts of Dbi28t exhibited a significant inhibitory zone against Extended Spectrum β-Lactamase (ESBL)-producing Escherichia coli, Providencia rettgeri then followed by Proteus mirabilis and Pseudomonas putida and the results were higher than some commercial antibiotics. This study has identified nine antibacterial compounds in Dbi28t using Liquid Chromatography-tandem Mass Spectrometric (LC-MS/MS) analysis, with the most abundance belonging to pumilacidin A, then followed by pumilacidin B, surfactin B, surfactin A, phenazostatin B, chalcomycin B, neopyrrolomycin C, saquayamycin A and saphenamycin. This work provides the first report from a Streptomyces sp. Dbi28t produced pumilacidin, surfactin and other bioactive compounds with the modified molasses medium for optimization of characterization of its antibacterial compounds.

The Effectiveness of Insectary Plant that Attracts and Sustains Beneficial Arthropods to Control Spodoptera frugiperda J.E Smith (Lepidoptera: Noctuidae) in Maize

Vien Sartika Dewi, Sylvia Sjam, Sulaeha Sulaeha, Elsa Sulastri — Mon, 11 Nov 2024 12:03:27 +0700

Spodoptera frugiperda J.E. Smith, or fall armyworm (FAW), is a significant pest in maize plants and can reduce crop yields. Synthetic insecticides are still used to control S. frugiperda, but their use harms the environment and non-target organisms and can cause pests to become resistant. For this reason, alternative environmentally friendly technologies are needed, such as habitat management by planting insectary plants. This research aims to evaluate the potential of insectary plants to attract beneficial arthropods to suppress the S. frugiperda population and minimize the damage caused. This research activity was carried out using field research. The treatment in this research was the type of insectary plant used, and as a comparison, observations were made on land managed by farmers. The research results show that habitat management by planting insectary plants can increase the population of beneficial arthropods such as Coccinellidae, Formicidae, Miridae, Staphylinidae, and Araneidae. Increasing the population of beneficial arthropods can suppress the population of S. frugiperda so that its damage decreases and yields increase. These results show that planting insectary plants has the potential to prevent outbreaks of S. frugiperda, which can be combined with the application of other environmentally friendly technologies.

Dynamic Changes of Gut Microbiome in Borneo Earless Monitor Lizard Across Different Diets

Milsa Solva Diana, Ainissya Fitri, Meryandini Anja, Roni Ridwan — Mon, 11 Nov 2024 12:56:02 +0700

The Borneo earless monitor lizard (Lanthanotus borneensis) is one of the endemic animals of Indonesia. In 2019, the IUCN listed L. borneensis as an ‘endangered’ species. The declining population of the L. borneensis is a major concern for conservation efforts. One of the efforts to conserve endangered wild animals is to know the gut microbiome profile and the relationship between the host and the bacterial community in the body, which is associated with an influence on health. This research aims to determine the gut microbiome profile of animals with metagenomic analysis using Next-Generation Sequencing (NGS). This research was carried out by providing three feed types: earthworms, shrimp, and fish. Sample feces was collected by cloacal swabs and the DNA was sequenced using the Illumina NGS technique in the V4 16S rRNA region to examine the gut microbiome. The results of the metagenomic analysis showed differences in bacterial abundance in each feed treatment. The L. borneensis treated with earthworms and fish were dominated at the phylum level by Proteobacteria and Bacteriodota, while in shrimp, the phyla were Proteobacteria and Firmicutes. Index Shanon, Simpson, and the rarefaction curve also showed the highest bacterial diversity found in the fish treatment. This study can be used as a reference in designing suitable feed formulations for effective captive breeding for the conservation of L. borneensis

Distribution and Utilization of Musa spp. by Nocte Tribe in Tirap District, Arunachal Pradesh, India

Walung Songthing, Govinda Pangging, S. Sureshkumar Singh — Mon, 11 Nov 2024 13:58:15 +0700

The present study emphasized the distribution and utilization of both wild and domesticated Musa spp. of the Nocte tribe from Tirap district, Arunachal Pradesh. An extensive field survey was conducted from July to October 2023. A total of 62 households were surveyed randomly with the help of a semi-constructed questionnaire and personal interview. About 69% of the informants were from the age group of 31-60 years. About 84% live in a nuclear family type. Maximum informants were male (74.19%) and were married (80.64%). Only 17.75% of the informants were illiterate and were farmers (46.77%). In this study, 13 wild Musa spp. were reported from Tirap district, and 4 Musa spp. were found to be cultivated by the tribe. Of these, 76.47% were wild, and the rest were domesticated. M. itinerens had a widespread distribution range and occurred in various habitats between 155 and 1,711 masl altitudes. The highest use percentage was found in the Edible use category with 33%, followed by Ceremonies and Rituals (19%), Commercial uses (12%), and both Other and Packing purposes (10% each). Among the plant parts, inflorescences had the highest usage with 30%, followed by leaf (24%), pseudo stem (21%), etc. Among all Musa spp., M. itinerens had the highest number of usages (14 uses), followed by M. nagensium (12 uses).

