DARYONO BS, WAKUI K, NATSUAKIK T. Two random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (Creb-2) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (Creb-2) in melon. SCOPE14 derived from OPE-14 yielded a single DNA band at 541 bp, while SCAPB05 derived from APB-05, yielded a single DNA band at 1,046 bp, respectively. Segregation of SCOPE14 and SCAPB05 markers in bulk of F2 plants demonstrated that they were co-segregated with RAPD markers from which the SCAR markers were originated. Furthermore, results of SCAR analysis in diverse melons showed SCAPB05 primers obtained a single 1,046 bp linked to Creb-2 in resistant cultivars Sanuki-shirouri and Kohimeuri. However, SCOPE14 failed to detect Creb-2 in diverse melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to CMV-B2 by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. Key words: Cucumis melo L., Creb-2, RAPD, SCAR. HAYATI J Biosci [Internet]. 1 [cited 2024May3];16(4):142. Available from: https://journal.ipb.ac.id/index.php/hayati/article/view/%20%20%20%3Cp%20class%3D%22MsoNormal%22%20style%3D%22text-align%3Ajustify%3Btext-indent%3A14.15pt%3B%22%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3ETwo%20random%20amplified%20polymorphic%20DNA%20%28RAPD%29%20markers%20linked%20to%20CMV-B2%20resistance%20gene%20%28%3Cem%3ECreb-2%3C%2Fem%3E%29%20in%20melon%20cultivar%20Yamatouri%20were%20cloned%20and%20sequenced%20to%20design%20sequence%20characterized%20amplified%20region%20%28SCAR%29%20markers%20for%20detection%20of%20CMV-B2%20resistance%20gene%20%28%3Cem%3ECreb-2%3C%2Fem%3E%29%20in%20melon.%20SCOPE14%20derived%20from%20OPE-14%20yielded%20a%20single%20DNA%20band%20at%20541%20bp%2C%20while%20SCAPB05%20derived%20from%20APB-05%2C%20yielded%20a%20single%20DNA%20band%20at%201%2C046%20bp%2C%20respectively.%20Segregation%20of%20SCOPE14%20and%20SCAPB05%20markers%20in%20bulk%20of%20F%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A5pt%3B%22%3E2%3C%2Fspan%3E%3C%2Fstrong%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3E%3Cspan%3E%C2%A0%3C%2Fspan%3Eplants%20demonstrated%20that%20they%20were%20co-segregated%20with%20RAPD%20markers%20from%20which%20the%20SCAR%20markers%20were%20originated.%20Furthermore%2C%20results%20of%20SCAR%20analysis%20in%20diverse%20melons%20showed%20SCAPB05%20primers%20obtained%20a%20single%201%2C046%20bp%20linked%20to%20%3Cem%3ECreb-2%3C%2Fem%3E%20in%20resistant%20cultivars%20Sanuki-shirouri%20and%20Kohimeuri.%20However%2C%20SCOPE14%20failed%20to%20detect%20%3Cem%3ECreb-2%3C%2Fem%3E%20in%20diverse%20melons.%20Results%20of%20this%20study%20revealed%20that%20SCAR%20analysis%20not%20only%20confirmed%20melons%20that%20had%20been%20clearly%20scored%20for%20resistance%20to%20CMV-B2%20by%20RAPD%20markers%2C%20but%20also%20clarified%20the%20ambiguous%20resistance%20results%20obtained%20by%20the%20RAPD%20markers.%3C%2Fspan%3E%3C%2Fstrong%3E%3C%2Fp%3E%20%3Cp%20class%3D%22MsoBodyText2%22%20style%3D%22line-height%3A10.8pt%3B%22%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3E%C2%A0%3C%2Fspan%3E%3C%2Fstrong%3E%3C%2Fp%3E%20%3Cp%20class%3D%22MsoBodyText2%22%20style%3D%22margin-left%3A58.1pt%3Btext-indent%3A-58.1pt%3Bline-height%3A10.8pt%3B%22%3E%3Cstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3E%3Cspan%3E%C2%A0%C2%A0%C2%A0%C2%A0%C2%A0%20%3C%2Fspan%3E%3C%2Fspan%3E%3C%2Fstrong%3E%3Cspan%20style%3D%22font-size%3A9pt%3B%22%3EKey%20words%3A%20%3Cem%3ECucumis%20melo%3C%2Fem%3E%20L.%2C%20%3Cem%3ECreb-2%3C%2Fem%3E%2C%20RAPD%2C%20SCAR%3Cstrong%3E%3C%2Fstrong%3E%3C%2Fspan%3E%3C%2Fp%3E