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SULANDARIS.; ZEINM. S. A. Visually Identifying the Sex of a Bird Can Be Difficult. It Cannot Be Done in Half the world’s Species When They Are Adults, and Virtually None Can Be Sexed As Chicks. Despite This, the Sex of a Bird Is Vital for Captive Breeding. An Increased Number of Birds Are Being Sexed Using DNA Amplification Techniques. In This Approach, the CHD-W and CHD-Z Are Distinguished by the Amplification of an Intron Present in Both Genes. PCR Products on the Gel Electrophoresis Vary in Size Revealing One Band in Males at the CHD-Z, and Two Bands in Females Corresponding to Both the CHD-W and CHD-Z. Two Independent Sets of Primer (P8/P2 and 2550F/2718R) Were Used to Amplify the CHD Gene Region from Both the Z and W Chromosome. One Hundred and Ten (110) Birds Were Sexed Using First Pair of Primers: (P8/P2). Sexing Results Indicated That 81.8% Were Successfully Determined, 12.7% Failed to Be Amplified and 5.5% were Not Perfectly Determined Because the PCR Products Showed Thick Band. The Thick Band Caused Misidentified Female to Male Birds. An Alternative Primer (2550F/2718R) Was Applied to Solve the Problem. Two Hundreds and Twenty-Nine Birds Were Sexed and the Results Showed 100% Successfully Determined. From This Study, It Is Suggested to Use a Pair of 2550F and 2718R Primers for Distinguishing a Male from a Female bird. . HAYATI J Biosci 1, 19, 183.