Effect of Synthetic Fertilizer on Diazotrophic Bacteria in Secondary Forest and Oil Palm Soils in Central Kalimantan

Mon, 11 Nov 2024 14:19:20 +0700

Soil microorganisms, especially diazotrophic bacteria, are vital for ecosystem sustainability, significantly contributing to nitrogen cycling and biodiversity conservation. Understanding the impact of different land-use practices on soil microorganisms, especially synthetic fertilizer addition, is essential for sustainable agriculture. This study compares soil properties, bacterial densities, and responses to fertilization in secondary forest soils and adjacent oil palm plantation soils located in PT Kerry Sawit Indonesia, Central Kalimantan. A greenhouse experiment was conducted using both secondary forest and oil palm plantation soils to evaluate the impacts of different synthetic fertilizers on nitrogen-fixing bacteria and soybean agronomic performance. Total soil bacteria and diazotrophic bacteria, essential for nitrogen fixation, were analyzed through microbiological assays and qPCR focusing on the nifH gene. Our findings show that oil palm plantation soils had higher levels of nitrogen, phosphate, and nitrate, while secondary forest soils had a higher abundance of diazotrophic bacteria. Furthermore, excessive nitrogen fertilization was found to reduce microbial density, negatively impacting plant sustainability, highlighting the importance of customized fertilizer management. The study highlights the intricate connections between land-use practices and microbial populations, providing valuable insights for balancing agricultural productivity with ecological sustainability.

Evaluation of Drought Tolerance Ability in Wheat Genotypes Through Comprehensive Stress Indices

Wed, 13 Nov 2024 08:43:45 +0700

The objective was to assess a range of stress indices to discern wheat genotypes resilient to drought stress, so forty-nine genotypes underwent scrutiny in both drought stress in rainfed conditions and non-stress settings (with supplementary irrigation), employing a 7 × 7 lattice layout with two replicates across years 2019 and 2020. The evaluation incorporated twenty stress indices anchored in yield under water stress (YS) and potential (YP) circumstances. Primary analysis indicated that eight indices (RDI, YSI, YI, K₂STI, MRP, REI, RR and SSPI) did not give any new information, so they were eliminated in further analysis. Genotypes G33 (4234 kg ha^-1) and G9 (2227 kg ha^-1) were the best genotypes based on YP in 2019 and 2020, respectively. A positive association was observed between ATI and YP and between YS with DI and K1STI in the year 2019, while in the second year, such positive associations were not seen. We found some wheat genotypes G6, G9, G10 and G11 demonstrated high performance in both potential and rainfed conditions across two years, showing yield higher than 1,800 and 2,700 kg ha^-1 for YS and YP, respectively, across both years. These genotypes were detected as the most tolerant genotypes by mean-based indices (TOL, HM, GMP, and MP) as well as SSI and ATI indices, so it can be concluded that these indices are more useful than other indices for identifying the most tolerant as well as the high yielding genotypes.

Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.)

Mahat Magandhi, Sobir, Deden Derajat Matra, Yudiwanti Wahyu, Sudarmono — Wed, 13 Nov 2024 09:20:51 +0700

Durio testudinarius, known as Durian Kura-Kura, is a native Bornean durian species in the Malvaceae family with fruit at the base of the main stem. Recent advances in genomic-based next-generation sequencing are being used to conserve germplasm and enhance plant breeding. This method rapidly and cost-effectively sequences plant genomes, allowing for simple sequence repeat markers to be constructed in silico. This study aimed to design and evaluate novel, simple sequence repeat markers utilizing next-generation sequencing microsatellite data of D. testudinarius. A total of 20 sequences containing di-, tri-, tetra-, and penta-nucleotide motif repeats were selected and designed to generate primers using Primer3 online software. The PCR results of the twenty primers tested on D. testudinarius and 19 genomic DNA samples of species from the Malvales order showed successful amplification. These newly developed simple sequence repeat markers are crucial for genetic population analyses, determining population structure, and enhancing plant breeding programs and conservation strategies for D. testudinarius and its relative's germplasm resources.

