TY - JOUR AU - Al-Mughni, Eka Wasi’ AU - Daryono, Budi Setiadi PY - 2020/04/01 Y2 - 2024/03/28 TI - Molecular Characterization and Phylogenetic Relationships of Melon (Cucumis melo L. Meloni Cultivar) Based on Expression of Andromonoecious Gene JF - HAYATI Journal of Biosciences JA - HAYATI J Biosci VL - 27 IS - 2 SE - Short Communication DO - 10.4308/hjb.27.2.174 UR - https://journal.ipb.ac.id/index.php/hayati/article/view/31501 SP - 174 AB - Melon (Cucumis melo L.) is one of horticultural commodities with good economic prospect for melon farmers because of several advantages. 'Meloni' is result of crossing between ‘Sun Lady-3’ and ‘PI 371795’ by Genetics and Breeding Laboratory, Faculty of Biology, UGM. The 'Meloni' plant produces females flowers that expressed by CmACS-7 gene and it can be expected that melon plants 'Meloni' included in the primitive cultivars, while 'Meloni' is a cultivation cultivar. The aim of this research is to study the relation of 'Meloni' kinship to primitive and modern cultivars. Characterization of the CmACS-7 gene is begun by a total isolation process of RNA from a sample of melon plant flowers and detection using a specific primer. The cDNA bands obtained are sequenced and then phylogenetic analysis is performed based on CmACS-7 gene expression using MEGA 7.0. Result of this study show that the presence of the CmACS-7 gene in female and hermaphrodite flowers of ‘Meloni’, ‘PI 371795’, ‘Sun Lady-3’, and ‘Hikapel’. Phylogenetic tree analyze show that ‘Meloni’ from female plant, ‘PI 371795’ and ‘Sun Lady-3’ have closely relation, while 'Hikapel' separated from other cultivars. ER -