Optimization of Genetic Material Extraction Techniques and Application of Isothermal Amplification Method for Field Authentication of Two Thresher Sharks (Alopias pelagicus and Alopias superciliosus)

Wed, 13 Nov 2024 10:16:00 +0700

The pelagic thresher shark (Alopias pelagicus) and bigeye thresher shark (Alopias superciliosus) are important shark species for Indonesia's consumption and finning industry. Both Alopias species are included in the CITES appendix II, thus requiring certain documents for trading. Regarding species identification for on-site application, the DNA isolation method is a crucial step. In this study, we developed a DNA isolation method suitable for on-site application based on isothermal amplification (LAMP) and species-specific COI gene markers. Three different extraction methods were applied, namely modified spin column kits and dipsticks. The quality of DNA was evaluated and tested for isothermal amplification using a reference sample, fresh fillet, and ethanol-preserved sample. The extracted sample concentration was in the range of 135.35-0.65 ng/μL. The LAMP test showed that three different DNA extraction methods successfully amplified the DNA fragments through the color changes at the end point of the LAMP reaction. The LAMP test was also sufficient to detect less than 10 ng of DNA from A. pelagicus and A. superciliosus within 30-50 min. The DNA from the modified spin column and dipstick extraction method combined with LAMP can potentially be used to detect Alopias pelagicus and Alopias superciliosus species on-site.

Cloning and Optimized Expression of Bst DNA Polymerase from Geobacillus stearothermophillus in Escherichia coli BL21

Intan Taufik, Rizal Fanany, Agika Manjaswari, Fenryco Pratama — Thu, 14 Nov 2024 11:06:01 +0700

Bst DNA polymerase possesses strand displacement activity, enabling isothermal DNA amplification without requiring a thermal cycler. This enzyme is utilized in the Loop-Mediated Isothermal Amplification (LAMP) method, which offers advantages in speed and simplicity over Polymerase Chain Reaction (PCR) method. The growing demand for Bst DNA polymerase highlights the need for cost-effective in-house production, as a commercial option is economically challenging. For that purpose, this study aims to construct and optimize the expression of Bst DNA polymerase from Geobacillus stearothermophilus in Escherichia coli. The expression constructs pET16b.BstHF vector was constructed using Gibson Assembly and expressed in E. coli BL21 (DE3). Optimal expression was achieved with 1 mM IPTG, induction at OD₆₀₀ 0.8 and 6-hour induction time. The purified enzyme was achieved with a protein yield of 2,175 mg/L culture and demonstrated effective polymerase activity for LAMP.

Fermentation and Microencapsulation of Red Palm Oil as a Nutraceutical Source

Thu, 14 Nov 2024 15:48:24 +0700

Red palm oil (RPO) has various bioactive and nutritional components with high potential to be developed as a source of nutraceuticals in addition to its potential as a functional food. The fermentation technology is a processing process that affects metabolite activity, increasing the durability of a product. Staphylococcus epidermidis is one of the bacteria that is generally used in the sugar and oil fermentation process. RPO fermentation by Staphylococcus epidermidis is supposed to increase nutraceutical value by adding probiotic properties to RPO products. Moreover, the instability of bioactive compounds in RPO needs to be protected with coating technology microencapsulation. So, this study aims to formulate a microencapsulation procedure for fermented RPO using Staphylococcus epidermidis ATCC 12228 as a potential nutraceutical. RPO was fermented using S. epidermidis with three variations of MRS media and RPO comparison, then microencapsulated using emulsification and extrusion methods. The physicochemical properties and bioactivity of the product, microcapsule, were then analyzed. Our data shows that RPO fermentation was able to increase the chemical components. RPO fermentation produced more 1,2-Benzenedicarboxylic acid (2-Ethylhexyl) ester. Furthermore, microencapsulation of fermented RPO has better yield, efficiency, and solubility in water than non-fermented RPO microencapsulation. Moreover, our work also shows that the microencapsulation process increased RPO stability.

Small But Healthy: An Adaptive Response in Baduy Children

Fri, 15 Nov 2024 09:06:05 +0700

Life history explains natural selection, resulting in phenotypic plasticity that can be studied through the growth pattern (growth rate and body size). The Baduy People, one of the indigenous peoples in Indonesia, exhibit prolonged growth, a slow growth rate, a low peak growth spurt, and small adult size as an adaptation to challenging biocultural conditions. The life history trade-offs of the Baduy People are demonstrated by ontogenetic allometry, which synchronizes between skeletal growth and future reproduction. The study aims to characterize the growth of Baduy children aged 0-5 years with more precise ‘tracking’ of developmental plasticity. We collected anthropometric data (body height-BH, body weight-BW, head circumference-HC, body mass index-BMI) using a cross-sectional method from 118 girls and 97 boys aged 0-5 years across 24 Baduy Luar hamlets. Data were analyzed with Generalized Additive Models for Location, Scale, and Shape (GAMLSS) in R version 4.2.1. Nutritional statuses were categorized based on the BMI z-score. The growth pattern showed a decline in growth rate after birth until 2 or 3 years, but the body size increased yearly. The body size (BH and BW) of Baduy children is lower than the Indonesian National Synthetic Growth Charts (INSGC), but most of them have a good nutritional status (>80%). This finding is consistent with the "small but healthy" hypothesis: a small body is an advantageous evolutionary strategy for energy efficiency and maximizing growth potential in challenging biocultural conditions.

Genetic Characterization of Sumatran Mirah Chicken Based on Mitochondrial D-loop Region Sequence

Parsaoran Silalahi, Tunggul Ferry Sitorus, Widya Pintaka Bayu Putra — Fri, 15 Nov 2024 10:30:40 +0700

Mirah chickens are Indonesian indigenous chickens that originate from Simalungun Regency, North Sumatera Province of Indonesia. The study aimed to determine the genetic characterization of Mirah chicken based on the mitochondrial D-loop region (838 bp). Twenty Mirah cocks from Simalungun Regency of Indonesia were used in the present study. The results showed that fourteen haplotypes were found in the studies of birds based on sixteen mutation sites. Therefore, these haplotype and nucleotide diversities in the partial D-loop region of the Mirah chicken were classified as high. The Median-joining tree revealed that the Mirah chickens were classified in a similar cluster with the Red jungle fowl (Gallus gallus). Ten haplotypes of birds were close to G. g. bankiva, and four haplotypes of birds were close to G. g. gallus. In conclusion, Mirah chickens had the genetic introgression from two sub-species of Red junglefowl, i.e., G.g. gallus and G.g. bankiva. Hence, the pure breeding program for Mirah chickens is important to conserve their genetic resources from extinction.

Evaluating the Cytotoxic Effects of Ethanol and n-Hexane Extracts from Black Cumin Seeds (Nigella sativa) on B16F10 Mouse Melanoma Cells : A Preliminary Investigation into Vitiligo Treatment

Dian Pratiwi, Silmi Mariya, Raendi Rayendra, Agus Setiyono — Fri, 15 Nov 2024 13:21:51 +0700

Black cumin (Nigella sativa) is a herbal plant that has been cultivated locally in Indonesia and is traditionally used for various diseases. Thymoquinone, one of the main components, is rich in biological activity. In several countries, topical application of its oil on human skin with vitiligo can stimulate skin repigmentation with minimal side effects. This study aims to determine the viability of B16F10 melanoma mouse cells against ethanol and n-hexane extracts of black cumin seeds through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ethanol extract (EE) yield was 14.306%, and the n-hexane extract (NHE) was 7.442%. Phytochemical screening of EE detected flavonoids, alkaloids, saponins, and steroids, and High-performance liquid chromatography (HPLC) detected 0.040% thymoquinone. The MTT test showed cell viability was >100% from EE at all treatment concentrations, namely 0.75-100 ppm and only 0.75-6.25 ppm for NHE. In conclusion, this study indicates that 96% EE of Nigella sativa is less toxic than NHE on B16F10 mouse melanoma cells and has potential as an alternative treatment for vitiligo, which needs to be proven in further research.

Diversity of Rhizospheric Bacterial Community from Kaolin Mining Site and Their Potential as Plant Growth Promoting Bacteria

Khafifah Armanisa, Iman Rusmana, Rika Indri Astuti — Wed, 20 Nov 2024 08:17:21 +0700

Tailing from mining activities affects soil fertility resulting in poor soil conditions that are challenging for plants to grow. Plants can interact with rhizosphere bacteria to enhance their growth in harsh environments. Rhizospheric bacteria possess numerous mechanisms that promote plant growth and induced resistance to various abiotic stress. This study aims to determine the diversity of rhizobacteria and their potential as plant growth-promoting rhizobacteria (PGPR) agents. Bacterial communities from rhizosphere soil samples from kaolin mining sites in Perawas, Tanjung Pandan district, Belitung Regency, Bangka Belitung Island, Indonesia were analyzed using Next Generation Sequencing based on the V3-V4 region of the 16S rRNA gene, while culturable bacteria were isolated from samples and screened for PGP activity. The results showed that the rhizosphere bacterial community was mostly dominated by Pseudomonadota, Acidobacteria, and Verrumicrobiota. There were 15 bacteria isolated from the sample and RKB-5 bacterial isolate had the potential to be PGP agent. The RKB-5 bacterial isolate was identified as Burkholderia cenocepacia based on its 16S rRNA sequence. The bacterial isolate produced IAA, utilized ACC, dissolved phosphate up to 209,5 mg/L, and formed a high potassium solubilizer index value of 5.00. Therefore, the B. cenocepacia RKB-5 has potential application as the PGPR to support plants growth by obtaining nutrients in ex-mining lands with poor soil conditions.

Analysis of Drought Stress Effect on Inpari Germination: Survival Method

Riza Yuli Rusdiana, Halimatus Sa'diyah, Alfian Futuhul Hadi — Wed, 20 Nov 2024 08:54:02 +0700

Drought stress using mannitol can inhibit the germination of rice variety seeds. These studies typically produce time-to-event data and censored observation. Survival analysis techniques are valuable for accounting for these non-germination events, as they describe how germination probability changes over time based on the likelihood of seed development. Until now, there have not been survival studies regarding rice germination affected by drought stress in Indonesia. Thus, we investigated the germination probability of three rice varieties (Inpari 19, Inpari 32, and Inpari 49) under drought stress using survival analysis. The seeds were germinated in 0%, 2%, 4%, 6%, and 8% concentrations of mannitol and evaluated daily over 14 days. Our results demonstrated that higher mannitol concentrations significantly decreased the germination percentage and delayed germination time. The survival rates varied significantly between different mannitol concentrations, highlighting the adverse effects of drought stress. However, there was no significant difference in the probability of seed germination among the varieties treated with 2% mannitol. Among the varieties studied, Inpari 19 is more likely to be drought-resistant compared to Inpari 32 and Inpari 49. It is based on the highest germination percentage, shortest germination time, and highest probability of germination compared.

The Survival, Growth, and Accelerating Morphological Development of Stichopus horrens are Affected by the Initial Larval Stocking Densities

Thu, 21 Nov 2024 11:08:25 +0700

Stichopus horrens is highly exploited due to their use as a pharmaceutical ingredient. Since then, this species has become extinct; therefore, it is necessary to start aquaculture. Gonad maturation and broodstock spawning succeeded, but the optimum larval-rearing stocking density has not yet been determined. Therefore, the present study aimed to determine the optimal stocking density to improve survival and accelerate the development of larval morphology. Three treatments were used: 100, 150, and 200 larvae L^-1, each with four replicates. Twelve 100-litre plastic containers filled with 80 litres of seawater as larval rearing media were placed in a concrete tank with a water-bath system using a heater and a thermostat (29.0°C±1.0°C). The larvae were fed a mixture of microalgae (Chaetoceros muelleri, Isochrysis galbana, and Nitzchia sp.) twice a day^-1. The data collected included survival and growth rates, larval morphological development, and water quality. The fastest metamorphose from auricularia to the doliolaria stage occurred in 100 larvae L^-1, 15 days after hatching. The highest survival rate, growth rate, and percentage of larvae metamorphose to the doliolaria stage were obtained in the 100 larvae L^-1 as 26.38%, 26.4 µm day^-1, and 65.27%, respectively, and were significantly different (P<0.05). A stocking density of 100 larvae L^-1 was optimal for promoting survival and growth and accelerating the morphological development of auricularia to the doliolaria stage.

Endophytic Actinomycetes of Liliaceae Plants as Biocontrol Agents of Fusarium oxysporum f.sp. cepae Causes of Basal Plate Rot Disease on Shallots

Lisa Marianah, Abdjad Asih Nawangsih, Abdul Munif, Giyanto, Efi Toding Tondok — Fri, 22 Nov 2024 09:48:02 +0700

Basal plate rot disease is one of the critical diseases in shallot plants. Control using synthetic chemical fungicides is still unable to overcome this problem, and it hurts the environment and reduces the population of essential microbes in plants. Endophytic actinomycetes have potential as biocontrol agents. They are reported to be able to inhibit the growth of pathogens, induce plant resistance, produce cell wall degrading enzymes, and promote growth. This research aims to obtain endophytic actinomycete isolates that have the potential to inhibit the growth of the fungus Fusarium. oxysporum f.sp. cepae causes of basal plate rot disease in shallot, and evaluate its inhibitory mechanism. Endophytic actinomycetes were isolated from tubers and roots of Liliaceae plants collected from shallots-production center area. The isolate obtained was tested for biosafety and continued with its inhibitory effectiveness against the fungus F. oxysporum f.sp. cepae in vitro, ability to induce resistance, and plant growth promotion test. The six best isolates were selected based on weighting using AHP and identified molecularly. The endophytic actinomycetes of Liliaceae plants can inhibit the growth of F. oxysporum f.sp. cepae up to 63.49% with an antibiosis mechanism, producing chitinase enzymes that cause lysis, induce resistance, and produce growth hormones such as IAA. Streptomyces sp. can inhibit the fungus F. oxysporum f.sp. cepae causes basal plate rot disease on shallot by producing antifungal compounds and chitinase enzymes, inducing resistance, and producing growth hormone.

Oleuropein Protects against the Development of Kidneys Induced by Paracetamol in Albino Male Rats.

Fri, 22 Nov 2024 13:54:53 +0700

Paracetamol treatment is considered one of the treatments used to relieve pain and antipyretic. Therefore, excessive doses and long-term use lead to organ toxicity. Paracetamol treatment is considered one of the treatments used to relieve pain and antipyretic. Therefore, excessive doses and long-term use lead to organ toxicity. The aim of the study was to investigate the protective effect of Oleuropein extracted from olive leaves on the physiological and histological aspects induced by Paracetamol in a rat model. The methods used 25 albino Swiss rats randomly distributed into five groups with the same number. The unit of control is given normal saline. Paracetamol (750 mg/kg) was injected into the group once. In the treatment groups (50 mg/kg, 100 mg/kg, 150 mg/kg). The Administration of Paracetamol's result significantly increased blood urea, creatinine, sodium, and potassium levels, and their blood concentrations decreased with Oleuropein (P 0.05). In addition, Oleuropein extracted from olive leaves relieved some symptoms, including acute vascular congestion caused by a dose of Paracetamol. Compared with paracetamol treatment, there is an infiltration of inflammatory cells and severe nephrotoxicity in the tubules. According to this study, the Oleuropein extracted from olive leaves can be used to prevent kidney damage, and It is not recommended to give Paracetamol, which increases kidney disorders.

Assessment of Genetic Diversity Using Morphological and Molecular Characteristics of Indonesian Zoysiagrass Genotypes

Rahayu, Fatimah, Yang Geun Mo, Eun Ji Bae — Mon, 25 Nov 2024 09:39:18 +0700

Zoysiagrass is warm-season turfgrass thriving in tropical regions. Despite the adaptive nature, the existence of Indonesian zoysiagrass as well as morphological and genetic characteristics are not available. Therefore, this study aimed to explore Indonesian zoysiagrass from western and eastern parts of the country, as well as its morphological and molecular characteristics. Morphological characteristics was conducted to measure vegetative and reproductive characters while genotyping was performed using 15 simple sequence repeat markers. Morphological characteristics cluster three major groups, namely Group 1 corresponded to short, shorter, and fine leaves. Group 2 corresponded to tall, longer, and fine leaves, fewer seeds, and short spikelet, while Group 3 corresponded to tall, long, and wider leaves, more seeds, and longer spikelet. The results showed that the expected heterozygosity (He = 0.256) was lower than homozygosity (Ho = 0.341). The high level of discriminating capacity, polymorphism, and informativeness of SSR marker was observed (Effective Multiplex Ratio = 4.20, Marker Index = 2.394, and Resolving Power = 1.574). Additionally, population structure generated two subpopulations. Group 1 corresponded to Z. japonica from Sumatera Island and mixed province while Group 2 corresponded to Z. japonica from Central Java and Bali with Z. japonica and Z. matrella from mixed province. In conclusion, the exploration of morphological and genetic diversity from Indonesian zoysiagrass provided useful insight for conservation and future breeding improvement.